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Description
Human 17β-HSD3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a 17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of 17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human 17β-Hydroxysteroid dehydrogenase 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | 17-β-Hydroxysteroid dehydrogenase 3, also known as HSD17b3, is an enzyme encoded by the HSD17b3 gene. It is involved in androgenic steroidogenesis. This 17β-HSD isoform is primarily expressed in the testes and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as a cofactor. Deficiency can lead to impaired masculinization in hereditary male infants, formerly known as male pseudohermaphroditism. Related disorders include 17-β-Hydroxysteroid dehydrogenase III deficiency and pseudohermaphroditism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Not greasy at all
Size: 6 Fl Oz (Pack of 2)
This is my first mineral sunscreen. I love that it is not greasy at all. It's easy to apply, and hasn't caused any irritation to my baby's skin.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2025
★★★★★ 5
Fantastic start up
Truskin products are fantastic in my opinion. Not expensive AND effective.. what more can you ask. This 3 pack with Hyaluronic acid, Retinol, and Vitamin C is perfect. I go through the HA faster than the rest so I end up buying the bigger bottle of it. The Vitamin C serum feels incredible on my skin. It feels more moisturizing than the HA. I love using it- just please don’t forget the SPF. This combo pack is perfect for anyone looking to care for their skin. I don’t notice any off putting odors. They all dry down quickly. My skin has not reacted poorly to any of the products. I do stick to my routine of actives(retinol, vit c, tea tree- rotated)to 3x a week and the other days are gentle cleansing, moisturizer and SPF. Since I have switched my skin care routine to TruSkin, I have noticed better looking skin. I’m moisturized with soft youthful looking skin. Recommend and will be buying more.
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Reviewed in the United States on April 5, 2026
★★★★★ 5
I didn’t expect this to actually work.
I didn’t take a before photo. Because I legit didn’t expect this to do anything to my skin. I’ve used similar serums before with no results. But here I am, spreading the word because I’m a believer.
To describe my skin: aging (mid 40’s), a few wrinkles here and there, nothing insane, very fair, super dry, with an oddly greasy forehead. Minor sun damage, uneven texture and big fat pores that I loathe. Occasional zit here and there. I also have rosacea. It’s the kind of rosacea that turns me bright red when I’m overheated or angry, or if I rub my face too much. It’s doesn’t change the texture or anything, just turns me bright red. My dermatologist prescribed me a cream for it, but warned me that it is the kind of rosacea that is not always easy to treat because it comes from deep under the skin and is situational. The cream was short lived because it dried me out severely.
I recently had a trusted friend who is also an oculoplastics surgeon suggest a good vitamin c serum and retinol. He didn’t suggest a brand, just use of the product. So I went hunting on Amazon. This product had great reviews. I figured I’d invest in the three pack since I also knew Hyaluronic acid was a must.
My first impressions: Shortly after I ordered it, hadn’t yet received, I got an email from the company offering assistance if needed and gratitude for purchasing product. Good to know I had that standing by if needed. Who doesn’t appreciate a business committed to having happy customers?
My first impression for my first uses of the products was positive. Obviously it was too early to see any noticeable changes, but I did like that the serums don’t have smells, they dried very quickly and they’re super easy to rub in. A little goes a long way too. I’m going to reiterate that they don’t smell. Many products say ‘unscented’ but you can still smell something, bad or good. The only one I can mildly smell is the hyaluronic acid and it smells pleasant and clean, but even then, I can barely smell it when I first start rubbing it in. Any smell is gone almost instantly. I can’t describe the smell because by the time my brain realizes I’m smelling something, the smell is already gone. So that’s nice!
My impression now, after using it a while: I love it! My redness is greatly reduced. In addition to my rosacea, my very fair skin can be somewhat translucent in places and kind of reveals the semblance of some blood vessels under my skin. This has minimized them tremendously. My “elevens” are lessened as well. This was a big surprise…that wrinkle, along with some wrinkling around my eyes has noticeably lessened. And I want to reiterate that this is all easily noticeable to me without a before photo. It is hard to notice subtle, gradual changes when we don’t have a photo for before and after comparisons - but I’ve noticed. I introduced another item from another brand, and I think that it’s has helped greatly with my pores but I believe these serums have helped too- my pores are barely visible. I am also very impressed by the texture of my skin. I have ALWAYS struggled with uneven texture. I’ve done all the texture improving techniques, tried the products claiming to improve texture, everything I knew to do, with no real difference. But this- this changed the feel of my facial skin entirely. I bumped my own cheek a couple days ago and actually had to rub my face again to ensure it was MY skin I was feeling. It was like a tactile double take. Why? Because my skin was silky smooth. My makeup wears so much better on my skin. My skin looks noticeably younger and brighter and I haven’t had any new zits either since I started. I cannot express enough how happy I am with these changes and these serums. I truly expected these to be items I ended up not using and simply giving them away. But these are staples now. They are worth every penny and a bargain for the skin changes. I look forward to seeing more improvements in my skin over time and I highly recommend this product to any skeptic like I was.
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Reviewed in the United States on November 12, 2025
★★★★★ 5
Great stuff definitely recommend at least trying
I've been using the TruSkin Vitamin C serum for over a year, and it's done a solid job brightening my complexion and fading dark spots. Finally gave the full Face Serum Trio a shot with the hyaluronic acid and retinol, and after about a month, it's working great, just like the Vitamin C. The hyaluronic acid hydrates my skin well without any grease, and the retinol's knocked down some fine lines, making things smoother and fresher. Layering them has improved the texture and glow noticeably. No irritation on my sensitive skin. Solid value—I picked this up at $26 when it was Prime Day, but even at that $40 price range, it's still worth it in my opinion since it can last a while. Definitely recommend
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Reviewed in the United States on November 1, 2025
★★★★★ 4
Great deal, my face loves them
Love these and got them on a great deal! The retinol and vitamin c my face loves!! The hylalonic acid seems super similar to the vitamin c in formula, I use that one less.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 9, 2026
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