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Description
MTT Cell Proliferation and Cytotoxicity Assay KitProduct Specification Usage 1. Place the cell plate in the sterile operating table. 2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg ml MTT solution. It can be used after preparation, or stored directly at 20 in the dark from light, or stored at 20 in the dark from light after appropriately packaging as needed.] 3. Put the cell plate back into
Product Specification
| Usage | 1. Place the cell plate in the sterile operating table. 2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg/ml MTT solution. It can be used after preparation, or stored directly at-20 ℃ in the dark from light, or stored at-20 ℃ in the dark from light after appropriately packaging as needed.] 3. Put the cell plate back into the incubator and continue to incubate for 3-4 hours. When doing comparative experiments, the incubation time should be consistent. 4. After incubation, take out the cell plate from the incubator and dissolve it according to the following method to produce MTT formazan crystals. (1) If it is an adherent cell, discard the culture medium. Add the same amount of Formazan Solvent as the initial volume. The volume of dissolved fluid may vary per well, but the total volume must be consistent for comparison. (2) If the removal of non-adherent cells or culture medium will cause the loss of MTT formazan, add the same amount of lysate as the initial volume directly to the culture medium. (3) Readings must be completed within 1 hour after addition of Formazan Solvent. 5. Gentle shaking in a shaker can enhance dissolution. In some cases, particularly when the cell density is high, repeated pipetting is required to completely dissolve the MTT formazan crystals. 6. Spectrophotometer to measure OD570 and reference wavelength OD690. 7. The 96-well plate can be detected on a microplate reader equipped with a suitable filter. 8. Other types of multi-well plates need to be spectrophotometrically detected with a cuvette or plate reader of appropriate size. |
| Beilstein | 0 |
| Synonym | MTT Cell Proliferation and Cytotoxicity Assay Kit,MTT kit |
| Description |
The MTT assay, also known as MTT colorimetry, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline Formazan and deposit it in cells, while dead cells do not have this function. Within a certain range of cell numbers, the amount of MTT crystals formed is directly proportional to the number of cells. The greater the absorbance, the stronger the cell activity, indicating that the drug toxicity is less. This method has been widely used in the activity detection of some bioactive factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination. It is characterized by high sensitivity and economy. Components: MTT (25mg), MTT solvent (5ml), Formazan solution (55ml)
|
| PubChem CID | 0 |
| General Notes | 1. Since a 96-well plate is used for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since the circle around the 96-well plate is the easiest to evaporate, you can discard the circle around the plate and add PBS, water or culture medium instead; On the other hand, a 96-well plate can be placed close to a water source in the incubator to alleviate evaporation. 2. If the test compound used can react with MTT, centrifuge first and then discard the culture medium. Carefully flush it with PBS for 2 to 3 times, and then add the culture medium containing MTT. 3. The production of Formazan is not only proportional to the number of living cells, but also affected by the action time. Therefore, when there are many samples, the measurement results may change with time. 4. This product contains carcinogens, avoid contact with skin and eyes, please operate carefully. For your safety and health, please operate in a lab coat and disposable gloves. 5. This product is only used for scientific research and cannot be used clinically or diagnostically. |
| Storage Temp. | Stored at 2 ~ 8 ℃, protected from light, valid for 12 months. |
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4.8 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 4
Nice fabric
Nice fabric, bad fit! Tank rides up
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 9, 2025
★★★★★ 3
Comparing BAMBOO COOL ribbed tank vs spaghetti strap cami
Color: 3-pack（blue,light Red,nude）, Size: X-Large-XX-Large, Color: 3-pack（blue,light Red,nude）, Size: X-Large-XX-Large
I really wanted to love these so I bought two styles to compare. The difference between the two is huge and I am not keeping either one.
The ribbed tank is nice and thick, which is nice. But it is too small and too short. The xxl is 14" across at the bottom. Compare that to a tank you like before buying. It is also too short and rolls up. It is 17" long, the description says it's 21".
The spaghetti strap cami is tissue thin. Like really, really, really thin fabric..... It is soft - but about 2 sizes too large. It is 22" across. I do like the length though. Far too thin to wear in lieu of a bra which was what I wanted them for.
Both styles are going back. One is too big, the other too small. One is too thin, the other too short.
Ordered xxl in both sizes.
The ribbed tank is 14" across at the waist.
The Spaghetti strap cami is 22" across at the waist and about 5 inches longer.
See photo.
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Reviewed in the United States on March 5, 2026
★★★★★ 5
Wardrobe staple!
These are PERFECT for summer but also will be great for layering in cooler weather. I bought 2 different packages so I have a plethora of colors to choose from. The fabric is extremely comfortable and great quality. I went with med/large because I'm a 36DD, 145 lbs and 5'6". I could've gone for the next size up, they're snug but not at all tight after wearing for a bit... and I can skip the bra! There's no side boob as they go quite high in the pit area. They're thick, not see through and fine ribbed so no visible nips. They rest right at my belly button and I'm pretty long waisted. I haven't washed them yet but not too worried because I don't dry most of my clothes anyway.
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Reviewed in the United States on July 23, 2025
★★★★★ 5
They work
Love them!!! I stay so hot and these really wick the moisture away!
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Reviewed in the United States on February 26, 2026
★★★★★ 2
Not for me
Returned. Too flimsy, did not like the strups shape, was not flattering or supportive in any way, white one is completely see through
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Reviewed in the United States on March 17, 2026
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