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Description
Human VIL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a villin 1 (VIL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of villin 1 (VIL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Villin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Villin 1 (VIL), also known as VIL1, is a protein encoded by the VIL1 gene. This gene encodes a member of the calcium-regulated actin-binding protein family. This protein represents a major component of the brush-border cytoskeleton, playing a role in the capping, severing, and bundling of actin filaments. Two mRNAs, 2.7 kb and 3.5 kb, have been observed, each utilizing an alternate polyadenylation signal present in the terminal exons. It plays a role in actin nucleation, actin filament bundle assembly, and actin filament capping and severing. It regulates intestinal epithelial cell morphology, cell invasion, cell migration, and apoptosis. It protects against dextran sodium sulfate (DSS)-induced apoptosis in gastrointestinal epithelia. It appears to regulate cell death by maintaining mitochondrial integrity. It enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis, and wound repair. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.6 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Beautiful Acacia Boards That Actually Feel Premium and Thoughtfully Made
I’ve gone through a lot of wooden cutting boards over the years, and these immediately stood out as feeling much higher quality than most sets in this price range. The acacia wood has a really rich, attractive grain pattern and the boards arrived smooth, solid, and well finished with no rough spots, cracks, or cheap “composite wood” feel.
What initially caught my attention was the fact that these are marketed as non-toxic and free from mineral oil. Most people don’t think much about what wooden kitchen products are treated with, but I appreciated that these use a natural blend instead of petroleum-based mineral oil. The boards had a very clean, natural wood smell out of the box instead of that chemical/oily smell some cheaper boards have.
The three-size setup is honestly perfect for daily kitchen use. I find myself constantly rotating between them:
the large board for meal prep and meats
the medium board for vegetables
the small one for fruit, cheese, garlic, herbs, etc.
The engraved food icons are also a surprisingly nice touch because they help keep things organized and reduce cross contamination without looking tacky.
A few other things I really liked:
They’re lightweight enough to move around easily but still feel sturdy
- The wood is gentle on knives
- The groove catches juices well
- They look nice enough to double as serving boards
- Packaging was clean and plastic-free/minimal
Like any real wood cutting board, you absolutely need to:
- hand wash only
- dry them after use
- occasionally recondition the wood
If you expect dishwasher-safe convenience, these probably aren’t for you. But if you want attractive, natural wood boards that feel healthier and more intentional than cheap plastic boards, these are excellent.
Overall, these feel like a genuinely thoughtful kitchen product rather than generic mass-produced boards with a trendy “eco” label slapped on them. Very happy with them so far.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Beautiful in appearance and quality
I've been using these for several months and I love them. They're sturdy and great quality, not to mention so beautiful. They do of course scratch up as you use your knife on them which is to be expected with wood but I plan to give them a little sanding soon and an oiling with the oil they provided. I would definitely recommend.
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Reviewed in the United States on January 7, 2026
★★★★★ 5
Ignore the bad reviews, read mine!
Pictures don’t make you think much, but in-person these are beautiful and well made. I’ve been trying to replace everything with cleaner safer alternatives. Honestly I love everything this brand offers and stands for! Some things to think about when you see other peoples negative reviews. Since these cutting boards are all around safe and clean with no toxic coating’s that most have to make them last longer, they do require more maintenance, when people don’t follow that maintenance you get a warped or cracked board. Also they are not meant to last as long as your plastic one from Walmart, they should be replaced more often. Luckily for a pack of 3, and I’ll say again that are TRULY non toxic, they are super reasonably priced! For what they offer I would even pay more for these. They do also come with instructions on how to care for them! So if you are the type of person to leave things dirty, or wet in the sink, these are probably not for you. For anyone who doesn’t mind taking the extra minute these are a must (literally a quick wipe, rinse and hand dry, it’s nothing). Gives me a breathe of fresh air to know I’m using safe, and clean products.
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Reviewed in the United States on May 4, 2026
★★★★★ 1
Cutting boards will splinter
A lot of the reviews that are saying these wooden boards splinter are true. I was excited to find some nontoxic wooden cutting boards for such a great price. Upon arrival, they seemed pretty decent quality when I pulled them out of the package. I proceeded to use the large one so I gave it a quick rinse with warm water and was taken back by fumes coming off the cutting board and then wiped it down with a white paper towel and my paper towel was brown. I was a little discouraged, but decided to give it a better rinse with a soap, free brush. At that point once I dried it off whatever coding was on it came off and it was rough and splinters everywhere. Definitely a little upset that the splinters could’ve ended up in our food. These are not good quality non-toxic cutting boards I would rather spend a little extra money on a quality board that is not going to sprinter Wood shavings into my food.
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Very good, just be sure not to leave in water
Overall, this is a very good product as my wife and I are moving towards non-toxic kitchen materials. My only advice is to make sure to not leave this submerged in water otherwise mold will easily form.
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Reviewed in the United States on December 27, 2025
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