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Description
Human IL-12 P70 Onestep ELISA KitProduct Specification Antigen IL 12 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 3. 4% Inter assay: 5. 2% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 2. 875 pg mL Range 6. 25 pg mL 400 pg mL Recovery Cell culture supernatant: 97%
Product Specification
| Antigen | IL-12 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.4%； Inter-assay: 5.2% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.875 pg/mL |
| Range | 6.25 pg/mL – 400 pg/mL |
| Recovery | Cell culture supernatant: 97% Serum: 81% Plasma: 83% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
IL-12 is a key immunoregulatory cytokine produced primarily by immune cells like macrophages and dendritic cells. Its fundamental role is to bridge innate and adaptive immunity. It potently stimulates the activation of natural killer (NK) cells and T cells. A major function is driving the differentiation of naive T cells into the T-helper 1 (Th1) type. This action prompts T and NK cells to produce interferon-gamma (IFN-γ), a critical cytokine for cell-mediated immunity. Consequently, IL-12 is essential for defending against intracellular pathogens and tumors. It also enhances the cytotoxic activity of immune cells, enabling them to destroy infected or malignant targets. Due to its potent effects, its activity is tightly regulated within the body. Dysregulation of IL-12 is linked to autoimmune diseases, highlighting its powerful inflammatory potential. In summary, IL-12 is a master regulator of pro-inflammatory immune responses.
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