SKU: 35878630826

UniOne® TR-FRET Human CD40/CD40L Binding Kit

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Description

UniOne® TR-FRET Human CD40/CD40L Binding KitProduct Specification Host Human Stability & Storage Store the kit at 80C or below. When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze thaw cycle; the labeled antibody solutions canundergo one additional freeze thaw cycle. To avoid repeated freeze thaw cycles,it is recommended to aliquot the remaining stock solutions into

Product Specification


Host Human
Stability & Storage

Store the kit at -80°C or below.When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze-thaw cycle; the labeled antibody solutions canundergo one additional freeze-thaw cycle. To avoid repeated freeze-thaw cycles,it is recommended to aliquot the remaining stock solutions into single-usecontainers for storage at -80°C. Thawed dilutions and Detection Buffer can bestored at 2-8°C.


Background

This kit utilizes HomogeneousTime-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology tomeasure the interaction between CD40 and CD40L. This method enables simple andrapid high-throughput screening of inhibitors and blocking antibodies.

As illustrated, the interactionbetween CD40 and CD40L is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRET acceptor).The binding of CD40 to CD40L brings the donor and acceptor antibodies intoproximity. Excitation of the donor antibody then triggers FRET to the acceptorantibody, resulting in a specific emission signal at 665 nm. The intensity ofthis specific signal is proportional to the degree of CD40/CD40L interaction.This homogeneous assay is simple to perform and requires no washing steps.



Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

Tag1-CD40 protein

100×

4μL

20μL

100μL

400μL

-80°C

Tag2-CD40L protein

100×

4μL

20μL

100μL

400μL

-80°C

Selicrelumab (12.5μM)

