SKU: 77625649890

Human IL-10 High Sensitivity ELISA Kit

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Description

Human IL-10 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 10 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 10 in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.05pg/ml

Detection Range: 0.39-25 pg/ml

Recovery Range: 88-100%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL10 gene located on chromosome 1 and consists of five exons. IL-10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

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SKU: 77625649890

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44Magpie
Houston, US
★★★★★ 5
Great World Time Watch!
Color: Stainless/ Black Dial
The usual quality Citizen construction, Eco-Drive, and a very handy world time bezel. FIVE STARS!
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Reviewed in the United States on February 8, 2026
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DS
Los Angeles, US
★★★★★ 4
Great watch for the money
Color: Stainless Mesh/ Blue Dial
Looks great. I swapped out the band for a leather strap. Nice watch, well designed. My other eco drive has been going strong for 15 years or so without a hitch. So I have high expectations for this one.
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Reviewed in the United States on January 9, 2024
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Gael Rivera
Whiting, US
★★★★★ 5
Great Gift
Color: Stainless Mesh/ Blue Dial
It was a gift for my Dad but he loved it! Very worth it
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Reviewed in the United States on April 23, 2026
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Anthony
Fort Morgan, US
★★★★★ 5
Gotta have a citizen watch
Color: Stainless/ Black Dial
Fell in love with this watch..
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Reviewed in the United States on February 22, 2026
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Vincent Petralia
Charlottesville, US
★★★★★ 1
Flimsy, small face
Color: Stainless Mesh/ Blue Dial
This watch doesn’t feel like the typical eco drive. The face is small, it’s very light and the mesh band was a pain to clasp. I returned it the day I got it.
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Reviewed in the United States on January 5, 2024

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