SKU: 3517616833

Human Serum Albumin Competitive OneStep ELISA Kit

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Description

Human Serum Albumin Competitive OneStep ELISA KitProduct Specification Antigen Serum Albumin Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 3. 7% Inter assay: 7. 4% Sample type Cell culture supernatant, Serum,Plasma Assay type Competitive (quantitative) Sensitivity 3. 56 ng ml Range 39. 06 ng mL 2500 ng mL Recovery Cell culture supernatant: 100% Serum: 95% Plasma: 96% Assay time 60

Product Specification


Antigen Serum Albumin
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage 2 to 8 °C as supplied.

Kit


Precision Intra-assay: 3.7%;
Inter-assay: 7.4%
Sample type Cell culture supernatant, Serum,Plasma
Assay type Competitive (quantitative)
Sensitivity 3.56 ng/ml
Range 39.06 ng/mL – 2500 ng/mL
Recovery Cell culture supernatant: 100%
Serum: 95%
Plasma: 96%
Assay time 60 minutes
Species reactivity Human

Background

Human Serum Albumin (HSA) is a single polypeptide chain composed of 585 amino acids, with a molecular weight of ~66.5 kDa. It is rich in cysteine residues (17 disulfide bonds) and lacks carbohydrates, making it highly soluble and stable. The protein folds into a heart-shaped tertiary structure with three homologous domains (I, II, and III), each divided into two subdomains (A and B). The structure is stabilized by disulfide bonds and hydrophobic interactions. HSA has multiple ligand-binding sites, notably for fatty acids, hormones, and drugs, located in hydrophobic pockets and flexible loops. HAS is a vital multifunctional plasma protein critical for homeostasis. It maintains ~80% of plasma colloid osmotic pressure to prevent vascular fluid leakage, transports hydrophobic molecules (e.g., fatty acids, hormones, drugs) and metal ions (Ca²⁺, Cu²⁺), and exerts antioxidant activity via its Cys-34 thiol group to neutralize free radicals and bind pro-oxidant metals. HSA also buffers blood pH using histidine residues and supports endothelial stability by regulating vascular permeability and nitric oxide bioavailability, thereby modulating blood flow. These roles collectively highlight HSA’s indispensable contribution to systemic health and disease regulation. HSA exhibits significant evolutionary conservation, sharing high sequence homology with serum albumin in other mammals. For instance, it shows ~76% sequence identity with Bovine Serum Albumin (BSA) and ~78% identity with Mouse Serum Albumin, highlighting its structural and functional preservation across species. This homology extends to its three-domain architecture (domains I, II, and III) and the characteristic disulfide bond patterns, both of which are remarkably conserved. low levels, caused by reduced synthesis (e.g., liver cirrhosis), excessive loss (e.g., nephrotic syndrome), malnutrition, or inflammation (e.g., sepsis), leads to edema, ascites, impaired drug efficacy, and poorer outcomes in critical illnesses. Conversely, elevated levels, typically due to dehydration (hemoconcentration) or rare genetic factors, is often transient but may signal chronic inflammation or metabolic disorders if persistently elevated. Applications of the Human Serum Albumin Competitive OneStep ELISA Kit: Human Serum Albumin Competitive OneStep ELISA kit enables rapid, accurate quantification of Serum Albumin in serum, plasma, or cell culture supernatants. Advantages of this Competitive OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Serum Albumin quantification in time-sensitive or resource-limited settings.

