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Description
Rat F5 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Coagulation Factor V (F5) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Coagulation Factor V (F5) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Coagulation Factor V ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Coagulation factor V (FV) is a protein of the coagulation system, rarely referred to as a pro-accelerator or vulnerability factor. Unlike most other coagulation factors, it lacks enzymatic activity and instead functions as a cofactor. Defects can lead to a bleeding tendency, while some mutations (most notably factor V Leiden) predispose to thrombosis. The factor V gene is located on chromosome 1 (1q24). It is genomically associated with the multicopper oxidase family and is homologous to factor VIII. The gene spans 70 kb and consists of 25 exons, producing a protein with a relative molecular mass of approximately 330 kDa. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.1 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Tastes great!
Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12)
Multiple times re-ordering, this is a great tasting drink. That is quick and easy to grab for everybody in the house. Full of protein taste great and healthy. Makes me feel good. Having even the kids grab one on their way out the door if they are missing a meal or need a quick snack or pick me up before sports we currently need to re-order so I don’t have any pictures to post but if you’re thinking about it I would get them .
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Probably the best
Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12)
I'm not a super meat head muscle workout guy, but I went from being very active about my nutrition and macros and then had an accident which left me disabled so my needs changed. This fits different needs, but is still really really exceptional.
If you're a gym rat just looking for *only* plain old bulk protein to take after workouts, or you reeeeeeallly take a lot of it, honestly I'd get a big giant tub of bulk powder from like muscle milk or optimum nutrition. The price is just too good to ignore, & plus you can add another scoop or two to double or triple the protein. You can't really beat it, other than taste.
Speaking of taste, whether it's a pre-mix or powders, I've never, ever ever ever tasted a protein shake that tasted as good as this chocolate one. Dozens & dozens of brands over the decades, & the only one that comes close was the OG Muscle Milk recipe in the old tetra packs from like 15-20 years ago, before they were bought by Gatorade or whoever. This has NO right tasting as good as it does, with as little sugar, calories, & fat. PLUS having artificial sweeteners like stevia & sucralose. One reason I despise pre-made shakes, zero calorie & low sugar drinks is because I have a very strong detection of artificial sweeteners, but I can barely tell these are there. On top of that, 42 grams of protein in 14floz? I don't know what magic Fairlife did, but it's incredible.
One thing I will say though, is if you're taking a protein drink for pretty much anything other than supplemental protein replacement, this doesn't offer too much else. The "vitamins & minerals" aren't too impressive, and though a high protein drink can make you feel full or satiated, calories & fat are important too. As stereotyped it is, something like Ensure has a really good blend of calories, fat, sugar, and a lot of vitamins. There is a reason it's used all over hospitals & nursing homes. Plus the Ensure "Plus" & "Complete" have 16g & 30g protein each, respectively. They don't taste as good as the Fairlife does, but they're actually not too bad, since they use actual sugar. However, those really are meant for convalescing & true meal replacement, not bulking & training.
The only other downside is price really, at $40-45 for a 12 pack, these aren't cheap. However, when compared to a good alternative like optimum gold at about $25 for a 12 pack, you're getting almost double the protein in each drink from 24 to 42, and they're 14oz instead of 11oz, so you're getting 168oz compared to 132oz, which is like getting 3 more drinks worth. It's still a bit costly per bottle, as an Optimum Nutrition/Pure Protein/Premier Protein 11oz pre-made at $25 a 12pk will be a little over $2 each, & the Fairlife 12pk @ $45 is about $3.75 each. Still cheaper than if you went to pick one up at the gym or a gas station or something, a single would probably be $5-7, so there's value there in bulk. If it's worth it to you, that's up to your wallet.
Another plus is they are SNAP/EBT eligible, so that's a massive W.
If you take loads & loads & loads of protein every single day, and lugging a shaker with powder around isn't a problem, bulk powder is gonna be a LOT cheaper. However, if you only drink one every couple days, and don't want to deal with the blender/shaker hassle, and most importantly want the best tasting shake, this is it. This stuff is awesome, & I'll 1000% be trying the vanilla and strawberry as well. The chocolate is an easy 5 and worth trying.
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Reviewed in the United States on April 13, 2025
★★★★★ 5
Yummy taste!
Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12), Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12)
I’ve been using the Core Power Elite protein shakes and they’ve honestly become a go-to. The taste is super smooth and not chalky at all—more like chocolate milk than a typical protein drink. Each bottle packs 42g of protein, which is perfect after a workout or when I need something quick and filling.
I also love that it’s lactose-free and easy on my stomach. Great for busy days, gym recovery, or hitting protein goals without overthinking it. Definitely keeping these stocked
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Reviewed in the United States on April 24, 2026
★★★★★ 5
The Best Protein Drink I've Ever Had!
Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12)
These are hands down my favorite protein shakes. I’ve tried quite a few different brands over the years, but I honestly don’t drink any other protein drinks now besides these. The chocolate flavor is absolutely delicious and tastes much better than most protein shakes I’ve had.
They’re smooth, not chalky whatsoever like other protein drinks, and have a great balance of sweetness without tasting artificial. The high protein content is also a huge plus for post-workout recovery or a quick meal on busy days. I always keep these stocked in my fridge and restock every other week!
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Reviewed in the United States on May 28, 2026
★★★★★ 5
No chalky aftertaste
Flavor Name: Chocolate, Size: 14 Fl Oz (Pack of 12)
Been buying this product for years. Good amount of protein for pre/post workout. A little pricey but worth it.
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Reviewed in the United States on May 9, 2026
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