SKU: 25552881680

Rat IgA OneStep ELISA Kit

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Description

Rat IgA OneStep ELISA KitProduct Specification Antigen IgA Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Rt Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 7% Inter assay: 7. 1% Sample type Cell culture supernatant, Serum, Plasm Assay type Sandwich (quantitative) Sensitivity 0. 043 ng ml Range 1. 56 ng mL 100ng mL Recovery Cell culture supernatant: 99% Serum: 90% Plasm: 115% Assay time 60 minutes Species

Product Specification


Antigen IgA
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Rt
Purification Protein A
Stability & Storage 2 to 8 °C as supplied.

Kit


Precision Intra-assay: 5.7%;
Inter-assay: 7.1%
Sample type Cell culture supernatant, Serum, Plasm
Assay type Sandwich (quantitative)
Sensitivity 0.043 ng/ml
Range 1.56 ng/mL – 100ng/mL
Recovery Cell culture supernatant: 99%
Serum: 90%
Plasm: 115%
Assay time 60 minutes
Species reactivity Rat

Background

Immunoglobulin A (IgA) is a pivotal antibody isotype central to mucosal immunity and systemic immune regulation in rats. Structurally, rat IgA exists predominantly as a monomer in serum and as a dimer or polymer (secretory IgA, sIgA) at mucosal surfaces. Each IgA molecule comprises two α-heavy chains and two light chains (κ or λ), forming a flexible Y-shaped structure stabilized by disulfide bonds. The α-heavy chain contains three constant domains (Cα1–Cα3), with the Fc region (Cα2–Cα3) facilitating interactions with the Fcα receptor (FcαRI) and the polymeric immunoglobulin receptor (pIgR). Secretory IgA incorporates a J-chain and a secretory component, which enhance its stability in protease-rich mucosal environments. Extensive glycosylation of rat IgA further modulates its solubility, receptor binding, and resistance to enzymatic degradation. Rat IgA is the primary antibody safeguarding mucosal surfaces (e.g., respiratory, gastrointestinal tracts), where it neutralizes pathogens, blocks microbial adhesion, and maintains commensal microbiota balance. Secretory IgA mediates immune exclusion by trapping pathogens in mucus for mechanical clearance, while serum IgA contributes to systemic immune homeostasis by regulating inflammatory responses via FcαRI signaling. Additionally, rat IgA may engage in eosinophil-mediated antiparasitic defense and modulate allergic or autoimmune reactions through interactions with immune cells. Rat IgA shares conserved structural motifs with murine and human IgA, particularly in the α-chain constant domains. However, species-specific variations in glycosylation patterns, J-chain assembly, and secretory component binding necessitate species-specific assays for accurate detection. For example, rat IgA lacks the extended hinge region characteristic of human IgA1, resulting in distinct antigen accessibility and protease resistance. Clinical and Experimental Significance of IgA Levels: Elevated IgA: Associated with chronic mucosal infections (e.g., intestinal pathogens), autoimmune disorders (e.g., experimental colitis models), or inflammatory conditions. Reduced IgA: May indicate primary immunodeficiency (e.g., selective IgA deficiency in genetically modified models), immunosuppressive therapies, or gut dysbiosis. Applications of the Rat IgA ELISA Kit: This OneStep Rat IgA ELISA kit is designed for rapid and precise quantification of IgA in serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgA quantification in time-sensitive or resource-limited settings.

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SKU: 25552881680

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Dallas Johnson
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★★★★★ 5
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This volume delved way further than the last! The lore and context this gives to the whole universe is well worth it and gives lots to think about! Reveals how well planned and intricate this universe really is! Highest of recommends if you are into this franchise!
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Reviewed in the United States on July 1, 2025
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Sam
Draper, US
★★★★★ 2
Padding?
Format: Paperback
The book is a good quality, and the i like contents... besides the fact fully one third of the book is just the storyboard and script, with some sketches. Im used to covers and other supplemental materials taking up a few pages at the end, but that's ridiculous. Its either somewhat scummy padding or a complete mismatch in what i want and alot of other folks want i guess.
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Reviewed in the United States on April 11, 2026
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CB
Lake Worth, US
★★★★★ 5
got it
Format: Hardcover
got it when i was supposed to but can't review it anymore as it was a gift. it would be nice to just be able to star something & not HAVE TO write 20 words.
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Reviewed in the United States on May 30, 2013
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cormamin
Bozeman, US
★★★★★ 5
Great gift!
Format: Hardcover
Really nice quality, good illustrations. My boyfriend loved it.
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Reviewed in the United States on January 29, 2016
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Eric M Skoglund
Battle Creek, US
★★★★★ 5
Five Stars
Format: Hardcover
Great, got it autographed by hank Azaria, the voice of moe
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Reviewed in the United States on March 19, 2015

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