SKU: 86620593282

UniOne® TR-FRET Human BRD4/CRBN PROTAC Binding Kit

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Description

UniOne® TR-FRET Human BRD4/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the Molecular Glue BET Degrader 1 and testcompound mediated interaction between the DDB1 CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh throughput format for detecting small molecules capable of mediating theinteraction between the DDB1 CRBN

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the Molecular Glue BET Degrader-1 and testcompound-mediated interaction between the DDB1/CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh-throughput format for detecting small molecules capable of mediating theinteraction between the DDB1/CRBN complex and BRD4.

As shown, the interaction between DDB1/CRBN and BRD4 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the Molecular GlueBET Degrader-1 mediates the interaction between DDB1/CRBN and BRD4, the donorand acceptor antibodies are brought into proximity. Excitation of the donor antibodytriggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665nm. This specificsignal is proportional to the degree of interaction mediated by BET Degrader-1between DDB1/CRBN and BRD4. This homogeneous assay is simple to perform andrequires no washing steps.



Components

Component

Concentration

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

Tag1-DDB1/CRBN protein

25×

80μL

400μL

1600μL

-80°C

Tag2-BRD4 protein

100×

20μL

100μL

400μL

-80°C

BET Degrader-1 (100μM)

10μM

10μL

50μL

200μL

-80°C

Anti-Tag1 Eu dye antibody

50×

50μL

250μL

1000μL

-80°C

Anti-Tag2 Ac antibody

12.5×

200μL

1000μL

4000μL

-80°C

Detection buffer

10×

2mL

10mL

40mL

-80°C

*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.

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SKU: 86620593282

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4.2 ★★★★★
Based on 23 reviews
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Product Reviews
I
Verified Purchase
I read too much
Cuba, US
★★★★★ 5
Raw and Honest
Format: Hardcover
I admit I purchased this book because of all the hype, but also because I have been, since childhood, intrigued by her various family legacies (Burden, Vanderbilt, Cushing, Fairbanks), and their alliances (Whitney, Astor, Roosevelt, etc). I was not sure what to expect, but I am grateful I read this book. Being a queer man I probably am not the core audience for this book, I can attest it is still a great read. Ms. Burden is amazingly candid and honest in a way I don't think most people in her situation would be. I can already tell that there will probably be countless reviews about how privileged her life was even during and after her divorce, and how unrelatable this book is for many people, but then you are missing something. If you want to judge Ms. Burden for things she had no control over then you should examine your own life. My hat off to the author for being so open about such private matters in her life, and also for doing a great job shielding her children in life and in the book. I hope it is true that there is a movie/series being made that will star Gwyneth Paltrow. I love Gwyneth, and if the author won't play herself I think Gwyneth is an amazing choice for her poise, acting ability, and class. Can't recommend this enough! OH, I am curious to know if anyone who read this book read Wendy Burden's memoir Dead End Gene Pool. I LOVE that book, but it is so very very different, and I am curious to know if Wendy and Amanda get along irl.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
R
Verified Purchase
Rains Family Farm
Los Angeles, US
★★★★★ 5
Daily devotional with ease.
Format: Imitation Leather
This is a great little devotional book for men. I bought two, one for me and one for my son. I keep mine at my desk at work and read it each morning and I enjoy the insightful quotes and verses that help me start my day. It truly only takes about a minute or two to read through everything and the verses are actually written out on the page with the reference. I know there are a lot of devotionals out there that you can select from, but I like this one because it’s small and compact and acutely directed towards men. The small size is perfect, the price is excellent, and the daily devotionals are spot on.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 3, 2026
M
Verified Purchase
Matthew
Port Orchard, US
★★★★★ 5
Easy and fun to read! Cute and small, easy to travel with
Format: Imitation Leather
I’m obsessed with this book. Each devotion really does only take a minute to read. It’s compact so I leave it in my truck but sometimes I’ll bring it in and keep it by my bed. There’s a book mark ribbon that keeps your place if you get off track of your days and you can’t remember where you left off. And I also like HOW the messages are explained. I grew up in church and I’m always like “hmm that makes sense now that it’s put that way!” lol
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 1, 2026
N
Verified Purchase
Niles M
Battle Creek, US
★★★★★ 5
The Answer
Format: Imitation Leather
I purchased this book of Devotions as I was looking for a guide that would be simple to follow and compact. It seemed like I always was making excuses for not being as diligent with my faith. It was easy to tell my self that I could not keep up with God or travel with a guide as it was too inconvenient or bulky. From a content and simplicity to stay focussed, this was the source I needed. From a convenience standpoint, it could not be any better. I had tried apps and digital versions previously and it just wasn't the same. Holding a book and leafing through the pages just feels so much better to me. A definite must for those of you looking for a impactful, yet small and convenient way to continue the journey.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
R
Verified Purchase
Rick
Belleville, US
★★★★★ 5
One minute with god
Format: Imitation Leather
Perfect way to start my morning. Spiritual and uplifting.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 2, 2026

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