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Description
Aurora-B Recombinant Rabbit mAb (SDT-079-32)Product Specification Host Rabbit Antigen Aurora B Synonyms Aurora 1, AIM 1, ARK 2, STK 1, Aurora related kinase 2 Immunogen Synthetic Peptide Accession Q96GD4 Clone Number SDT 079 32 Antibody Type Rabbit mAb Application WB, IHC P, ICC, ICFCM, IP Reactivity Hu Purification Protein A Concentration 0. 5mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability & Storage 12 months
Product Specification
| Host | Rabbit |
| Antigen | Aurora-B |
| Synonyms | Aurora 1, AIM-1, ARK-2, STK-1, Aurora-related kinase 2 |
| Immunogen | Synthetic Peptide |
| Accession | Q96GD4 |
| Clone Number | SDT-079-32 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IP | 1:25 | |
| IHC-P | 1:500-2000 | |
| WB | 1:1000 | |
| ICC | 1:500 | |
| ICFCM | 1:5000 |
Background
Aurora kinase B is a protein that functions in the attachment of the mitotic spindle to the centromere. The expression and activity of Aurora B are regulated according to the cell cycle. Expression of Aurora B reaches a maximum at the G2-M transition, whereas Aurora B protein is most active during mitosis. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes. In cancerous cells, over-expression of these enzymes causes unequal distribution of genetic information, creating aneuploid cells, a hallmark of cancer.
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