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Description
Mouse PTGS2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 30 ng/mL). Then dilute to the following concentrations: 30 ng/mL, 15 ng/mL, 7.5 ng/mL, 3.75 ng/mL, 1.875 ng/mL, 0.9375 ng/mL, 0.46875 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 30ng/mL standard working solution into the first EP tube and mix thoroughly to make a 15ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a prostaglandin endoperoxide synthase 2 (PTGS2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of prostaglandin endoperoxide synthase 2 (PTGS2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Prostaglandin Endoperoxide Synthase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Prostaglandin Glycogenase Synthase 2 (PTGS2), also known as cyclooxygenase-2 or COX-2, is an enzyme encoded by the PTGS2 gene. It converts arachidonic acid (AA) into prostaglandins (PGs). It is a sequential homodimer. Each monomer of the enzyme has a peroxidase and a PTGS (COX) active site. PTGS (COX) catalyzes the conversion of arachidonic acid to prostaglandins in two steps. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.46-30ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.1 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Love the material, very comfortable
I got the black in a size small, and the fit turned out perfect. It’s relaxed enough to actually move in comfortably, and looks really nice for a plain athletic shirt. The fabric is super soft right out of the package, and the tagless construction and flat seams mean there’s nothing scratchy or irritating even after wearing it for hours.
The breathability and moisture wicking actually live up to its claims. It stays lightweight and cool in a way that cheaper workout tops just don’t, and the bamboo blend does seem to hold up surprisingly well on the odor resistance side of things too. The fabric is nice and stretchy while managing to not lose its shape as the day goes on, and so far the black has stayed vibrant through washing.
My only slight hesitation is the price. While not bad by any means, $20 for a single athletic t-shirt is a little more than I’d typically spend. Having worn it a few times though, I think the quality ended up justifying it.
Overall, if you’re looking for a workout top that actually delivers on its claims and holds up over time better than the cheaper versions, this one is worth the investment.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Great top for a heatwave
Color: Grey, Size: Large
We are in the midst of a heatwave in SoCal (95 degrees on St Patrick's Day, sheesh), and this tee really proved its claims today! I ordered it in gray, in anticipation of needing to deflect light, I just didn't think I'd need it this soon. At any rate, I love the coolmax plus bamboo plus vicose blend. I've learned to check the fabric of almost offering these days as somehow detestable synthetics (I won't say the P word, but it rhymes with Esther) because they trap heat, and the whole reason I'm buying summer clothing is to try to keep my body temperature on the cool side!
At any rate, this lovely blend did help keep me cool and dry, and the curved hem is really flattering. You might not think that such a small detail would make much difference, but I find myself reaching for this for that reason alone!
I ordered a Large, but likely shouldve gone with a Medium. It's a bit baggy but allows a lot of movement, so it's fine. I'm often between sizes and toss a coin pretty evenly split on which size I eventually settle on, so it literally is a real toss up. In the hope of helping someone else, I'm 5'5, about 145 lbs, 29 inch waist, and I wear a 34F/34DDD. I read another review that said that the shirt was too short, but I didn't find that to be true with mine. It fell to a flattering position, as in the photo, but maybe different colors have different sizing, as I've run into sometimes with major chains, and it's annoying. So take it with a grain of salt. I've already washed it and it doesn't shrink, nor did I expect it to.
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Reviewed in the United States on March 18, 2026
★★★★★ 4
Breathable and light
Color: White, Size: Small, Color: White, Size: Small
This shirt is truly better in person than in the stock photos. Its just a bit more of a textured athletic material in person that looks nicer on than a simple loose standard t shirt. The material does still sit semi loosely and only mildly hugs curves but its still figure complimenting and looks just a hair above only casual. Its very very breathable and lightweight, excellent for the gym or summer running etc but also serves as a phenomenal base layer for hiking and sports that carry into colder climates and seasons . Its well made and fots my size well so the size chart feels spot on. This shirt is really only losing points for me in the cost department because while its decent its nothing special. Its a good basic but its not something I couldnt find almost everywhere else.
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Reviewed in the United States on March 23, 2026
★★★★★ 5
Soft, slinky cooling tee without that synthetic feel
Color: Grey, Size: Medium, Color: Grey, Size: Medium
I ordered this bamboo viscose CoolMax t-shirt in gray, size medium because I wanted something lightweight and breathable for workouts and just being around the house when it is hot. The standout feature to me is the super soft and slinky feel it has without the overly cool wet feeling you get from other shirts made from synthetic “cooling fabric.” It feels smooth and comfortable against the skin while still doing a great job keeping me dry and not clingy. The fabric is lightweight with a nice stretch, and it moves easily without losing its shape.
The fit is very flattering with a clean shoulder line and a curved hem that sits right at the hips, so it looks polished enough to wear out, not just for the gym. The seams are flat and smooth, and the tagless design makes it easy to wear all day without irritation. It also holds up well after washing with no shrinking or shape issues. For sizing reference, I am 5'6", 119 pounds, 34B, with a 28.5" waist, and the medium fits me exactly like the model in the main listing picture, exactly. That should really help with sizing decisions if your build is similar. Highly Recommended!
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Reviewed in the United States on April 21, 2026
★★★★★ 5
Lightweight and comfortable to wear.
Color: White, Size: Medium, Color: White, Size: Medium
I wanted something comfy, lightweight, breathable, durable, and cool to wear while working out. My old t-shirts leave a lot to be desired. This Bamboo Viscose Coolmax T-shirt meets all the above. The only downside for me is that I chose white, and the material is very thin and see-through. If you prefer to dress more modestly as I do, you’ll want to select a darker color. I planned to only wear it in the house or while sitting outside on the patio, so it’s okay. Comfort and breathability are important to me when the summer heat and humidity hit.
The length and cut fit my physique and are comfortable to wear and move in. I got a medium, and it fits perfectly, which was really a nice surprise. Many tops tend to be too long or too tight in the hips. I’m 5’4” and 130 lbs.
The top washes up nicely, but ended up with a few wrinkles. No big deal for me, though.
Is it worth the price? I’d say it is based on the quality, fit, comfort, and breathability of the fabric. I’ve passed up buying similar tops when shopping because they were more expensive than this one. It’s a top to wear when working out, not for a night out on the town. It shouldn’t cost a fortune. At the time of this review, it’s on sale for $5.00 less, making it an even better bargain.
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Reviewed in the United States on April 3, 2026
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