100×

3μL

10μL

50μL

200μL

-80°C

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1mL

-80°C

Anti-Tag2 Acceptor antibody

12.5×

40μL

200μL

1mL

4mL

-80°C

Detection buffer

10×

400μL

2mL

10mL

40mL

-80°C

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SKU: 35878630826

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4.5 ★★★★★
Based on 21 reviews
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E
Verified Purchase
Erik Okerholm
Alexandria, US
★★★★★ 5
Nice, sturdy, and supportive and easy to set up for my dual 27" monitors!
Size: Dual, Style: Free-Standing
Nice, sturdy, and supportive and easy to set up for my dual 27" monitors!
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Reviewed in the United States on May 18, 2026
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Verified Purchase
Needsmorenails
Phoenix, US
★★★★★ 5
Easily supports a pair of 26" displays
Size: Dual, Style: Free-Standing
I have no complaints with this stand. It is not as nice as the Inland brand sold at MicroCenter, but it does its job. It beats the price of the MicroCenter one, thats for sure. It comes with all the hardware you might need, even long screws and bushings if you have a display that needs them. Assembly was quick. It comes mostly assembled anyway. You have to put the bottom on the post then attach the assembled arms to the post. Setting up can be a challenge but with help, its easy. After mounting the bracket on the back of the displays, you just slide them into the receiver on the arms. Once mounted, adjusting the position is simple, but can take a while as there are several joints which can be positioned to angle the displays as you like. The cord clips do an OK job of cord management, but the clips are not as good as the Inland ones. They do the job, but not as durable. I broke the one that goes on the post, but I tried getting it to hold a wad of wire. My fault. I recommend this stand.
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Reviewed in the United States on December 3, 2025
J
Verified Purchase
Joshua Jordan
Draper, US
★★★★★ 5
It's simple, it works, & its price makes for a good deal
Size: Single, Style: Free-Standing
I needed a stand for a 27" VIZIO TV that had been wall-mounted for a few years, & (?) the original stand must've got the toss long ago... It's hard to say... I found this stand on Amazon priced right, plus it had a coupon attached to the listing. I scrolled down & I saw a "newer model" of the same product was available, so I was initially hesitant. But, looking at the product pictures, reading the description, & scanning through some of the reviews, then the same with the "newer model" — the only difference I could see was mostly aesthetic (it also appears to have a desk mount included, which wouldn't have worked for me regardless of its purpose—either for conversion to a desk stand-off mount, or to further secure the stand....?) Eventually, I just thought "....it's a stand with a VESA mount; how do you **** that up...?" And I really couldn't think of anything...even if the VESA bracket was milled not to spec (it is milled to spec), I could have still made it work....(?) So, I made the purchase, received it, & 9-10 screws later, the TV had a stand. & I'm guessing a much more versatile stand than the original. The bracket that attaches the stand to the VESA mount can lower or raise the TV quite a bit. Even in it's highest position, it's still retains its sturdiness & remains stable. The baseplate has a decent amount of weight, and its overall design is well balanced to favor stability (to a tolerance; e.g., an old CRT monitor just isn't going to work). The stand is literally 4 basic pieces—the VESA bracket, an articulating bracket, a metal tube, & a metal baseplate. If you have a standard phillips screwdriver, then the stand is easy to assemble. If you have any experience with TV mounts, or TV stands, then it assembles in less than 15 minutes. Given what you get for the price, the ease of assembly, the added versatility, if you need a stand for a screen within the manufacturer's specific tolerances, then I would absolutely recommend this stand.
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Reviewed in the United States on July 19, 2021
V
Verified Purchase
viplarry
Lexington, US
★★★★★ 5
Decent Monitor Stand with Some Assembly Required
Size: Single, Style: Free-Standing
This monitor stand works pretty well and is a good value for the price. It’s sturdy and adjustable, which makes it perfect for a variety of monitors, including my 32-inch curved Samsung. The assembly is straightforward if you have a Phillips screwdriver and something to tighten bolts, like a wrench. It’s solid once it’s put together and holds my monitor securely. The phone holder could use some improvement. It doesn't adjust to face me directly, which is a bit annoying. The stand itself is great for saving desk space and providing better ergonomics, but it can be a bit of a hassle to assemble if you’re not handy with tools. Overall, a good buy if you need a reliable monitor stand.
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Reviewed in the United States on October 25, 2024
S
Verified Purchase
sirav
Lowell, US
★★★★★ 3
Works, Good product, but wouldn't recommend for specifically 3 27inch screens.
Size: Triple, Style: Free-Standing
I purchased this stand to have 3 27inch 2560x1440 monitors, with the side ones angled towards me in a typical 3-monitor setup often seen being used by racing or aviation simulator guys. This stand's arms for the two side monitors are not long enough to have said monitors angled more than 5 to 8 degrees meaning they are forced to be completely linear which is incredibly awkward to try to use because you can barely see or use the screen space on the far edges. 𝐖𝐢𝐭𝐡 𝐭𝐡𝐞𝐬𝐞 𝐬𝐢𝐳𝐞 𝐬𝐜𝐫𝐞𝐞𝐧𝐬, 𝐭𝐡𝐞 𝐨𝐧𝐥𝐲 𝐰𝐚𝐲 𝐭𝐨 𝐡𝐚𝐯𝐞 𝐞𝐯𝐞𝐧 𝐨𝐧𝐞 𝐚𝐭 𝐚𝐧 𝐚𝐝𝐞𝐪𝐮𝐚𝐭𝐞 𝐚𝐧𝐠𝐥𝐞 𝐟𝐨𝐫𝐜𝐞𝐬 𝐭𝐡𝐞 𝐨𝐭𝐡𝐞𝐫 𝐦𝐨𝐧𝐢𝐭𝐨𝐫 𝐭𝐨 𝐛𝐞 𝐢𝐧 𝐩𝐨𝐫𝐭𝐫𝐚𝐢𝐭 𝐦𝐨𝐝𝐞, 𝐤𝐢𝐧𝐝 𝐨𝐟 𝐦𝐞𝐬𝐬𝐢𝐧𝐠 𝐮𝐩 𝐭𝐡𝐞 𝐰𝐡𝐨𝐥𝐞 𝐩𝐨𝐢𝐧𝐭 𝐨𝐟 𝐛𝐮𝐲𝐢𝐧𝐠 𝐭𝐡𝐢𝐬. The product description should not say it can hold 3 27 inch screens because the amount of struggling I had to do to get it to be acceptable was ridiculous. The stand is... not heavy enough to safely hold monitors of this size either, and additional weight was required ( 2 10lb dumbbells in my case ) configuring the height of your monitors with this stand can be dangerous as well as they could fall down the central post so you have to be extremely careful, but, it is pretty easy other than that. It is a great option if you don't have a desk with an edge for typical stands ( I don't. ) You can place the stand anywhere, and you don't have to drill through your desk if you really don't want to. The cable clips are a very nice included bonus, and they sent me a replacement without any hassle when I totally effed up my first attempt at setup. But, I would have shopped harder and got something else if I knew all this prior all the same. Maybe even bought a gigantic curved screen instead.
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Reviewed in the United States on January 8, 2025

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