Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Sep 6 - Sep 11
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse mTOR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the mechanistic target of rapamycin (mTOR). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of the mechanistic target of rapamycin (mTOR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse mechanistic target of rapamycin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | The mTOR target of rapamycin (mTOR), formerly known as the mammalian target of rapamycin and sometimes referred to as FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase encoded by the mTOR gene. mTOR associates with other proteins and functions as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, that regulate diverse cellular processes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.16-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 22 reviews
Sort
Product Reviews
★★★★★ 5
Made a big improvement in cabin noise—highly recommend
Model: SLH20025
Siless Hybrid made a huge difference in reducing noise in the floor of my truck cabin. In some areas, there was basically just bare sheet metal between the inside and outside, so adding this really helped the truck feel more solid and noticeably quieter on the road.
I used Kilmat on the doors, and honestly I wish I had used Siless Hybrid there too, but it was too late by the time I realized the difference. This material is very sticky, easy to cut, and simple to apply, which made the install pretty smooth.
Just a heads-up: the tacky side gets messy and will bond to clothing really easily, so be careful during installation. Overall, I’d definitely buy it again—great product with real results.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 2, 2025
★★★★★ 5
Very good product at a fair price
Model: SLH20025
Very good sound deadener and heat shield, it is a bit sticky to work with, but easy to shape and cut
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
★★★★★ 5
I wish I had Siless 20 years ago!!!
Model: SLH20025, Model: SLH20025
I have installed all kinds of insulation in vehicles over many years. I only needed to purchase 2 boxes to complete the entire interior siding of my 2025 chevy express, what a great value! I wanted to insulate my locksmith van for sound and heat. The sound reduction virtually eliminated the road noise. These panels also look amazing. It got up to 110F in the sun with the doors closed. After adding Siless insulation the temperature maxed out at 97F. That is a massive difference. Plus, I didn't even finish with the roof before testing. Siless Hybrid 3in1 was extremely easy to cut and adhere onto metal panels. I'm impressed with the quality of the individual panels and the adhesive is not toxic smelling compared to some others. My favorite thing about these panels is you can actually take them off and readjust them before they finally stick and they do not leave any black residue behind when you pull them up to adjust them. All I needed was a heat gun, knife and a roller to complete this job. The size of each panel is perfect, you can handle them with ease. I probably won't need your product again for a while, but when I do I will definitely be back.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 21, 2025
★★★★★ 5
Works well and is easy to install.
Model: SLH20025, Model: SLH20025
I was sound and heat insulating a generator bay in my class A RV so I did three tests before and during material application: I did a sound level test to see how effective the material was for my application, a "stickiness" test, and a flammability test. This was indoors on a dry 78°F day. From beginning to end, the entire process, including testing, took 5 hours to install 25 sqft or material in a very complex area. One hour for cleaning, 3 hours for installation, and 1 hour for test setup and testing.
Using a sound meter, I took before and after readings and determined that I reduced the sound level by 2.2 db (40% energy decrease) with a noticeable decrease in sound and frequency. The generator bay is located under the master bedroom in the aft portion of the coach. The sound went from a rattling, vibrating generator noise to a more tolerable low level rumble.
The material was easy to apply. I did some testing prior to application and determined that surface preparation is key. I cleaned my painted metal surface and then degreased with acetone and the material stuck well. Be careful and purposeful when you apply the material because once it was stuck to the metal, it was quite difficult to remove or reposition.
Since it was going to be installed into a gasoline powered generator bay located under our sleeping quarters, I did some flame testing to determine the level of risk. Although not highly flammable after installation, my testing revealed that the PE used will catch on fire under certain circumstances. In order to reduce the risk, I used aluminized duct tape to ensure that all the edges were sealed and the PE was not exposed to direct flame in the event of a fire. I also decided to install an automatic fire extinguisher as an added precaution. In all fairness there should be one installed in the generator bay anyway.
All in all, I am very satisfied with the product and the results. I have recommended this product to my other RV owner friends.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2025
★★★★★ 5
Great product. Best value i could find
Model: SLH20025
I'm using talk to text, so forgive any grammatical and spelling errors.
I ordered this product after doing a bit of research on sound dampening materials. I had been doing car stereo for quite some time since my teens. Itook a break for 20 years or so. I'm now 40 and back in the day there was dynamat. And that was about it. as irecently bought a new 2017 F350 crew cab. I wanted to put in a new stereo system and being that it was my dream truck. I was going to put in my dream stereo. But before I could do that, I needed to start with the sound deadening. I looked into today's leading product and found a company called resonx. Had taken their place as king of the hill in sound deadly. I researched their products and found the materials they were built from and Silas was the closest match at the best price I used Silas Max. First, then Silas 3 and 1 hybrid over that on all exterior panels. Then, on the interior panels. I used the 3 and 1 hybrid . on the floor I used Silas Max. Then, the 3 and 1 hybrid, then Silas liner over that the car is incredibly quiet . As far as sound dampening and deadening, it works very well for the money really can't beat it. Do your homework no How and where to apply it? And you really can't go wrong or about $400. I did. What would have cost me easily? Two thousand in other brands highly recommend the product prep work.Is everything do your homework?Do it right and you will be happy with this stuff
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2026