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Description
Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. 2. Use of secondary antibody: This kit is matched with a universal secondary antibody for pika and rabbits, which is not suitable for mouse tissues. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50030) for mouse tissue samples. 3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:abs994) |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165729501.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.0 ★★★★★
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Product Reviews
★★★★★ 5
Great value to protect your ipad.
Color: Black, Size: Case for iPad (A16) 11th/10th Generation 11/10.9''
Nicely made and thought out product. Fits perfectly and comes with everything you need. Easy to install, looks very sturdy. The shoulder strap it comes with to carry it around makes it even more versatile.
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Reviewed in the United States on April 22, 2026
★★★★★ 5
It works 100% as intended plus benefit of stand that changes with ease position of screen
Color: Black+Red, Size: Case for iPad (A16) 11th/10th Generation 11/10.9''
My tendency to knock over anything at least two inches high is a remarkable skill I’d rather not have but as long as I can claim said skill, I’m eternally grateful for this case for my iPad. Not only is it the only protective device that’s accomplished its intended use, it features a stand that is adjustable with ease to view your screen from any direction you prefer and can still access volume and off/on button. The leather strap to handle it with one hand also is a perfect solution to a typical problem. I’m accustomed to a cracked screen on iPhones and tablets or iPads but this iPad is the first I’ve ever had to go over six months and survive.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Cool Texture, Great Product, Notable Cons, Still Recommend
Model: Tab S11/S10 Lite/S10 FE/S9/S9 FE/S8/S7
AMAZING PRODUCT! I've never used a "paper-like" screen protector, but this one applied perfectly and was easy to use! (just follow the instructions!) no bubbles, just one single speck of dust (my own fault - sigh). It creates a very strong "matte" effect and feels cool to run your hand over (very paper-y)
CONS:
-The fit was SLIGHTLY big for my new S11 tablet, (each corner went RIGHT to the very corner of the tablet ALMOST going over the lip-this was just the corners of the protector and not the edges, which I found weird. IMO, its too close for my own comfort, but it still works none the less, and I've managed to use a case with it too with only minor corner lift).
-The texture of the screen protector definitely distorts the pixels slightly, its just something to get used to, and probably something you'd have to see yourself, for if it would bug you or not.
-It collects fingerprints like craaazy but they also wipe off craaazy easy too....sooo not fully an issue? just surprised me.
Overall, for the money, I still recommend getting this protector if you're at all interested in a "paper-like" finish!
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Great quality and easy to apply
Model: iPad A16 11th/10th/Air 11
I love these screen protectors. They feel amazing and they sent an installation kit making the applying process so easy. There was also a squeegee included to make sure air bubbles wouldn’t
Be a problem. I’ve had zero problems with the protector reducing the touch sensitivity of the screen, the protectors fit well, seem durable, and are great quality. The price was also reasonable. I’ve never owned other paper-feel screen protectors so I’m not able to give a comparison, but for these being my first ones I am very pleased.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Get this if you draw, write, play touch games
Model: iPad Pro 11,iPad Air 5th/4th
The quality of the screen protector is great, easy to put on without the video. i love how it really feels like writing on paper with my pen especially drawing. I will definitely be buying more for myself,mother and siblings. It’s a good fit for my iPad Air 4th gen and a bang for the buck
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Reviewed in the United States on May 25, 2026