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Description
Mouse IL27 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 500pg/mL). Then dilute to the following concentrations: 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.8125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 500pg/mL standard working solution into the first EP tube and mix thoroughly to make a 250pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 27 (IL27) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 27 (IL27) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 27 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-27 (IL-27) is a heterodimer composed of EBI3 and p28 subunits linked by disulfide bonds. IL-27 promotes Th1 and Tr1 differentiation and inhibits Th2, Th17, and Treg responses, playing a crucial role in immune regulation. IL-27, a member of the IL-12 family, is primarily produced by antigen-presenting cells, including dendritic cells and macrophages, in response to microbes or other immune stimuli. Its receptors are widely distributed on various immune cells. IL-27 signals are transmitted intracellularly through the heterodimeric receptor IL-27R, which is located on cells. IL-27R is composed of WSX-1 and gp130 subunits. WSX-1 and gp130 are expressed on a variety of cells, including T cells, NK cells, B cells, and myeloid cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.81-500 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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★★★★★ 5
Off Campus Book Series
Format: Kindle
I absolutely love the Off Campus TV Series!! I have watched it 5 times already. I ordered the Off Campus Book Series. I just finished reading Book 1 - The Deal. It was absolutely amazing!! It was well written and I fell in love with Garret and Hannah. Love the Characters and both their storylines. I was so excited when reading in the Book that the Hockey Championship was being held at the Wells Fargo Center, Home of the Philadelphia Flyers, in Philadelphia, Pennsylvania. I live 30 minutes from (Philly) Philadelphia Pennsylvania. I would like to shout out to the Author ✍️ very well written!! Can’t wait to read the whole Book 📕 Series!!
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Reviewed in the United States on May 30, 2026
★★★★★ 5
The Deal
Format: Kindle
Loved it! Funny, sweet, passionate, hockey, friends, sexy all rolled into it! Loved Welles and Graham. Their banter was so fun! Can’t wait to get to book 2!!
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Love this cleanser
Size: 13.52 Fl Oz (Pack of 1), Style: 13.52 Fl Oz (Bottle)
I really love their products. This one in particular stands out to me bc I get oily around my chest and get little superficial pimples on my arms/neck and back and WOW!! This stuff really gets them gone! I use this all over my body the oily parts and I just love it! No smell at all. Very gentle. Not greasy! Great deal for the size and it’s been a game changer. I’ve realized I’m sensitive to even sensitive skin cleansers and this one doesn’t irritate at all!
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Reviewed in the United States on May 21, 2026
★★★★★ 5
La Roche-Posay Toleriane Cleanser: Gentle Care for Sensitive Skin 💧✨
Size: 13.52 Fl Oz (Pack of 1), Style: 13.52 Fl Oz (Bottle)
The La Roche-Posay Toleriane Cleanser is a fantastic skincare product for anyone with sensitive or easily irritated skin. Its gentle formula effectively removes dirt, oil, and makeup without leaving the skin feeling dry or tight. The lightweight texture feels refreshing, and it helps maintain the skin’s natural moisture barrier. The large bottle with pump packaging is convenient for daily use and lasts a long time. Overall, it’s highly recommended for those looking for a reliable, dermatologist-inspired cleanser that leaves skin feeling clean, soft, and balanced.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Finally a Cleanser That Doesn’t Trigger My Breakouts
Size: 13.52 Fl Oz (Pack of 1), Style: 13.52 Fl Oz (Bottle)
I've been using this facewash twice a day for about a month now and out of all the cleansers I've tried this is definitely one of the few that have not made my acne worse. Usually my skin reacts within a week or two of trying new products but with this product it remained calm while still providing assistance for excess oil and little breakouts on my face.
After cleansing with this product, it leaves my skin feeling clean but without that "overdried" feeling which is something that very rarely happens for me. The texture of the product is a thin gel that foams easily after using only a small amount, and it will rinse off completely and leave no residue behind when you're done using it. The product has no noticeable scent and feels extremely gentle even on days when my skin is more irritated or sensitive.
The price of this product is slightly higher than some of the other basic drugstore cleansers, but the amount of time I have been able to use this product has been remarkable. If you're someone who wears heavy makeup on a daily basis, you may think that this product is not strong enough or you may feel like it's too gentle; however, it has proven to be a reliable and low-maintenance product for anyone with acne prone/sensitive skin types.
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Reviewed in the United States on April 3, 2026
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