Pay in installments of $55.91 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Sep 10 - Sep 15
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TREM2 ELISA KitProduct Specification protein TREM2 Usage 1. Self brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent Ready to use; Before use, equilibrate to room temperature; 2 8 Save. Standard Diluent Used to dilute standard. 1Assay Buffer Before use,
Product Specification
| protein | TREM2 | ||||||||||||||||||||||||||||||||||||||||
| Usage |
1. Self-brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi-channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. Standard Diluent Used to dilute standard. 1×Assay Buffer ： Before use, equilibrate to room temperature and press with deionized water 1 ： 10 Proportional dilution of Assay Buffer (10×) get 1×Assay Buffer , mix gently to avoid foaming. 2-8℃ Preservation, this solution can be stable 30 Heaven. 1×Assay Buffer For diluting serum and plasma samples, HRP-conjugated Human TREM2 Detect Antibody (100×) 。 Human TREM2 Standard ： The label superscript added to the standard focuses on suspension volume reconstitution in deionized water Human TREM2 Standard get 20 ng/mL Standards. Before performing dilution, hold the standard at least 15 min And gently shake, after reconstitution Standard Available at -20℃ Save 1 Months, redissolved and used 1 Discard after times. 1×HRP-conjugated Human TREM2 Detect Antibody ： Mix thoroughly before dilution. Depending on the amount required for standard and sample, use in clean plastic tubes 1×Assay Buffer Right HRP-conjugated Human TREM2 Detect Antibody (100×) Conduct 1:100 Dilution. 1×HRP-conjugated Human TREM2 Detect Antibody Should be after dilution 30 min Internal use. HRP Substrate (TMB) ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Store in the dark from light. Stop Solution ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. 1×Wash Buffer: Equilibrate to room temperature before use, Wash Buffer (20×) Performed with deionized water 1:20 Dilution to obtain 1×Wash Buffer 。 Mix gently to avoid blistering, store at room temperature, this solution is stable 30 Heaven. Standard curve settings: As shown in the table below, use Standard Diluent Will 20 ng/mL The standard was diluted to 10 、 5 、 2.5 、 1.25 、 0.63 、 0.31 、 0.16 And 0 ng/mL Of Human TREM2 Standards.
Note: Each experiment requires a new formulation of standards. 3. Sample preparation: 1 Cell culture supernatant: centrifuge to remove particles, immediately measure or dispense -20℃ Store and avoid repeated freezing and thawing. 2 Serum: Use a serum separation tube and allow the sample to coagulate at room temperature 30 min , then 1,000 g Centrifugation 15 min 。 Take the upper layer serum for immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. 3 Plasma: use EDTA , heparin or citrate as an anticoagulant. After collection 30 min Inner 1,000 g Centrifugation 15 min 。 Immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. CAUTION: Do not use specimens with severe hemolysis or lipidemia. If the sample is to be in 24 h Used within, you can store it in 2 To 8℃ , avoid repeated freezing and thawing. Frozen samples should be slowly warmed to room temperature and gently mixed prior to determination. 4. Experimental steps: 1 Remove the excess slats from the plate frame, place them back in the aluminum foil bag containing the desiccant pack, and reseal them. 2 The liquid in each well was removed and washed. Add per well using a multi-channel pipette or automatic washer 300 μL 1×Wash buffer Let stand and soak 30 s , performing washing. Complete removal of the liquid in each step is essential to obtain good performance. After the last wash, the plate was inverted and blotted dry with a clean paper towel to remove any remaining 1×Wash Buffer 。 Use the plate immediately after the plate wash is completed and do not allow the plate to dry. 3 , Add standard: add standard well 100 μL Diluted standards. 4 Add sample: serum / Plasma: sample well addition 80 μL 1×Assay Buffer And 20 μL Pre-diluted samples; Cell culture supernatant: sample well addition 100 μL Cell culture supernatant (See sample preparation for details). 5 , add detection resistance, add each well 50 μL 1×HRP-conjugated Human TREM2 Detect Antibody , guarantee step 3 、 4 、 5 Add samples continuously without interruption. The sample loading process is 15 min Within completion. Plate was coated and incubated at room temperature 2 h 。 6 Repeat steps 2 Washing in 6 Times. 7 , adding per well 100 μL HRP Substrate (TMB) 。 The plate was coated and incubated at room temperature protected from light 5-30 min 。 