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Description
Mouse IL-24 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 24 (IL-24) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 24 (IL-24) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 24 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-24 (IL-24) is a protein in the interleukin family and a cytokine signaling molecule in the immune system. IL-24 is encoded by the IL24 gene. IL-24 belongs to the IL-10 cytokine family and signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as melanoma differentiation-associated 7 (MDA-7) because it was discovered to be a tumor suppressor protein. IL-24 controls cell survival and proliferation by inducing rapid activation of specific transcription factors, STAT1 and STAT3. This cytokine is primarily released by activated monocytes, macrophages, and T helper 2 (Th2) cells and acts on the skin, lungs, and reproductive tissues. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Great quality Soap
Scent: Oatmeal Shea (Unsented)
This natural bar soap is absolutely fantastic! I’ve purchased it multiple times now, and it has never disappointed. The quality is consistent with every bar — it lathers well, cleans thoroughly, and leaves my skin feeling fresh without any dryness.
I really appreciate that it’s made with natural ingredients, and the scent is clean and refreshing without being overpowering. It lasts a good amount of time, making it a great value as well.
Overall, this is a high-quality soap I keep coming back to. The fact that I’ve bought it multiple times says it all — it’s reliable, gentle, and one of the best natural soaps I’ve used. Highly recommended!
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Reviewed in the United States on November 7, 2025
★★★★★ 3
Sometimes Size Does Matter
Scent: Alpine & Spice, Scent: Alpine & Spice
Let me just get this out of the way now the soap itself smells decent, nothing wrong there. I only wish I got another cent. Not that this one smells bad it’s just it wasn’t my cup of tea. I’m mostly disappointed about the size because I’ve been through this before. The soaps themselves are not big enough to fill out the box as you can spot in some of the pictures and video. I honestly feel like the soap size was different in the pictures of the customer reviews that I saw. Each bar may last me a week and a half and that’s with a soap saver. To be honest, the box that it came in would fit two decent size soaps if they filled out the box.
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Reviewed in the United States on November 19, 2025
★★★★★ 5
As expected
Scent: vanilla orange
Love the scents of this soap. It's gentle on my skin and leaves me clean, fresh and soft.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
A Comprehensive Balanced History of the Guadalcanal Campaign -- Must Read!
Format: Hardcover
I've read a number of good books on the Guadalcanal campaign, and always thought that "Neptune's Inferno" by Hornfischer was the absolute best.
I was wrong. Although Hornfischer does a superb account of the Navy and its travails and triumphs, Inferno doesn't delve deeply into the Marine (and Army) land battles. This book does both. Moreover, it provides a continuous timeline of both, and does so in such a way that the reader better understands both as related actions. For instance, I was never really aware that for the first three and a half months the Americans controlled the seas during daylight, and the Japanese at night (sounds a little like Viet Nam). The November 13 sea battle between Americans and Japanese -- in which US cruisers took on Japanese battleships and two American admirals died -- was in fact a clash of a major last ditch effort by the Japanese to reinforce their troops and destroy Henderson Field, which would have allowed them to control the seas both day and night. By that time there had been multiple bloody battles ashore between the Marines and Japanese, with the balance favoring the Marines, but if the Japanese had wrested control of the airfield and seas that would have been old history. The book includes a good view from the Japanese perspective, and some little known historical tidbets as well, e.g., Guadalcanal received its name from the Spanish home town of a ships officer who accompanied Spanish explorer Don Alvaro Medana, who discovered the island during a 1568 expedition to discover the fabled King Solomon's gold mines. Who knew? :-)
Bottom line: I highly recommend this book, both for its balanced coverage of the entire campaign, land and sea, and even more for its integrated narrative -- you know what was taking place (or had taken place) on almost a day-by-day basis, which allows the reader to fully appreciate how actions ashore influenced those at sea, and vice versa.
IMO, a must read, even for those who thought (like me) that they knew it all!
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Reviewed in the United States on October 12, 2017
★★★★★ 5
The Island Of Death
Format: Hardcover
On August 7th, 1942, American Marines stormed ashore on Guadalcanal. What lay before them was a six-month odyssey of fighting against the best of what the Japanese had to offer. In this fine book, author Joseph Wheelan describes the battle that turned the tide in the Pacific War.
The Japanese had started construction of an airfield on Guadalcanal. If completed, Japanese aircraft would be able to harass American convoys and threaten Australia. The Americans seized the airfield and eventually, planes from the Cactus Air Force began attacking the Japanese.
Throughout the book, the reader learns about all phases of the Guadalcanal campaign, including the battles of Alligator Creek and Bloody Ridge. On the sea, the Americans and Japanese slugged it out at Savo Island, as well as the great naval battles of November, 1942. Names such as Chesty Puller, John Baslone, "Archie" Vandegrift, Joe Foss, and "Bull" Halsey became household names in the United States.
Each side lost many men, ships, and planes, but the tenacity and, finally, the industrial might and the ability to rapidly replace losses, led the Americans to victory. Never again would the Japanese regain the offensive in the Pacific War.
"Midnight in the Pacific" is a very good book, and the author has done a good job of describing all of the main points of the battle. Each chapter is broken down into a single month's worth of action, and the narrative is well-written. Highly recommended.
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Reviewed in the United States on September 25, 2017