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Description
Human BASP1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue, homogenates, cell lysates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue, homogenates, cell lysates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Brain Abundant, Membrane Attached Signal Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Brain-enriched membrane attachment signaling protein 1, also known as BASP1, is encoded by the BASP1 gene. This gene encodes a membrane-bound protein with several transient phosphorylation sites and a PEST motif. The conservation of proteins with PEST sequences across species supports their functional significance. PEST sequences are often found in proteins with high turnover rates. The immunological characteristics of this protein are species-specific. This protein also undergoes N-terminal myristoylation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissuehomogenates, celllysates and otherbiologicalfluids |
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★★★★★ 5
lightweight, effective, and easy to try
I started using this on under eye dark spots after a friend recommended it. The texture is lightweight, non sticky, and absorbs smoothly. The price is very reasonable, making it great for trying out. After about four days, my dark spots looked slightly lighter, and I plan to continue using it for more noticeable results.
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Reviewed in the United States on March 31, 2026
★★★★★ 5
Satisfied…..
I never thought I’d be interested in products like this, but as I’ve gotten older, I realized I need something with stronger effects than what I used before. I happened to come across this product while searching, and after trying it, I feel it’s quite good for the price. Of course, skin that has been damaged over many years can’t be fully restored by just one product, but I’m satisfied with even the slight improvements I’ve noticed.
I think I will prefer again for sure.
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Love it!
Gentle yet effective. My skin texture has improved significantly since I started using this Celdyoué serum. Perfect for my nightly routine.
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Reviewed in the United States on March 30, 2026
★★★★★ 5
So lightweight, so smooth
I was looking for a retinol eye cream and decided to try this since I liked this brand before.
The texture is super lightweight and absorbs well, and my makeup doesn’t pill at all after using it.
Not sure yet about long-term wrinkle results, but it keeps my under-eye really hydrated and smooth. I’ve also been using it on my smile lines. lowkey excited to see more results!
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Reviewed in the United States on March 31, 2026
★★★★★ 3
Helped a bit with barrier, but it must be used very sparingly, and didn't improve fine lines for me.
I had mixed feelings about this eye cream. On paper, the combination of ingredients such as retinol, niacinamide, and centella sounded promising, especially for targeting texture and supporting the skin barrier. However, in practice, I found the formula to be a bit too strong for my sensitive eye area. I had to be very careful not to apply it too close to my eyes to avoid irritation. The cream is very thin and lightweight, and I found that it did absorb well without leaving too much of a greasy residue once applied.
After about two weeks of consistent use, I did notice some positive changes. The skin under my eyes felt slightly more elastic, and my skin barrier seemed to be benefiting overall, which I appreciated. That said, I didn’t see any real improvement in fine lines or firmness, which is one of the main reasons I reach for an eye cream with retinol in the first place.
The price point is definitely affordable, but it also feels like a case of getting what you pay for. While it’s not a bad product, it didn’t stand out enough for me to consider replacing my regular eye cream.
At the end of the day, skincare is a long game. What we do now often shows up years down the line, so I do think it’s worth investing in higher-quality formulas when possible. For me, this one was just okay: some mild benefits, but ultimately unremarkable.
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Reviewed in the United States on May 3, 2026