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Description
UniOne® TR-FRET Human IL6/IL6R Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit employs Homogeneous Time Resolved Fluorescence (TR FRET) technology to measure the interaction between IL 6 and IL 6R. This method enables simple, rapid, and high throughput identification of inhibitors and antibody based blockers. As illustrated, the interaction between IL 6 and IL 6R is detected using a Eu labeled anti Tag1 antibody (TR FRET donor) and an Acceptor
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
The assay kit employs Homogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interaction between IL-6 and IL-6R. This method enables simple, rapid, and high-throughput identification of inhibitors and antibody-based blockers. As illustrated, the interaction between IL-6 and IL-6R is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Upon binding of IL-6 to IL-6R, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptor antibody, resulting in specific emission of a 665 nm wavelength signal from the acceptor. The intensity of this specific signal is proportional to the extent of IL-6/IL-6R interaction. This homogeneous assay features a straightforward workflow with no washing steps required.
Components
Table 1. Reagents and Storage Conditions
Component |
Concentration |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|
Tag1-IL6 protein |
100× |
20μL |
100μL |
400μL |
-80°C |
Tag2-IL-6R protein |
100× |
20μL |
100μL |
400μL |
-80°C |
Tocilizumab |
10 μM |
10 μL |
50 μL |
200 μL |
-80°C |
Anti-Tag1 Eu cryptate antibody |
50× |
50μL |
250μL |
1000μL |
-80°C |
Anti-Tag2 Acceptor antibody |
12.5× |
200μL |
1000μL |
4000μL |
-80°C |
Detection buffer |
10× |
2mL |
10mL |
40mL |
-80°C |
* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first th
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