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SKU: 3517616833

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4.1 ★★★★★
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Verified Purchase
jeniene
Los Angeles, US
★★★★★ 4
Durable!
Color: 1 PC-Beef-Red
Alright, here's a product review for those Tough Dog Toys: Finally, a Toy That Survives My Monster! - Tough Dog Toys Review As the owner of a determined (and I mean determined) large breed chewer, finding toys that last longer than five minutes has been a constant and often frustrating quest. I've gone through countless plush toys ripped to shreds, rubber toys with chunks missing, and even some "indestructible" toys that met their demise far too quickly. That's why I was both hopeful and skeptical when I came across the Tough Dog Toys, designed specifically for aggressive chewers and large breeds. Let me tell you, these bone-shaped nylon toys are living up to their name. My power-chewing Labrador has been going at this thing for days now, and I'm genuinely impressed. Where other toys have succumbed to his relentless gnawing, the Tough Dog Toy has held its own. There are some minor teeth marks, as expected, but absolutely no significant damage, no pieces torn off, and no signs of imminent destruction. "Almost indestructible" might actually be an understatement! The design is simple but effective. The solid nylon construction feels incredibly durable, and the bone shape is easy for my dog to grip and maneuver. It's also a good size for a large breed – substantial enough that he can really get a good chew going without it disappearing in his mouth. What I appreciate most is the peace of mind this toy provides. I no longer have to constantly supervise playtime, fearing that he'll ingest pieces of a destroyed toy. This feels like a safe and long-lasting option for even the most enthusiastic chewers. Pros: * Truly durable – stands up to aggressive chewing from a large breed. * Solid nylon construction feels virtually indestructible. * Safe design with no small parts to break off. * Good size and shape for large dogs to grip and enjoy. * Provides long-lasting entertainment. Cons: * May show some teeth marks over time (though this hasn't compromised its integrity). * It's a fairly hard material, so dogs who prefer softer toys might not be as interested. Overall: If you're at your wit's end trying to find a toy that can withstand your aggressive chewing large breed dog, I highly recommend giving the Tough Dog Toys a try. This bone toy has proven to be the most durable option we've encountered, offering excellent value for money and, most importantly, a safe and engaging chewing experience for your furry friend. Five out of five paws!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
A
Verified Purchase
Amazon Customer
Fort Morgan, US
★★★★★ 5
Very well made chew bone!
Color: 1 PC-Beef-Red
Great durable, very well made chew bone! Our dog usually tears up all his toys within a few minutes or sometimes hours after he gets them. He’s had this bone for a few days and it’s still in one piece. Except for the rubber that’s in the middle, he chewed up in a couple of days. I would definitely buy more chew toys made by this company! Well worth the money!
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Reviewed in the United States on May 30, 2026
P
Verified Purchase
Pittieluv79
Pawtucket, US
★★★★★ 3
The rubber middle is not durable, it lasted less than 3 hours.
Color: 1 PC-Beef-Red
I bought this as I have bully/husky puppies (9 months old) and a pitt/doberman (9 years old). They are heavy chewers and destroy everything. I saw a video of this toy. The woman said that her dog chewed on it for a while (about 2 hours or so) she showed the ends visibly chewed, which I expect but it was holding up. The rubber middle seemed to hold up to. I received this yesterday. I had to take it away from them last night as they were chewing the rubber middle off. They had it maybe 3 hours. I didn't give it a lower star score as I will just cut the rubber off and give it back to them. So I was disappointed in the rubber middle not being more durable but the hard plastic it's made from is the strong material they need.
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Reviewed in the United States on May 16, 2026
B
Verified Purchase
BLACKHAWKS
Lake Worth, US
★★★★★ 5
Bones
Color: 1 PC-Beef-Red
Yes bone 🍖 is very strong and durable MIA, Rocky and Rambo love them ..
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Reviewed in the United States on June 9, 2026
L
Verified Purchase
Lexi
Waukegan, US
★★★★★ 4
Smaller than pictured but still a good choice for LG-XL dogs/power chewers!
Color: 3 PC-Beef-Red, Color: 3 PC-Beef-Red
Fantastic that these come in a 3 pack - I wish more chew toys did this! The base for these chew toys is pretty tough! The center & ends are hard enough to withstand both my bully mixes & my Irish wolfhound/Great Dane cross. They’re safe enough for even the largest power chewers! (With Supervision) my American Bully got the rubber spikes chewed off in under 20 minutes which is mildly disappointing but he’s a true powerhouse & master destroyer of all chews so I’m not that bent out of shape about it. I do wish they were as large as they appear in the ad pics but isn’t that the game we all play ordering offline anymore? 🤷🏻‍♀️ • Pro Tip: smear this toy in peanut butter (xylitol free!), wet food mixed into a slurry with water or bone broth + kibble/treats, yogurt, or even pumpkin puree and freeze for a few hours & relax while watching all the organic engagement your dog gets out of this toy!
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Reviewed in the United States on June 3, 2026

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