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Description
Mouse anti-CCPAb ELISA KitProduct Specification protein anti CCPAb Usage Specimen requirements 1. Specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in 20 Store, but avoid repeated freezing and thawing 2. Unable to detect containing NaN3
Product Specification
| protein | anti-CCPAb | ||||||||||||||||||||||||||||||||||||
| Usage |
Specimen requirements
Operation steps
2. Sample addition: Blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested are set respectively. Accurate spike of standard on enzyme-labeled coated plate 50μl, first add sample diluent to the sample well to be tested 40μl, then add the sample to be tested 10μl (The final dilution of sample was 5 Times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well.
calculate
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| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||||
| Theory | This kit uses double antigen sandwich method to determine the level of mouse anti-cyclic citrullinated peptide antibody (anti-CCPAb) in specimens. Coating microplates with purified antigen to prepare solid phase antigen, adding anti-cyclic citrullinated peptide antibody (anti-CCPAb) to the coated microwells sequentially, and then combining with HRP-labeled antigen to form antigen-antibody-enzyme-labeled antigen complex, which is thoroughly washed and then added with substrate TMB to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and the anti-cyclic citrullinated peptide antibody (anti-CCPAb) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of mouse anti-cyclic citrullinated peptide antibody (anti-CCPAb) in the sample was calculated by a standard curve. | ||||||||||||||||||||||||||||||||||||
| Source | Mouse | ||||||||||||||||||||||||||||||||||||
| Synonym | Mouse anti-cyclic citrullinated peptide antibody (anti-CCPAb) ELISA kit | ||||||||||||||||||||||||||||||||||||
| Detection Type | It is used to determine the content of anti-cyclic citrullinated peptide antibody (anti-CCPAb) in mouse serum, plasma and related fluid samples. | ||||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||||||||||||||
| Test Range | 3ng/L - 90ng/L |
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