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Description
Human Rap2c ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against RAP2C, Member of the RAS Oncogene Family (Rap2c). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of RAP2C, Member of the RAS Oncogene Family (Rap2c) in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human RAP2C, Member Of RAS Oncogene Family ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Ras-associated protein Rap-2c (Rap2c) is a protein-coding gene. It is a novel member of the Ras family, belonging to the Rap branch of small GTPase proteins, and may be involved in SRE-mediated gene transcription. Pathways associated with this gene include ERK signaling and the innate immune system. Gene Ontology (GO) annotations associated with this gene include GTP binding and transcriptional coactivator activity. An important homologue of this gene is RAP2A. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Coop Home Goods Eden Adjustable Memory Foam Pillow with Cooling Gel, Bam
Team Name: Eden, Size: Queen, Color: Classic, Team Name: Eden, Size: Queen, Color: Classic
Experience customizable comfort and proper neck support
The Coop Home Goods Eden pillow is designed with shredded memory foam infused with cooling gel to provide balanced support and pressure relief for your head and neck.
Cooling gel memory foam for a fresher sleep
The gel-infused foam promotes airflow and heat dissipation, helping you stay cool throughout the night.
Fully adjustable loft and firmness
Add or remove filling to customize the height and firmness to match your sleeping position, whether you sleep on your side, back, or stomach.
Premium bamboo-derived cover
The soft, breathable cover made from bamboo-derived viscose and polyester is removable and machine washable for easy cleaning.
Hypoallergenic and certified safe materials
Made with CertiPUR-US and GREENGUARD Gold certified foam, resistant to dust mites and harmful chemicals.
Ideal for neck pain relief and better sleep quality
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Reviewed in the United States on January 31, 2026
★★★★★ 5
Quality & Comfort with Fully Adjustable Support
Team Name: Eden, Size: Queen, Color: Classic
I used to buy pricey, handmade, 100% down pillows—until I learned about the cruelty behind down production. I stopped using down entirely: pillows, comforters, jackets, everything. Then began the long search for something that could at least come close to the comfort of down, I tried Tempur and countless others, but nothing came close.
As a side sleeper, I need a pillow that’s high, SOFT, supportive, conforming, and huggable. After wasting money on at least five different pillows, I finally tried the Coop Home Goods Eden Pillow because of its adjustability. The cover is kind of thick, soft, and well‑made (I use a nice cotton pillow cover over it). The filling is soft and high quality. You can customize the height and firmness however you like. It even comes with plenty of extra fill.
To my surprise, the shredded foam doesn’t make the pillow clumpy—something every other similar pillow I tried struggled with. The surface stays smooth and soft, and it’s been a total game‑changer. It’s a keeper! I’ve had mine for almost four years now and only needed to add more filling once, about 8–10 months ago. Toss it in the dryer on low with a couple of tennis balls, and it fluffs up like new. And the icing on the cake: outstanding customer service.
I highly recommend this pillow and will definitely buy it again
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Reviewed in the United States on December 26, 2025
★★★★★ 3
Nothing like the original Eden, don’t buy the name
Team Name: Eden Cool+, Size: King, Color: Classic
I’ve had the original Coop Eden for 5+ years and it has been a great pillow, very comfortable, soft but supportive but when I purchased a new mattress I wanted to refresh my pillow and saw the Eden Cool+ and wanted to give it a try. I figured it could only be better than the original Eden…but it is not. While the outer cover is noticeably cooler to the touch, it is not soft like the Eden. Also, this cool material mostly gets negated if you use a pillow case anyway. The shaped fill material I would also say is “lumpier” for lack of a better word than the shredded material in the original. The combination of the harder, less supple outer casing and the lumpy material just aren’t a good combination. I will say it does sleep slightly cooler than the original Eden but not enough for the sacrifice in comfort. I would absolutely consider going back to the original Eden.
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Reviewed in the United States on February 26, 2026
★★★★★ 5
Unmatched Pillow with Unmatched Customer Service!!! 5 STAR ALL AROUND
Team Name: Eden Cool+, Size: King, Color: Classic
## A Dream Come True (and Customer Service to Match!)
From the moment I first laid my head on the Coop Eden Cool+ pillow, I knew I'd found something special. It's truly a game-changer for hot sleepers like me. The "Cool+" technology isn't just a gimmick; it actually works to keep my head and neck comfortably cool throughout the night, leading to the most refreshing sleep I've had in years. The adjustable fill is brilliant, allowing me to customize the loft perfectly for my sleep style, which means no more waking up with neck pain. The support is fantastic – it cradles my head without feeling too firm, and it holds its shape beautifully.
However, what truly elevates my experience to a full five stars, beyond the exceptional product itself, is the **dedicated and outstanding customer service**. I had a minor issue with my initial pillow (a rare manufacturing defect, which can happen with any product). I reached out to Coop Home Goods, and their response was nothing short of phenomenal. They were incredibly prompt, empathetic, and went above and beyond to resolve the issue swiftly and completely. They genuinely cared about my satisfaction and ensured I was left with a perfect product.
It's rare to find a company that not only produces a superior product but also stands behind it with such integrity and genuine concern for their customers. The Coop Eden Cool+ pillow is an investment in your sleep, and knowing that you're backed by such an incredible customer service team makes it an even easier decision. Highly, highly recommend!
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Reviewed in the United States on August 8, 2025
★★★★★ 5
Dream pillow
I’ve been using latex pillows for over 30 years, and I can’t find them in local stores anymore. I’ve been needing to replace my pillow and finally decided to try this one. There are so many options on Amazon that it’s overwhelming. But this standard low loft pillow ended up being PERFECT. It feels amazing and just like the pillows I’ve been using since childhood. It is soft but cradles my head and neck perfectly. For me, it’s like sleeping on a gentle cloud. Very happy with my purchase. (Yes, it does have a slight latex odor. That is normal due to the material. It’s not very strong and it doesn’t bother me).
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Reviewed in the United States on May 18, 2026