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Description
Alexa Fluor® 488 Mouse Anti-Human Perforin Antibody (S-3064)Product Specification Host Mouse Antigen Perforin Synonyms Perforin 1; P1; Cytolysin; Lymphocyte pore forming protein (PFP); PFP; PRF1 Location Cell membrane Accession P14222 Clone Number S 3064 Antibody Type Mouse mAb Isotype IgG2b,k Application ICFCM Reactivity Hu Positive Sample Human PBMC Purification Protein A Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability &
Product Specification
| Host | Mouse |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein (PFP); PFP; PRF1 |
| Location | Cell membrane |
| Accession | P14222 |
| Clone Number | S-3064 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions as a pore-forming protein, inserting itself into the membranes of target cells, such as virus-infected or cancerous cells. Once integrated, perforin creates pores that disrupt the cell membrane's integrity, leading to osmotic lysis and ultimately killing the target cell. This mechanism is essential for the immune system's ability to eliminate harmful cells and maintain homeostasis.
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