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Description
Mouse T-SOD ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Cell Supernatant: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse the tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Preparation before testing: 1. Please remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of gradient standard working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, and then gently mix (concentration is 10ng/mL). Then dilute to the following concentrations: 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.3125ng/mL, 0.15625ng/mL, and 0ng/mL. Serial dilution method: Take 7 EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this step to mix thoroughly. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Total Superoxide Dismutases (T-SOD) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP catalysis and to yellow by acid. The intensity of the color is positively correlated with the level of Total Superoxide Dismutases (T-SOD) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | ELISA Kit for Total Superoxide Dismutases | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Superoxide dismutase (SOD) is an enzyme that catalyzes the conversion of superoxide into oxygen and hydrogen peroxide through a dismutation reaction. It is widely found in various animals, plants, and microorganisms and serves as an important antioxidant, protecting cells exposed to oxygen. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell supernatant, tissue homogenate, etc. |
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4.7 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Xylimelts work...very very well..finally moisture to my mouth for longer than anything i have tried
Size: 80 ea
i have xerostomia...this is a dry mouth due to radiation treatments...I have minimum saliva. I must sip water or a liquid with every bite of food.
When i talk my mouth quickly dries and makes it difficult to speak......I have tried many....many different sprays, gels, etc. etc. Along came Xylimelts and it has been the best thing for me to date. It does add some moisture to my mouth and the great part is that it lasts for a very long time. I use the mint free only because my dry mouth is more sensitive to certain tastes. I once used Biotene and now i stay away from all of their products.....Biotene was around a while and marketed their product and had become very popular....Glaxo Smith Kline ( the big drug company that only cares about their profits over our health) purchased Biotene and had altered the product for the worse and they have done this with other products. They actually removed beneficial ingredients and replaced them with poor ingredients....Here is just one example: Xylitol was in one of their products ( Biotene toothpaste) when they purchased the company from Biotene. Xylitol has beneficial qualities and they replaced it with the Cancer in Laboratory rats ingredient called Sacharin... They have removed other ingredients as well...I stay away from any product from Glaxo Smith Kline. I have my own personal boycott and that means I no longer buy their Tums as well... Yes...They played their games with Tums also.....so i just do not buy their products.
Xylimelts does work for me...very very well. It adheres to the gums above my teeth and it just stays there adds moisture to my mouth. Obviously i recommend this product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 18, 2016
★★★★★ 4
I'd rate them 5 stars if only I'd had them to use over the last two decades!
Size: 80 ea
A couple of years ago my dentist and hygienist told me about Xylimelts, which I promptly ordered and appreciated as soon as I used them. After suffering teeth problems due to meds for years, it's too late for me to save my uppers -- dry mouth had decayed my teeth, even with plenty of hydration, good dental hygiene and wonderful dental care (when I didn't put it off a few times, sigh...), and I’m about to get dentures :( .
I started out putting two in my mouth at night, but that seemed to be a bit much -- they'd cause me to drool and stain my pillow (washed right out!) Mostly out of concern for the cost of Xylimelts, I began putting only one in my mouth at bedtime. This has worked well for the most part, though some nights I still wake up with a dry mouth, and sometimes put in another one. This could be due to allergies though, my nose is often stuffy as I sleep. However, two would probably prevent this from happening.
I tried the non-mint kind first, then accidentally ordered the mint kind once, but I prefer the non-mint flavor. It’s got no real taste, and that suits me fine. By the way, it does take 10 seconds to adhere them, but if you are not comfortable with that spot, you can move them to a better one without them losing their “stick” to the outside of your teeth or gums.
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Reviewed in the United States on August 24, 2017
★★★★★ 5
Dry mouth
Size: 80 ea
Finally something that works day or night. Easy to apply.
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Reviewed in the United States on April 4, 2026
★★★★★ 5
These are fantastic!!!
Size: 80 ea
All I can say is, "WOW." I've been fighting terrible dry mouth since going on cpap three months ago (my mask covers nose and mouth). My machine has a humidifier but that wasn't helping at all, even when turned up so high it was raining in my mask! The first nite I tried these lozenges it was like a gift from the angels! I used two, putting one on each side as per the instructions. It was a little weird sticking them to my molars at first; they didn't seem to want to stick but finally took hold. I also had to kind of adjust them so they didn't get knocked loose when I closed my jaws. But once they were in place they worked like a charm. I don't find the taste at all objectionable. It's kind of a very, very mild mint. I will *definitely* order these again. If you fight dry mouth, these are worth trying even though they're on the expensive side. They aren't as expensive as dental work if they end up protecting your teeth from cpap-induced cavities!
UPDATE - my oral hygienist told me that CPAP users often begin to develop cavities and other dental problems from using the device. She said that the use of xilitol, which is in these lozenges, night help stave off such problems. Here I am, after years of CPAP use, and I have had no dental problems other than a lost filling! These lozenges are a lifesaver!
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Reviewed in the United States on May 15, 2018
★★★★★ 3
It works well but uncomfortable
Size: 80 ea
I take blood pressure pills that dry my mouth out. The inside of my lips feel burned from being so try overnight. It's uncomfortable. I discovered these tablets and thought I'd give them a shot. I am struggling with them. I don't have a large mouth and these have to sit inbetween your gums and your lip or cheek. They are bigger than I would like. Plus they kind of hurt my gums. You're supposed to put them in place, wait ten seconds so they stick and then there is no further instruction other than they work for several hours. Do we just leave them in place all night because the uncomfortability makes it so I can't sleep easy. I also wear a nightguard on my top teeth to keep me from grinding. I love Xylitol, I bought some to use in my coffee in the day time so that part is a great thing. I just can't get past how uncomfortable it is to have them fixated in your mouth all night. One last thing... you're supposed to put two in. I did that the first night and it was like... NOPE. I let them sit for 5 minutes like it says and then I just dissolved the rest in my mouth because no way could I leave them in place and sleep. The next night I just used one on my bottom gums. It was still uncomfortable. If they could make them smaller, it would be way easier. Other than that, the Xylitol does work and my mouth isn't nearly as try when I wake up in the morning.
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Reviewed in the United States on June 22, 2025
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