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Description
Human KLK6 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. CSF breast milkOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Kallikrein 6 (KLK6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Kallikrein 6 (KLK6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Kallikrein 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Kallikrein 6 (KLK6) is a protein encoded by the KLK6 gene. KLK6 is abundantly expressed in the central nervous system, with highest levels in the brainstem and spinal cord. Following central nervous system injury, including glutamate-mediated excitotoxicity, inflammation, or trauma, KLK6 is elevated, including at least transient increases in expression in neurons and oligodendrocytes, as well as in reactive astrocytes and microglia. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cerebrospinal fluid, breast milk, and other biological fluids |
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4.5 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Works better than more expensive ones
Color: Pink
This shaver surprisingly works really well. I don't really have hair long enough to use an electric shaver, but the other end, the foil type shaver works really well, even on very short hair. It left my skin actually smooth, which is better than most of these types of shavers, which leave it close to smooth but actually kind of just really short, so you can feel it even if you can't see it. This felt much smoother than other shavers I've tried. I like that there's less skin damage than a razor. It's smaller than is shown in the photos, which is actually good because it's better for travel. In the photos, it looks comically large, but it just looks like a normal pink shaver in person. It's actually a good size for every day use. It would probably be okay for travel, but it's definitely not mini. It would probably be good for keeping an undercut fresher. The price, at $30, is lower than many other brands that I've seen/tried. I've tried several with $50 price tags that are only the foil end and didn't work as well as this one, and we're half the shaver this one is. At least on first impression, this is a good quality and decent value. Hopefully, it stands the test of time.
Update: I was not planning on using it for this, but my very fat and old cat gets the occasional knot in the spring when he's shedding, and OMG, this is the best shaver I've used on a cat. It glides like butter to skin short. It takes a literal second. This thing is amazing.
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Reviewed in the United States on May 13, 2026
★★★★★ 1
Buyer Beware
Color: Blue
Product comes with no instructions on how to operate it , just a small enclosure telling you how to disassemble it. Mine would not work even after I charged it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
★★★★★ 4
Lightweight and Easy to Use - A Solid Option for Occasional Trimming
Color: Pink
I received this trimmer through the Amazon Vine program in exchange for an honest review. I was drawn to it partly because of the dual-head design - having both a trimmer and a shaver head in one device seemed useful. I also noticed that it was marketed for sensitive skin, which I thought was important given the sensitive area this is intended for.
My first impression was that it's pretty lightweight and feels comfortable to hold. The pink plastic color looks decent enough, and while lightweight, the build quality seems solid enough for the price point. One of the first things I noticed when I turned it on was the LED light near the head - I wasn't sure how useful that would actually be going in, but it turns out it is actually helpful for visibility in hard-to-see areas. The digital display showing the battery percentage is also a nice to have, so you at least have some warning about when to charge it.
The device can be used both wet and dry, which adds flexibility.The main shaver blade is gentle - no redness or irritation. It does trim efficiently and moves through hair evenly. I will say it's not completely silent, and like other trimmers it may leave a little more behind compared to a manual razor. But for general use and maintenance, it does the job well.
Overall, I've been pleased with how it performs so far. It's lightweight, easy to handle, gentle on the skin, and the added features like the LED light and digital battery display feel like genuine usability improvements rather than just marketing extras. If you're looking for a straightforward, no-fuss trimmer for sensitive skin and occasional or regular use, this is a decent choice at this price. It covers a lot of different areas - bikini line, underarms, legs, face - so it's versatile enough to replace other tools you may have.
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Reviewed in the United States on June 5, 2026
★★★★★ 5
I bought it out of curiosity, and now it’s part of my weekly routine
After seeing so many electric shavers for women online, I decided to try this one because I liked that it came with multiple attachments and claimed to be gentle on sensitive skin. Honestly, I expected it to be just another grooming gadget, but it ended up surprising me in the best way.
The first thing I noticed was how lightweight and comfortable it feels to hold. Right out of the box, it looked elegant and well-designed, which I personally appreciate because I love products that are both practical and aesthetically pleasing. Plus, the different attachments are actually useful and not just included for decoration.
I first tested it on my legs and was genuinely surprised by how smooth and gentle it felt. No pulling, no irritation, and that alone was a huge win for me because my skin usually reacts easily after shaving. I later used it on my underarms and bikini area, and I really liked how delicate and comfortable it felt on sensitive areas.
One feature that made my routine so much easier is that I can use it both dry and in the shower. Some days I’m in a rush and can quickly do touch-ups before heading out. I also love that it’s cordless because it makes everything more convenient and easy to use.
The battery life is actually impressive, and the LED display is super helpful for knowing when it needs charging.
I feel like products like this make personal care routines simpler and less stressful. It’s obviously not the same as a spa treatment, but for everyday grooming it truly does a great job and leaves my skin feeling smooth without the hassle of traditional razors. Definitely one of those purchases that ended up being more worth it than I expected.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Easy to hold on to and shaves great
This shaver is of good quality. I like that the feel is kind of rubbery so it's easy to hold on to, it is small and easy to use. It charges up quickly also.
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Reviewed in the United States on June 6, 2026
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