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Description
Human SMN2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Survival of Motor Neuron 2, Centromeric (SMN2). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Survival of Motor Neuron 2, Centromeric (SMN2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Survival Of Motor Neuron 2, Centromeric | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Survival motor neuron 2, also known as SMN2, is encoded by the SMN2 gene. The SMN2 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repeated elements, making it prone to rearrangements and deletions. The repetitive nature and complexity of the sequence also make it difficult to determine the organization of this genomic region. The telomeric (SMN1) and centromeric (SMN2) copies of the gene are nearly identical and encode the same protein. The key sequence difference between the two genes is a single nucleotide in exon 7, which is thought to function as an exonic splicing enhancer. Nucleotide substitutions in SMN2 result in approximately 80-90% of its transcripts being truncated, unstable, and nonfunctional (Δ7SMN), while only 10-20% of transcripts are full-length (fl-SMN). While mutations in the telomeric copy are associated with spinal muscular atrophy, mutations in the centromeric copy of this gene do not cause disease. This gene may be a modifier of diseases caused by mutations in the telomeric copy. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Great Cleaning Power and Super Convenient
Color: White, Size: D20Plus, Color: White, Size: D20Plus
The DREAME D20 Plus Robot Vacuum and Mop works really well in my apartment. The suction is very strong and easily picks up dust and pet hair from both carpets and hard floors. I also like the self-emptying feature with the large dust bag, which saves me a lot of time. The mapping is accurate, and the battery lasts long enough to clean the whole place. Overall, it’s a great robot vacuum for the price.
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Reviewed in the United States on March 12, 2026
★★★★★ 5
I am very satisfied with the overall experience!
Color: Black, Size: D20Plus, Color: Black, Size: D20Plus
The suction power is really strong. Daily dust, hair and debris can be easily cleaned up, and the ground is obviously much cleaner. Although there are no pets at home, it is enough for daily cleaning.
What I like most is its self-dust collection function. The 5L large dust bag has a large capacity and can be used for a long time without dumping garbage. It is really worry-free. It is very friendly to people who do not want to take care of the machine frequently.
The drawing is very fast and accurate, and the cleaning route is orderly and will not collide randomly. The APP is easy to operate, and the appointment and remote control are very convenient.
Generally speaking, it is very cost-effective, time-saving and effort-saving, and worth buying.
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Reviewed in the United States on March 30, 2026
★★★★★ 5
Good Robot Vacuum
Color: Black, Size: D20Plus, Color: Black, Size: D20Plus
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Incredible robot.
Size: 11S MAX, Size: 11S MAX
I have had this vacuum for nearly a year now, running it on an automatic schedule every single day, and I am so grateful for this product and how it has improved my quality of life.
This vacuum and its automatic schedule has meant that I rarely see any dust or dog hair on my floor and I no longer have to take the time to vacuum. The amount of dust this machine collects is unbelievable- the photo I included of the yield is from HALF of a cycle.
Flooring/obstacles
I use my eufy on hard floor and short carpet and it works wonderfully. It does snag on high pile rugs so I try to put my bathroom mat up when I remember.
Eufy does get stuck on things so it took some time to determine what picking up I need to do before it’s “shift.” This has not been a nuisance, it’s more of a good motivation to do a quick cleanup.
Durability
Also, long hair is a challenge and I do have to cut it out of the brush head about once a week. I also recently replaced the brush head which was a great reset. The side spin brush also broke down over a few months and replacing that REALLY improved the power of the machine. I’ll be replacing it as soon as I notice breakage now.
Noise
I think it has gotten a bit louder over the year I have owned it. I used to be able to sleep through the noise, now it’s a bit too loud for me especially as it gently bangs against the walls (doesn’t scuff though).
Overall, this is a gift and I will never not have one for the rest of my life
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Reviewed in the United States on May 19, 2026
★★★★★ 5
BEST ROBOVAC EVER!
Size: 11S MAX
This is BEST Robovac you'll ever own, IMHO.
I purchased the first robovac made by a different company when these little things were a completely new concept. This is BEST Robovac you'll ever own, IMHO. I purchased the first robovac made by a different company when these little things were a completely new concept. It was such a surprise as to how good it was, and how it made a difference in time spent housekeeping every day. I've been a fan ever since (20+ yrs) and have tried many different brands and models. The Eufy is the best! And I have purchased 3 of this model.
Depending upon your budget and specific needs, there are things I've learned, and I'm going to break those points down for your consideration. Possibly they may apply to your situation. These points are some requirements that make all the difference to me and my lifestyle. ~~>
#1. Two auxiliary brushes.: This is the most important thing for me and is a no brainer, IMHO. The two brushes, one on each side of the front edge (approx 11 and 2 o'clock) sweep the debris to the underneath center of the unit where it is sucked up. Because there are two, they sweep a larger area, and because the vac covers the same areas more than once, you get better cleaning results. This is very important in kitchens and high traffic areas. Also corners are swept from both sides.
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#4. Rechargeable on its own! Need I say more? It goes back to the recharging station to recharge itself when it needs more juice. YAY! We live on three floors, and this is a huge plus for us.
#5. The Customer service with this company is amazing! If you have any issues, regardless of how small, they will get back to you, and immediately help. They respond, and do not merely send you to a FAQ link.
#6. It is gentle and does not smack into your furniture, scuffing it.
#7. The more you use it, the cleaner your house will be. Regular dusting is done on a daily basis so there is less and less dirt and dust to deal with. It is amazing the difference! Weekend freedom!
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My Eufys are my workhorses and I recommend this little gem of a vac if you are considering it. IMHO It will be a good investment. It has been for me, and my family (including cats and dogs).
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Reviewed in the United States on March 13, 2025