SKU: 87704745858

Furin/PCSK3 His Tag Protein, Human

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Description

Furin/PCSK3 His Tag Protein, HumanProduct Specification Species Human Synonyms FUR,PCSK3,SPC1,PACE Accession P09958 1 Amino Acid Sequence Gln27 Ala574 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 55 72kDa (Reducing) Purity 90% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Human
Synonyms FUR,PCSK3,SPC1,PACE
Accession P09958-1
Amino Acid Sequence

Gln27-Ala574 with His Tag at the C-Terminus

Expression System HEK293
Molecular Weight

55-72kDa (Reducing)

Purity >90% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose

Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· Please do not repeated freeze-thaw cycles.

Reference

1.Anderson ED, Molloy SS, Jean F, Fei H, Shimamura S, Thomas G. The ordered and compartment-specfific autoproteolytic removal of the furin intramolecular chaperone is required for enzyme activation. J Biol Chem. 2002 Apr 12;277(15):12879-90.
2.Dahms SO, Hardes K, Becker GL, Steinmetzer T, Brandstetter H, Than ME. X-ray structures of human furin in complex with competitive inhibitors. ACS Chem Biol. 2014 May 16;9(5):1113-8.

Background

Furin, also known as Paired Basic Amino Acid Cleaving Enzyme (PACE), is a key member of the subtilisin-like proprotein convertase family. Functioning as a calcium-dependent serine protease, its structure includes an N-terminal signal peptide, a prodomain, a catalytic domain, a P-domain responsible for maintaining enzymatic activity and calcium/pH sensitivity, a transmembrane region, and a cytoplasmic tail. Primarily localized within the Golgi apparatus/endosomal system, it activates a wide range of proprotein substrates—such as growth factors, receptors, extracellular matrix proteins, and pathogen proteins (e.g., envelope proteins of HIV and influenza virus)—by specifically cleaving after the Arg-X-(Arg/Lys)-Arg sequence motif. It plays a critical role in embryogenesis, peripheral immune tolerance, and cellular homeostasis. Dysregulation of its function is closely linked to tumor progression, neurodegenerative diseases, and various pathogen infections, making it a significant therapeutic target in clinical applications.

Protocol

Assay protocol
Principle: Measured by its ability to cleave the fluorogenic peptide substrate, Pyr-Arg-Thr-Lys-Arg-AMC.
Materials
Assay buffer: 25 mM Tris, 1 mM CaCl2, pH 9.0
Furin/PCSK3 His Tag Protein, Human (UA011272)
Substrate: Pyr-Arg-Thr-Lys-Arg-AMC (abs45133051)
96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
Plate Reader (PerkinElmer, excitation 380 nm and emission 460 nm)
Produce
Dilute Human Furin Protein to 2 µg/mL/4 µg/mL/8 µg/mL in Assay Buffer.
Dilute Substrate to 100 µM in Assay Buffer.
Load into a black well plate 50 µL of 2 µg/mL/4 µg/mL/8 µg/mL of Human Furin Protein, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 10 minutes.
Calculate specific activity:
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino-4-Methyl Coumarin
Conversion Factor (pmol/RFU)
Produce
Buffer: Assay buffer
Calibration standard: standard 7-amino-4-Methyl Coumarin (Enzo, Catalog # BML-KI107-0001).
Add 100 µL Dilute Calibration standard, Standard curve 1500/750/375/188/94/47/24/12/6/3 pmol per well or blank (Assay buffer) per well.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in endpoint mode.
Linear Regression of 7-amino-4-Methyl Coumarin (pmol) (y)—RFU-Blank(x).
Note
Storage Conditions: After reconstitution, aliquot and store at -80°C. Please do not
repeated freeze-thaw cycles.

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SKU: 87704745858

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Alp acct
Louisville, US
★★★★★ 4
doesn't whistle as loud as other chuckit balls I've seen, but a good ball.
makes some noise, but I've seen (and played with) others at the dog park that whistle much louder than the 1 I got. all Chuckit balls... so, just this one I'm betting. but it flys good and the dog loves to chase it. he chews it a fair bit as well and the ball has not taken much damage from him (lab mix) or others that have had a go at it. I'll likely buy another at some point ... just wish it was a little louder when flying.
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Reviewed in the United States on November 4, 2025
K
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KA
Boise, US
★★★★★ 5
Blue ball, my dog likes blue.
Just a ball right? Evidently not! My dog won’t chase an orange chuck it ball or the glow in the dark ball- only the blue one 🤷🏼‍♀️
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Reviewed in the United States on May 9, 2025
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Baker-s
Fort Morgan, US
★★★★★ 3
I wanted a blue ball
It's great. It just wasn't blue.
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Reviewed in the United States on January 5, 2026
J
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Jr
Omaha, US
★★★★★ 5
its a great ball
works well
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Reviewed in the United States on July 8, 2025
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Tabs
Boise, US
★★★★★ 5
Love this set!!!
Size: Medium (2.5"), Style: Fetch Pack 1
I was trying to figure out which of the many different balls this company offers, and I was getting frustrated but then i was SOOOO happy to see they had multiple sets that had multiple of the different balls they offer!! I bought this set and the other set they offer, but this one was my favorite and I think my dogs too! The orange ball works great and bounces just high enough that it doesn’t clear our patio wall, but enough for him to go and jump up to retrieve. But the glow in the dark ball is his favorite! Both me and my pomsky were blown away how we brought the glow in the dark ball outside just as the sun was going down, and when we brought it back inside it was BRIGHT AND GLOWY!!! Even with the sun going down and not much light left!! He loves to play with it in the house after it’s all glowy from the sun. Sometimes we even glow it up under a light inside the house if it’s dark outside, and he will play with it outside in the dark haha he loves it!!! And I think it’s pretty cool too!! All of the balls this company overs are VERY durable! We realized early on that we cannot give our dog regular tennis balls cuz his favorite thing to do is tear the outside fabric off!! So we were trying to figure out what company has the best balls for dogs so they can’t sit there and try to pry them apart, and we found this company!! We have already bought from them about 3 times and all of their balls are durable and bounce how they say they will bounce!! They have the super bouncers and the glow in the dark one and the one with angles that will bounce in different directions and the ones you can throw REALLYY far! Overall, all the balls from this company are very durable, bounce really good, and keep my dog occupied without allowing him to sit there and tear it apart!!! Which i love cuz I do not like when he stops playing with us to sit there and try to take off the fabric, cuz then he could swallow it! So always buy the balls from this company!!! They are the best and most durable!!!! Also plenty soft for your dogs mouth so they can chew on it a bit and it won’t hurt their teeth/gums!
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Reviewed in the United States on July 28, 2024

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