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Description
Human HSF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Heat Shock Transcription Factor 1 (HSF1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Heat Shock Transcription Factor 1 (HSF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Heat Shock Transcription Factor 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Heat shock transcription factor 1 (HSF1) is a protein encoded by the HSF1 gene. Highly conserved across eukaryotes, HSF1 is a major mediator of transcriptional responses to proteotoxic stress and plays important roles in non-stress regulation, such as development and metabolism. Diseases associated with HSF1 include heat shock RNA 1 and prion disease. Pathways associated with HSF1 include cellular responses to heat stress and ERK signaling. Gene Ontology (GO) annotations associated with this gene include DNA-bound transcription factor activity and chromatin binding. An important homologue of this gene is HSF4. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Functional + Sexy
Size: Small, Color: Multicoloured E-(5-pack), Size: Small, Color: Multicoloured E-(5-pack)
Not only do these feel good, my husband can’t keep his hands off me. They fit so good and are soft. I change into these to sleep but also just use them as shorts to wear around the house. They’re comfortable and don’t slide around. I got a size small and I’m 100lbs.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2026
★★★★★ 5
Finally!
Size: X-Large, Color: All Black D (5-pack)
Finally, comfortable underwear that don’t end up giving me a wedgie. I have a round booty and most cuts of underwear end up shifting as I walk and sit. The hem of the underwear comes right under my cheeks and the lightweight material ensures there’s no panty line and no shifting to where I don’t want them! I’m definitely purchasing more.
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Reviewed in the United States on May 17, 2026
★★★★★ 3
Ehh
Size: Medium, Color: Multicoloured E-(5-pack)
Very thin, still comfy but very thin material. Super soft though. I've worn and washed and they have held up but I won't be repurchasing bc these are so thin
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Reviewed in the United States on April 14, 2026
★★★★★ 5
They’re fantastic!!!!
Size: X-Large, Color: Multicoloured E-(5-pack)
My clothing size is 16/18 and XL. Even though Amazon suggested a size XXL, I ordered my normal XL and they fit perfectly.
The fabric for this style is 92% bamboo viscose and is amazingly soft and almost silky feeling. I had ordered a different boy short in this brand but they were 80% bamboo viscose and the fabric was significantly heavier. I returned those and ordered these.
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Reviewed in the United States on April 25, 2026
★★★★★ 5
Boy have I bought A Lot of Underwear
Size: Medium, Color: Black,navy,melange,london Blue,burgandy
These are my favorite boyshorts so far. The little stripes kill me, they're so cute. I am now an expert on boyshorts. You should know, I always buy the color packs with boyshorts with a make-up that look like static on the tv. Not sure how else to describe that but they tend to be the same across the board and I think they're super cute, personally. The solid colors may be a different story. SO-Here's what I've learned: They're all basically the same, minus the material and how you like them to fit. You want to look at what the exact material content is. Do you like super soft, almost gauzy and you're not concerned with possibly snagging them? Then you'll want to find boyshorts with a high percentage of polyamide. These are not them. These are about 50/50 polyamide and polyester, which is firmer and has less stretch. Not gonna snag them in regualr situations. Still lots of stretch, though. Just more support and more excercisey material feel. But, there is enough polyamide in them to still feel soft. I prefer less plush and more support. There is a dash of spandex in there and that's good to accommodate many bodies and to hold their shape over time. I know some gals are looking for a softer, stretchier, plushy boyshort. They're easy to find. Look at the material listed. Okay, about sizing: These are stretchy. Not SUPER stretchy, like the gauzier underwear, but stretchy, and you'll fit in most of the sizes. The fit will just be different, obviously. I'm a large in regular pants typically. Size 12. Ten in a good month. I'm 48, 5'5 and 165 pounds. I've got a booty, but not super-duper bangin. Just big. Anyways, I've gotten my "true" size (L), sized up (XL) and sized down (M). Sizing up (XL) is too "poochy" feeling and just mildy wads up around where my legs meet my bits. Comfortable, and stay on but saggy feeling to me. The true-to-size, size large, are less "waddy" and fit fine! Loose enough to almost forget they're on. I've sized down to medium, and that's where my preference is. They fit like I have work out shorts on, just very short ones. More support but don't look ridiculous. I don't look like a sausage in them. Size small would be that on me-the sausage shorts. I mean, I'm wearing these around the house in the summer so I don't have to crank my A/C., and for bed. They aren't gonna be seen my many. Either way these are going to roll up (super skinny gals without thigh gap...do boyshorts roll up on you too? Always wondered that) but the smaller size on me seems to roll less, and that roll doesn't bother me. A roll is different from a "wad". Actually I wouldn't even call it a roll, I'd call it a "fold over". Ha ha I hope this helps and you understand my lingo. FYI I have found personally that most of these brands are the same exact thing from China, packaged and branded differently. The material is the big factor. But these are the only ones I've found with little pinstripes. CUUUTE. Good luck out there!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 17, 2025
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