Human IL-31 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-31 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-31 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-31 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-31 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.78 pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 84-117%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 31 (IL-31)Yes α A short chain member of the helical cytokine family with a molecular weight of 24 kDa, which is mainly associated with activated T cells, is preferentially expressed by Th2 cells rather than Th1 cells. Human IL-31 cDNA encodes a 164 amino acid precursor, which includes a 23 amino acid signal peptide and a mature protein containing 141 amino acids. Human and mouse IL-31 share 24% amino acid sequence homology in the mature region. IL-31 generates signals through a receptor complex, which consists of IL-31 receptor A (IL-31Ra) and tumor suppressor M receptor subunits. These receptor subunits are expressed in activated monocytes and unstimulated epithelial cells. IL-31 signaling is involved in jak/stat pathway, PI3 kinase /akt signaling cascade and MAP kinase pathway. In addition, IL-31 plays a role in skin inflammation.