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Description
HRV 3C ProteaseProduct Specification Synonyms Protease 3CPreScission Protease Amino Acid Sequence Expression System E. coli Molecular Weight 21 kD (Reducing) Purity 95% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 50mM Tris HCl150mM NaCl10mM EDTA1mM DTT50%(v v) glycerolpH 8. 0 @ 25 Reconstitution Stability & Storage Store at 25 ~ 15 for 2 years Reference [1] Knott J A , Orr D C , Montgomery D S ,et al. The expression and
Product Specification
| Synonyms | Protease 3C、PreScission Protease |
| Amino Acid Sequence | / |
| Expression System | E.coli |
| Molecular Weight | 21 kD (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol，（pH 8.0 @ 25℃） |
| Reconstitution | / |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference |
[1] Knott J A , Orr D C , Montgomery D S ,et al.The expression and purification of human rhinovirus protease 3C.[J].European Journal of Biochemistry, 1989, 182(3):547-55. [2] Xu H , Wang Q , Zhang Z ,et al.A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease[J].Enzyme and Microbial Technology, 2019, 123:15-20. |
Background
Recombinant human rhinovirus(HRV)3C protease is widely used in the purification and productions of proteins due to its good stability，high specificity，and enzymatic activity．3C protease is one of the non—structural proteins of HRV，and plays important roles in the life cycle of HRV．This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.
Components
Storage Solution: 1U/μl 3C Protease50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol（pH 8.0 @ 25℃） 10*Reaction Buffer: 500mM Tris-HCl，1.5M NaCl，10mM EDTA，10mM DTT，（pH 7.5@ 25℃）
Protocol
1. Combine 100 μg of fusion protein, 10 μl of 10*Reaction Bufferr and H20 (if necessary) to make a 100 μl total reaction volume. 2. Add 1 µl HRV 3C Protease 3. Incubate reaction at 5°C for 16 hour.
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
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