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Description
Mouse LGALS3BP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. 4. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Soluble galectin-3 binding protein, also known as LGALS3BP, is a protein encoded by the LGALS3BP gene. It is found at elevated levels in the sera of cancer patients and those infected with human immunodeficiency virus (HIV). It appears to be involved in immune responses related to natural killer (NK) and lymphokine-activated killer (LAK) cytotoxicity. It promotes integrin-mediated cell adhesion and may stimulate host defense against viruses and tumor cells. It regulates centriole biogenesis and centrosome hypertrophy in cancer cells. It has been shown to interact with LGALS3. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.0 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
Book Review of The Alchemist
Format: Paperback
In The Alchemist, Paulo Coelho uses diction, syntax, and the hero cycle to keep the pace of the book constant, and keep the reader intrigued. But, he does have a few flaws present in the book.
The Alchemist is a book about a boy named Santiago, who goes on an adventure to find his “Personal Legend” or treasure. Coelho uses the hero cycle to add more emphasis on the plot, which overall makes the story more entertaining. In the story, the Old King tells Santiago that “when someone wants to realize his or her Personal Legend,” he will “always [be] nearby.” (67) This is an example of atonement with the father, because the Old King’s closeness creates a father like figure in him, who is there to support Santiago. The hero cycle for me made the story more enjoyable and kept me reading. Also most of the book is him trying to obtain his Personal Legend, which is “what you have always wanted to accomplish.” (23)
Compared to other books I have read this year in my English class, this one was easier to follow in terms of diction. The language that Paulo Coelho uses in the novel overall is not too difficult to understand. Word choice is done very well, and lets the reader flow through the book at a constant pace. The diction also makes the book a novel for a wide range of readers. The hero cycle with the clean diction made the story very enjoyable.
While reading this book, I found that it was fairly easy to understand the passage, and that it was very inviting. Paulo Coelho structures the syntax of the book in an easy-to-understand format. Throughout the book, the syntax helps the reader understand where they are on the page, and it also positively affects the pace set by the diction in the book. Towards the middle of the novel, the boy says that “An army is coming,” and he knows because he “had a vision.” This dialogue is quickly followed up by a response from a camel driver, saying that “the desert fills men’s hearts with visions.” (104) The quick pace of dialogue keeps the book driving keeps the pace constantly in motion. The syntax made it easier for me to follow the story and follow which character was speaking and what they where feeling or doing.
Even though I enjoyed a great bit of the book, there was still another bit that I didn’t fully enjoy. At the beginning of the Alchemist, the book seems to go a slower speed then the rest of it. The first portion of the book has a lot to do with introducing Santiago, the plot, the setting, and more. There is a part in the story where it goes from page 35 to page 38 with very little dialogue, which I felt stopped progress of the story. The first part also has to do a lot with how he discovers his Personal Legend. It took me a little while longer to read the first part of the book then the rest because I was mostly bored while reading it. I also felt that there was more descriptive text than the rest of the book, which really dragged out the first part.
In conclusion, the book has lots of great traits and only a few bad ones. I would definitely recommend this book to a friend or anyone who enjoys reading about adventure.
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Reviewed in the United States on January 12, 2016
★★★★★ 5
such an adventure
Format: Kindle
Loved this!! Lots of questions to ponder and the simplicity of it all 🫶🏻. A short read but so very profound
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Reviewed in the United States on May 22, 2026
★★★★★ 4
Interesting story
Format: Paperback
The Alchemist is an engaging modern fable centered on self-discovery, personal destiny, and following your dreams. It offers interesting life lessons and an easy-to-read story. While its themes draw on various spiritual ideas and may feel unconventional, it still delivers meaningful reflections on purpose and perseverance. Overall, a thought-provoking and enjoyable read.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
One Of The Most Important Books Ever Written
Format: Paperback
The Alchemist by Paulo Coelho is in my opinion, one of the most important books ever written. It's no wonder that it's one of the best-selling books of all time with more than 150 million copies sold. Those numbers are in spite of and defiance to the author's encouragement to file share his now legendary novel.
But sales statistics are not the criterion on which I stake this claim. It is the impact on the lives of those that have read it, and the impact on the lives that have not yet been born, that I boldly state the above.
The simplicity of its unfoldment may at first belie the depth of its wisdom. But the simplicity is part of the profundity that allows the reader to assimilate the messages without resistance.
The first time I read The Alchemist;, I read it cover to cover in one sitting. No bathroom break, no cup of water pause, no telephone distractions. I read it from the first word to the last in what seemed like a single breath.
I simply couldn't put it down.
I was swept, swung, and swallowed up by this extraordinary narrative. The result was transformation. I was not the same man that began reading the novel by the time I finished it.
I would thereafter read it again and again. The messages are so layered that with every new pass comes new understanding and enlightenment.
At the core of the novel is the fulfillment of one’s destiny. There is a force in the Soul of the World that wants us all to live out our personal legend. When we truly desire something, the entire Universe conspires to help us achieve it. With love, all things are possible. With love, we can perform miracles.
These themes are artfully integrated and clothed within a modern day fable. They come in varying iterations from a string of colorful characters that propel the protagonist towards a profound and satisfying ending.
The Alchemist is the story of Santiago, a shepherd boy from Spain that crosses the desert of Africa in search of a hidden treasure. It was revealed to him in a reoccurring dream that the treasure is buried by the Ancient Pyramids in Egypt.
What he naively believes will take three weeks to complete becomes a journey that expands nearly three years. After miles of traveling through the desert, the boy arrives at an oasis where he falls in love with a beautiful desert woman named Fatima. The boy is willing to abandon his dream of finding his treasure for the procurement of her love.
But one night, beneath the light of a full moon, he meets a man straddled upon a mighty horse. This elusive man is known in legend and folklore as The Alchemist.
The Alchemist is a man imbued with profound wisdom and mystical powers. He is reportedly over 300-years-old and can turn lead into gold.
He reminds the boy that his destiny is to find the treasure. That he must continue. If it is true love, Fatima will be waiting for him after his destiny is fulfilled.
Reluctantly, the boy continues his journey deeper into the desert accompanied by The Alchemist. But the desert is at war, and they are soon captured by Bedouin sentinels and accused of spying for the enemy.
For this crime, they are sentenced to death.
The Alchemist saves them temporarily by proclaiming that the boy is an Alchemist. He tells the general that if he wanted to, the boy could turn himself into the wind and destroy their entire camp. The general is intrigued! He wants to see this happen. He promises to spare their lives if it is done. He grants them three days.
But how does a shepherd boy from Spain turn himself into the wind?
Paulo Coelho says that he wrote “The Alchemist” in two weeks. It is almost hard to imagine that something so far-reaching could have been conjured up in two weeks time. He said it was his own journey that he was writing down, parabolically speaking.
The impact of “The Alchemist” comes from the realization that Santiago’s journey is Everyman’s journey. His story is our story. If we tap into the Soul of the World and dare to live out our destiny, we will discover that within us all is The Alchemist.
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Reviewed in the United States on April 21, 2017
★★★★★ 5
A must read
Format: Kindle
A must read for everyone even remotely interested in self fulfilment and awakening. Can be read in an afternoon and echo for a life time.
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Reviewed in the United States on May 25, 2026