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Description
Human PSEP ELISA KitProduct Specification Usage Experimental equipment required: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C constant temperature box 4. Distilled or deionized water Sample preparation and requirements: UrineOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre test preparation: 1. Remove the test kit from the refrigerator 10 minutes
Product Specification
| Usage |
Experimental equipment required: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C constant temperature box 4. Distilled or deionized water Sample preparation and requirements: UrineOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a prostatic exosomal protein (PSEP) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of prostatic exosomal protein (PSEP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human prostatic exosomal protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Prostatic exocytic protein (PSEP) is a small protein secreted by the prostate. PSEP effectively inhibits viral activity and also acts as an antibacterial agent, protecting sperm and enhancing sperm motility. When the prostate is inflamed, this protein is released into the urine, allowing for detection. It is an important tool for diagnosing chronic nonbacterial prostatitis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Urine and other biological fluids |
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A good product
Model: iPadAir 8/7/6th/iPadPro 6/5th 2026/25/24
This is easy to install and works well
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Reviewed in the United States on May 29, 2026
★★★★★ 4
Very Good iPad Screen Protector
Model: iPad 11th A16 2025/10th
I used this item to replace the protector screen that came with my iPad case. I found it to be very compatible using it in conjunction with the case. It was easy to install. It’s very clear. It looks great, and works well. The only problem I have is that it doesn’t resist fingerprints. On the other hand it’s super easy to clean.
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Ailun screen protector WORKS Great!
Model: iPad 11th A16 2025/10th, Model: iPad 11th A16 2025/10th
Lifesaver tempered glass cell phone protector. Smooth, thin and appears like the cell itself. Reactive to cell touch and no lag time.
I recently had a terrible accident from a heavy metal yeti mug fall onto my cell. I was livid when I realized my cell phone was sitting on the counter and was struck hard and thought my phone was severely damaged.
I remember I had placed the glass screen protector on and slowly peeled it off. To my happy amazed discovery, the Ailun screen protector truly protected my cell phone from damage. The stick on cover took the brunt of the metal mug falling 2-3 feet above my phone!!! Saved me alot of headache, money to replace the phone which was an important special gift, and anguish.
I will use this product again for future phones when I run out of cell life. Take a look of the cracked glass cover which helped me!!
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Great product
Model: iPad 11th A16 2025/10th
Great visibility, clear, cleans well, easy to install
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Strong almost scratch proof durable, no bubbles when installing
Model: iPad 11th A16 2025/10th
Stronger then it looks. Work construction and it's never let me down. Way easy to put on with no bubbles clear almost scratch proof. As work is hard on my phone perfect fit every time
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Reviewed in the United States on May 27, 2026