SKU: 19457208937

Human IL-4 High Sensitivity ELISA Kit

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Description

Human IL-4 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 4 existing in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.03pg/ml

Detection Range: 0.25-16 pg/ml

Recovery Range: 94-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.

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SKU: 19457208937

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44Magpie
Charlottesville, US
★★★★★ 5
Great World Time Watch!
Color: Stainless/ Black Dial
The usual quality Citizen construction, Eco-Drive, and a very handy world time bezel. FIVE STARS!
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Reviewed in the United States on February 8, 2026
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DS
Lexington, US
★★★★★ 4
Great watch for the money
Color: Stainless Mesh/ Blue Dial
Looks great. I swapped out the band for a leather strap. Nice watch, well designed. My other eco drive has been going strong for 15 years or so without a hitch. So I have high expectations for this one.
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Reviewed in the United States on January 9, 2024
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Gael Rivera
Natrona Heights, US
★★★★★ 5
Great Gift
Color: Stainless Mesh/ Blue Dial
It was a gift for my Dad but he loved it! Very worth it
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Reviewed in the United States on April 23, 2026
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Anthony
West Palm Beach, US
★★★★★ 5
Gotta have a citizen watch
Color: Stainless/ Black Dial
Fell in love with this watch..
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Reviewed in the United States on February 22, 2026
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Vincent Petralia
Chelsea, US
★★★★★ 1
Flimsy, small face
Color: Stainless Mesh/ Blue Dial
This watch doesn’t feel like the typical eco drive. The face is small, it’s very light and the mesh band was a pain to clasp. I returned it the day I got it.
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Reviewed in the United States on January 5, 2024

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