SKU: 70540141626

Human sFlt-1, His tag

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Description

Human sFlt-1, His tagProduct Specification Species Human Synonyms Soluble fms like tyrosine kinase 1 Accession P17948 2 Amino Acid Sequence Protein sequence(P17948 2, Ser27 His687, with C 10*His)

Product Specification


Species Human
Synonyms Soluble fms-like tyrosine kinase 1
Accession P17948-2
Amino Acid Sequence

Protein sequence(P17948-2, Ser27-His687, with C-10*His)
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEITIRGEHCNKKAVFSRISKFKSTRNDCTTQSNVKHGGGGSHHHHHHHHHH

 
Expression System HEK293
Molecular Weight Theoretical: 76.4kDa Actual: 100kDa
Purity

＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.  
  • Please avoid repeated freeze-thaw cycles. 

Background

Soluble fms-like tyrosine kinase-1 (sFlt-1 or sVEGFR-1) is a tyrosine kinase protein with antiangiogenic properties. A non-membrane associated splice variant of VEGF receptor 1 (Flt-1), sFlt-1 binds the angiogenic factors VEGF (vascular endothelial growth factor) and PlGF (placental growth factor), reducing blood vessel growth through reduction of free VEGF and PlGF concentrations. In humans, sFlt-1 is important in the regulation of blood vessel formation in diverse tissues, including the kidneys, cornea, and uterus. Abnormally high levels of sFlt-1 have been implicated in the pathogenesis of preeclampsia.

 

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 70540141626

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4.3 ★★★★★
Based on 21 reviews
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Product Reviews
R
Verified Purchase
Rhbones
Houston, US
★★★★★ 5
Works for my 2011 115hp Etec
Size: For 75-250 HP
Exact fit and cheaper than a trip to a repair shop. Make sure you have the proper gasket sealer, a good scrapper that's not going to dig into the sealing surface, and tools for the job. I went on my computer and downloaded a video that walked me through the process. I'm a mechanic by trade but wanted to ensure everything was correct. I've been running the motor now for a couple of months. Peeing just as it's supposed to. No more delivering, waiting, and retrieving my boat from the shop for its routine service. Just follow your owner's manual to make sure you do all the necessary maintenance.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 8, 2025
C
Verified Purchase
Chelsey R
San Leandro, US
★★★★★ 5
Great replacement
Size: For 5.5HP 6HP 7.5HP
Works as it should. Easy to swap out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 12, 2025
D
Verified Purchase
Dan
Chelsea, US
★★★★★ 5
Works
Size: For 60 65 70 75 HP
Works great
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
B
Verified Purchase
Billy kinney
Phoenix, US
★★★★★ 4
Great product
Size: For 85-300 HP
Complete rebuild kit for $38 and great quality and fast delivery
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 30, 2025
G
Verified Purchase
grog
Cuba, US
★★★★★ 5
If pump doesn't seem to work
Size: For 85-300 HP, Size: For 85-300 HP
Hi....I apologize it took me a while to get back to you with my results. Here is what I discovered about the pump and will also apply to some of the other comments I read in the reviews with people not getting the pump to work: 1.) I removed the pump to check the proper direction of the impeller and it was correct as can be seen in the photo. 2.) I built a pump shaft attachement so that I could operate the pump with a power drill. 3.) I attached the water muff to the unit and tried to pump water.....it wouldn't do so. What I did see however was that even at high hose discharge and small leakage at the muff I could not fill the water cavity in the housing. 4.) I installed a strap around the muff and tightened it very tight and THEN at high hose pressure I was able to get the cavity to fill with water. 5.) At that point I activated the drill and was able to get the pump to actually pump water. I then removed the pump housing and removed the O-Ring that resides on top of the impeller, reassembled and it would not pump water. Since the O-Ring sits higher than the original does I wanted to make sure that it was necessary to have it in the pump. 6.) I reassembled the engine and kept the tight strap on the bottom and finally it pumped water into the engine. My conclusion that you can reply to other buyers if they have a problem: Do NOT underestimate 2 things....1.)the lack of ability of the pump to draw water up AND 2.)the fact that if the muff leaks it will NOT fill the cavity when common sense says it should. This was a 120hp Johnson. The engine bottom must be immersed fully or the muff must be very tight on the lower unit for the pump to function. Please feel free to post this if you wish.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 12, 2024

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