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Description
Human FAM3D ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against sequence similar family 3 member D (FAM3D). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of sequence similar family 3 member D (FAM3D) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Sequence similar family 3 member D ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Family of sequence similarity 3, member D (FAM3D), a member of the FAM3 family of cytokines, possesses a four-helix bundle structure. 11 FAM3D is constitutively expressed in the gastrointestinal tract, and its expression and production are regulated by nutritional factors. Specifically, FAM3D is upregulated in humans after consuming a high-fat diet. FAM3D upregulates and activates Mac-1 (macrophage antigen-1) in neutrophils, and inhibition of FPR1 (formyl peptide receptor 1) or FPR2 significantly blocks FAM3D-induced Mac-1 activation, indicating that the effects of FAM3D are dependent on these two FPRs. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Jelly Star mini smartphone is a bundle of joy
Color: Blue, Color: Blue
I've been using the Jelly Star about a week now and have no regrets about my purchase. I have the blue color and it's really pretty. This phone is as good as the reviews I researched said it was. Very fast, has all features of a regular smart phone but easy to hold in one hand and carry in pocket. This phone is 3/4" thick with a screen 2.75" x 1.5" and comes with a protective case. Good connection with T-Mobile cell towers, nice variety of ring tones, decent camera for snapshots. When it first arrived I was hesitant to commit, worried that with the tiny print I would struggle, but now that I got it connected I'm really happy with it. I'm old enough that without reading glasses, I need to squint to see the print in text messages, but it has a zoom feature that works well once you get used to it, and I plan to use other devices for viewing the images I take with it. Bought it mainly to have as a basic communication device that isn't bulky/in the way all the time. Don't have to constantly charge the battery either. Only using the phone for calls, texts, and WhatsApp; so far only need to charge it every 3 days. I took the time to research online how to stop apps from running in the background which should also help conserve the battery. I'm looking forward to loading music on it and maybe even taking it for a run once I get the headphones and armband accessories. I recommend it.
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Reviewed in the United States on May 4, 2026
★★★★★ 1
Screen Stopped Working Amazon Denied Refund
Color: Black
I bought this phone 2 months ago and within a week the screen stopped working. It stopped responding to any touch. I sent it back to Amazon in its original physical condition in its original packaging and ordered a second one. The second one has battery issues where it randomly starts heating up and dies within a couple of hours. Just found out amazon denied my return because they said the phone was damaged. It was not. I love the idea of the phone but the execution is poor and because there's no reliable way to get replacements I definitely would not recommend risking it.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Works on Visible (Verizon)
Color: Black
For the price, this little device offers no compromises aside from the obvious. It's quick, offers a full day of battery life, and has a feature set to rival much more expensive competition. The screen despite it's size is fully usable for my every day tasks. Texting, web browsing, reading comics, etc are all viable if you have the eyes for it. The build quality is great. This reminds me of an old Nokia in the hand. I've also had no signal problems in rural Appalachia.
And yes, it *does* work on Verizon, but you'll need another phone to activate the sim with before transferring it over.
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Reviewed in the United States on March 11, 2026
★★★★★ 5
The phone work is compatible with MetroPCS by T-Mobile or Verizon
Color: Black
It seems to be a very good quality the compatibility is just like a larger phone and it seems to be durable and compatible the size it's all right yes I would recommend other people use it or buy it because it's really not an inconvenient item
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Reviewed in the United States on April 15, 2026
★★★★★ 5
Cut your screen time without sacrificing convenience
Color: Blue
I've been wanting to switch to a "Dumb" phone to cut back on my screen time, but I was nervous about needing navigation and the internet away from home.
Well! The Jelly Star is a fantastic solution.
Cons:
-Obviously, typing can be quite difficult. But the audio transcription features work very well.
-The small screen means that every small finger gesture can trigger an unintended action
Pros:
-No loss of function. This phone can do 98% of anything a full size phone can do.
-Cut screen time immediately
I read a lot about this phone, but still wasn't sure what it could handle. My main goal was to cut screen time so I took a shot on it.
Right out of the box I was surprised at how "normal" it was in every way but the size and shape. I was able to still use all of the apps I used on my old phone, I could set up my home screen with widgets I had before, and download the same custom ringtones. Even the youtube popup viewing window, screen recording, messenger! It feels exactly the same to use as any other android phone, just incredibly small.
The camera, audio, fingerprint scanner and microphone are all meh, but completely acceptable for a fully functional smartphone that cost $200.
Now, did it help with screen time? YES! Right away I really lost the desire to get "stuck" watching videos. The phone doesn't take up much of your field of vision; its hard to get sucked in and not be immediately distracted by the real world outside your screen. And as it was intended, the small phone screen (while serviceable in every way), just isn't much fun to be on.
Typing on this phone is a bit slow and methodic, I make more phone calls and speak to people in my life a bit more regularly.
And of course, I still crave entertainment but I have been choosing more audio books, music and podcasts over scrolling on tiktok/instagram/facebook.
Having only had this phone a short time, I am blown away by how both effective and usable it is! If you want to cut down on screen time but not give up the convenience of a smart phone, this is the phone for you!
For the price it comes with a screen protector (Applied), and extra screen protector, case, cable, brick, and a strap! It comes fully protected out of the box.
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Reviewed in the United States on January 12, 2026