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Description
Human IL-7 OneStep ELISA KitProduct Specification Antigen IL 7 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 3. 7% Inter assay: 6. 0% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 653 pg ml Range 1. 56 pg mL 100 pg mL Recovery Cell culture supernatant: 115%
Product Specification
| Antigen | IL-7 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.7%； Inter-assay: 6.0% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.653 pg/ml |
| Range | 1.56 pg/mL – 100 pg/mL |
| Recovery | Cell culture supernatant: 115% Serum: 98% Plasma: 114% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide. Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Like other common gamma-chain cytokines like IL-2 and IL-15, IL-7 and its receptor, IL-7R, have been used in a variety of immunotherapy applications, often in fluid tumors and in some instances of solid tumor models.
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