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Description
Mouse AdipoR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with adiponectin receptor 2 (AdipoR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Adiponectin receptor 2 (AdipoR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Adiponectin Receptor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Adiponectin receptor 2 (AdipoR2) is a protein encoded by the ADIPOR2 gene. It is a member of the progesterone and adipoQ receptor (PAQR) family and is also known as PAQR2. Similar to G protein-coupled receptors (GPCRs), AdipoR2 possesses seven transmembrane domains. However, AdipoR2 is oriented on the membrane in the opposite direction to GPCRs (i.e., cytoplasmic N-terminus, extracellular C-terminus), and does not bind to G proteins. AdipoR1 and AdipoR2 act as receptors for globular and full-length adipoins, mediating increased activity of AMPK and PPAR-α ligands, as well as fatty acid oxidation and glucose uptake by adipoins. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
Has worked for me
Number of Items: 1, Style: X10 Pro Omni Accessories Kit
All the parts fit my Eufy vacuum/mop perfectly
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 29, 2025
★★★★★ 5
perfect fit for the L40 Ultra
Color: L40 Ultra Accessories
I'm very pleased with this accessories kit. All the included items work perfectly with the L40, and are good quality, fit correctly, and cause zero issues. They install just as the OEM items do, and the L40 works exactly the way it did when it came out of the box. I was particularly pleased that even though it's a replacement, it came with another little screwdriver, as I'd misplaced the original.
These hepa filters fit snugly, stay in place, and work to take up dust and trap it rather than just spread it around the house. The mop pads appear to be good quality and get the job done. I like how they attach - easy to put on and take off, just like the OEM, so I can clean them easily and use them for a little longer. The main brush is so close to identical that I can't really tell them apart, and they work as advertised - little wrapping and tangling, and they deal with dog and cat hair without a hitch.
In all, this is a good value for replacement components that work and are good quality. Recommended.
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Reviewed in the United States on May 2, 2026
★★★★★ 5
These Mop Pads Made My Hardwood Floors Shine Again!
Color: L40 Ultra Mop Pad, Color: L40 Ultra Mop Pad
I picked up this 8‑pack of mop pads for my Dreame X40 Ultra, and they’re exactly what I needed. These attach with Velcro, so swapping them out is as simple as pulling off the old pad and pressing on the new one. No tools, no fuss.
My original pads lasted a long time, but nothing beats the feeling of putting on fresh, fluffy mop pads and watching the robot make the floors shine again. These are the same size, same fit, same performance as the originals.
🧹 Compatibility
Dreame and MOVA are basically sister companies, so these pads don’t just fit the X40 Ultra — they also work with a ton of other models, including:
- L40 Ultra
- X40 Ultra
- X50 Ultra
- L50 Ultra
- L10S Ultra
- L10S Pro Ultra Heat
- L10S Ultra Gen2
- L40S Ultra
- X30 Ultra
- L10 Ultra
- And yes — MOVA models like the E40 Ultra
If you’ve got a Dreame or MOVA robot, chances are these pads will fit perfectly.
🪵 Cleaning Performance
These pads leave my hardwood floors looking clean and streak‑free. The robot spins them with enough pressure to lift dirt, dust, and whatever the dogs track in. Fresh pads always make a difference, and this pack gives you plenty of backups.
💰 Value
Eight pads at this price is a steal. OEM pads cost way more, and these perform just as well. Great fit, great quality, great results.
⭐ Final Thoughts
Easy to install, perfect compatibility, and they make my floors look amazing. Couldn’t be happier with the purchase. I’ll definitely keep a pack of these on hand.
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Reviewed in the United States on March 29, 2026
★★★★★ 5
Exceptional Value – Identical Quality to OEM Parts!
Color: L40 Ultra Accessories, Color: L40 Ultra Accessories
I recently received this replacement accessories kit for my robot vacuum, and I couldn't be more impressed. To be honest, I was a bit skeptical at first—usually, when you buy third-party bundles, you expect some trade-off in material quality or fit, but that is absolutely not the case here.
Packaging & Delivery
The kit arrived exactly as described and was packaged very securely. Everything was organized neatly inside the box, ensuring that the side brushes weren't bent and the HEPA filters remained pristine during transit. It’s clear the manufacturer took care to make sure the product arrives in perfect condition.
Quality & Performance
After comparing these parts side-by-side with the original factory components, I genuinely cannot see any difference in quality.
• The Main Brushes: The rubber material feels durable and has the same flexibility as the original, picking up hair and debris without any issues.
• The Mop Pads: These are thick, absorbent, and the Velcro backing aligns perfectly with the vacuum’s mop plates.
• Filters & Bags: The HEPA filters fit snugly with a proper seal, which is crucial for maintaining suction power, and the dust bags are made of a thick, multi-layer material that feels very reliable.
Final Thoughts
This set is a fantastic value. Buying these components individually or from the name brand would easily cost double or triple the price. You get a massive supply of consumables that will likely last me over a year. If you are looking for a way to maintain your vacuum’s peak performance without overspending on "official" replacements, this is the kit to get. Everything snaps into place effortlessly and works perfectly.
Highly recommended!
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Reviewed in the United States on March 19, 2026
★★★★★ 4
Great Vacuum Kit
Color: L40 Ultra Accessories, Color: L40 Ultra Accessories
I'm not sure if I didn't read carefully enough, or if the title/description changed afterward, but I got this for our Dreame X50, which is similar to the X40 but not quite the same. It looks like everything in this kit will work with ours, except the rollers. The rest of it, the bags, the filters, and the brushes, look the same though, and I can confirm that the mop pads work. Overall, it looks like a great set, full of everything you'd need to freshen up the robot that keeps your house fresh.
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Reviewed in the United States on April 3, 2026