SKU: 75486455298

Streptococcus pyogenes SpyCatcher001, His tag

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Description

Streptococcus pyogenes SpyCatcher001, His tagProduct Specification Species Streptococcus pyogenes Expression System E. coli Molecular Weight Predicted MW: 14. 3 kDa Observed MW: 17 kDa Purity 90% by SDS PAGE Endotoxin <1EU g Physical Appearance Lyophilized Powder Storage Buffer Lyophilized from a 0. 2 m filtered solution of 0. 2M PBS, pH7. 4. Reconstitution Reconstitute no more than 1 mg mL according to the size in deionized water after rapid centrifugation. Stability & Storage 12 months from

Product Specification


Species Streptococcus pyogenes
Expression System E.coli
Molecular Weight Predicted MW: 14.3 kDa Observed MW: 17 kDa
Purity >90% by SDS-PAGE
Endotoxin <1EU/μg
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

The SpyTag/SpyCatcher system is a technology for irreversible conjugation of recombinant proteins. The peptide SpyTag (13 amino acids) spontaneously reacts with the protein SpyCatcher (12.3 kDa) to form an intermolecular isopeptide bond between the pair. DNA sequence encoding either SpyTag or SpyCatcher can be recombinantly introduced into the DNA sequence encoding a protein of interest, forming a fusion protein. These fusion proteins can be covalently linked when mixed in a reaction through the SpyTag/SpyCatcher system. Using the Tag/Catcher pair, bioconjugation can be achieved between two recombinant proteins that would otherwise be restrictive or impossible with traditional direct genetic fusion between the two proteins. For example, issues regarding protein folding, suboptimal expression host, and specialized post-translational modifications can be alleviated by separating the production of the proteins with the modularity of the Tag/Catcher system.

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Exchange/Return Notes
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SKU: 75486455298

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4.7 ★★★★★
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omri
Lake Worth, US
★★★★★ 5
good shoes
Size: 8 Toddler, Color: Black, Size: 8 Toddler, Color: Black
fit great the child like it
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
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Verified Purchase
Carly Perez
Fort Morgan, US
★★★★★ 5
Toddler approved
Size: 8 Toddler, Color: Black
My kid loves these shoes. We’ve bought them multiple times. She can get them on and off easily and they stand up to her wild adventures.
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Reviewed in the United States on May 3, 2026
A
Verified Purchase
Allyssa
Lake Worth, US
★★★★★ 5
So cute and so worth it!
Size: 3 Little Kid, Color: Turquoise
AMAZING for my 7 year old daughter. She can tie shoes but I no longer have to wait on her to do so. It takes 2 seconds for her to put these on and she says that they are super comfortable. We have been to Silver dollar City multiple times and she never complains about her feet hurting! They are easily washed in the washer. They hold up great. I think they are very much worth the money! Fit normal.
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Reviewed in the United States on December 8, 2025
M
Verified Purchase
Morgan
West Palm Beach, US
★★★★★ 4
Our everyday shoe!
Size: 13 Little Kid, Color: Turquoise
Great shoes and good fit. Fits as expected. The sole has worn down a bit quicker than I had hoped but my kiddo wears them everyday. I would buy them again for sure!
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Reviewed in the United States on November 30, 2025
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Verified Purchase
lisa shelko
Natrona Heights, US
★★★★★ 5
So comfy !
Size: 2 Little Kid, Color: Lavender
Shoes are so comfy and stable . I love em !
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Reviewed in the United States on May 20, 2026

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