Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Sep 4 - Sep 9
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TIEG1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against TGF Beta Inducible Early Response Gene 1 (TIEG1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of TGF Beta Inducible Early Response Gene 1 (TIEG1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TGF Beta Inducible Early Response Gene 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | TIEG1/KLF10 (TGFβ-induced early response gene 1, Kruppel-like factor 10) is a transcriptional regulator involved in controlling cell growth. KLF10 binds to Sp1/GC-rich DNA sequences and can activate or repress the transcription of several genes. It is particularly important for osteoblast function, including bone mineralization and supporting osteoclast differentiation. KLF10 is rapidly induced by TGFβ, bone morphogenetic protein, estrogen, and epidermal growth factor. It plays a major role in TGFβ-mediated inhibition of cell proliferation and inflammation, as well as induction of apoptosis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 13 reviews
Sort
Product Reviews
★★★★★ 5
Wheels role, comfortable, easy to assemble and s
Color: Black, Size: 1 Pack
This has rolling wheels and if I could put it together Anyone can. It fits me fine (250 pounds) the seat is comfortable. They gave me all of the tools (1 tool) needed. Directions were pictures and words that are large enough for me to read. I liked the lower cost and the shipping packaging was great. I’m too big to even try the recliner. I’m sure it would do it but I have had some bad experiences in the past. Don’t be fooled by reviews that state the wheels don’t move. They do. I would buy this chair again
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 4
Office Chair
Color: Grey, Size: 1
This chair is the perfect size for a small space however it was a nightmare to assemble.! The screws did not align well and some of them I couldn't tighten at all. Overall I am very pleased with the purchase it's very comfortable and sturdy for the price. I just wish the arms were a little bit higher though...
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026
★★★★★ 5
Nice
Color: Black, Size: 1 Pack
Nice chair. Easy to assemble. Comfortable enough. Arm rests are nice. Would buy again
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
★★★★★ 1
total complete junk.
Color: Black, Size: 1 Pack
STOP!!! Do NOT click on Buy. This is simply the biggest piece of **** you've ever seen. Impossible to put together because all the threaded inserts are misaligned. PLUS, it came busted. One of the arm rests was cracked all the way through. And a piss poor design, too. The back attaches to the armrests which in turn connect to the base. Without three or four people, it's simply not possible to put this worthless excuse for a chair together. And don't even THINK about using it WITH the back but WITHOUT the armrests!! Can't do it. Not possible. Terrible design. Seriously, this is right at the top of the list of useless junk I've gotten from Amazon. Not Amazon's fault exactly, they just sell the crap and at least they make it super easy to return this idiotic junk. OK, maybe it IS their fault. Shipped and sold by Amazon so there's that.... Oh, it took over a week to get here, too so there's THAT as well.
Yup, looks like a great deal at first. But....after spending half your life trying to assemble it.... Nope. Not worth one damn cent. Utter absolute junk. Last warning: DO NOT BUY THIS RUBBISH!!! Don't you do it!! Clearly I loved this purchase.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 12, 2026
★★★★★ 5
extremely comfy
I’m in love with this chair. It’s extremely comfy and I can sit for long periods of time without any back pain. It’s easy to move around despite having no wheels. I have a plastic chair mat under my desk and it slides across it easily when I need to move it out of the way. I love the height of this chair as well. I am 4’11 and most chairs even at their lowest setting my feet don’t fully reach the floor comfortably. Usually my legs are leaned forward or on my toes a bit. However this chair at its lowest setting I can perfectly reach the floor and knees are at the comfiest 90 degree angle.
SETUP: The chair was fairly easy to put together. Thankfully I read the reviews or I would’ve been extremely frustrated. The instructions for this are literally 4 steps that make it seem super easy. It is but not because of the instructions. Another reviewer left a video review and said it’s easier to put together if you swap steps 4 and 3 so you can line up the holes on the back with the bottom easier. This is 100% true so thanks for that reviewer!!! I’ll add on - you have to put the back and bottom together at kind of a weird angle to get the holes to line up and start with the back holes first! I can’t even really describe the angle but it feels really wrong. Almost like a wider angle than you think it should ever go. The back of the chair isn’t a 90 degree angle it tilts back a bit. If you can find the angle you’ll get the screws in with very little issue. I put this together by myself in about 5 minutes and 4 of those minutes were figuring out the angle for the back of the chair.
CONS: I only have one con really. It’s the angle of the back of the chair. It’s tilted back just a tad too much for me. I like to be able to sit back in my chair and lean into the back but with this one if I do that I can’t type very well. I think if I add a lumbar support pillow I can lean back comfortably. To me, it’s not a huge deal because of the overall comfort and price of this chair. But something worth mentioning.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 8, 2024