8 , adding per well 100 μL Stop Solution ， Stop Solution Should be in accordance with the TMB The same order is added to the plate. The color in the hole should change from blue to yellow. If the color in the hole is green or the color changes unevenly, tap the plate lightly to ensure thorough mixing. 9 , in 30 min Within, measure each well 450 nm The absorbance value at. Note: 5-30 min The color development time is within the empirical range. For each specific experiment, the approximate color development time can be determined according to the following conditions. （ 1 ) Visual observation: standard song S5 The holes are light blue, Blank When the hole has no obvious blue color, it can be terminated; （ 2 ) Instrument judgment: 630 nm At left and right wavelengths, standard curve S1 Porous OD Value reaches 0.5 - 0.7 、 S5 Porous OD Value reaches 0.05 - 0.08 、 Blank Porous OD Value less than 0.05 It can be terminated. 5. Result calculation: 1 Calculate the multiwell average of each standard and sample OD Value, and subtract the zero concentration ( Std.8 ) average OD Value. 2 , Drawing of standard curve: take the standard concentration as x Axis, the average absorbance of each standard is y Axis, plot the standard curve. You can use mapping software to create standard curves. Note: If the sample is diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 6. Results display: Typical standard curve ( R2≥0.99 ） Figure1. 96 Well plate analytical Human TREM2 Standard curve. The data and curves are for reference only, and experimenters need to establish standard curves according to their own experiments. |
||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 0.16 ng/mL | ||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||||||||||||||||||
| Synonym | Human TREM-2 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||
| Detection Type | Sandwich method | ||||||||||||||||||||||||||||||||||||||||
| Description | Myeloid cell trigger receptor 2 (TREM2) is a single Ig domain receptor that is expressed in macrophages and dendritic cells, but not in granulocytes or monocytes. It is most abundantly expressed in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, while microglia in the central nervous system are the most dominant cell type producing TREM2. TREM2 plays a role in chronic inflammation and can stimulate the production of constituent rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP to activate the immune response of macrophages and dendritic cells. It is also associated with the adaptor protein DAP12 to transmit activation signals, which play a role in innate and adaptive immune responses. TREM2 Signaling is also an important way to promote colonic wound healing. TREM2 mutations increase the risk of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease. The Human Myeloid Cell Trigger Receptor 2 ELISA Quantification Kit employs a double antibody sandwich method to quantify human myeloid cell trigger receptor 2 in a sample. The plate is coated with an antibody specific to human myeloid cell trigger receptor 2, and the sample or standard and the specific detection antibody labeled with HRP are added to the wells of the plate. The human myeloid cell trigger receptor 2 binds to the solid phase antibody and the detection antibody coated on the plate. After washing, a chromogenic substrate (TMB) was added, and the TMB became blue under HRP catalysis and turned yellow after addition of the stop solution. The OD value was measured with a microplate reader at 450 nm wavelength, and the concentration of human myeloid cell trigger receptor 2 was directly proportional to the OD450 value. |
||||||||||||||||||||||||||||||||||||||||
| Composition |
|
||||||||||||||||||||||||||||||||||||||||
| General Notes | 1. If Standard Diluent and Assay Buffer (10 ×) turn yellow in color and have a small amount of precipitation, it is because the reagent contains serum. Just centrifuge to remove the precipitation, which will not affect normal use. 2. Do not mix components with different batch numbers and different manufacturers; Failure to do so may lead to abnormal results. 3. Change the tip frequently to avoid cross-contamination between the components. 4. To ensure accurate results, the plate must be properly adhered during the incubation step. 5. Stop Solution is corrosive to a certain extent, so please take protective measures when operating. |
||||||||||||||||||||||||||||||||||||||||
| Instructions | Serum (plasma), cell culture supernatant | ||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | 2-8 ℃, protected from light, valid for 24 months. | ||||||||||||||||||||||||||||||||||||||||
| Test Range | 0.16 ng/mL-10 ng/mL |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 25 reviews
Sort
Product Reviews
★★★★★ 3
Not indestructible but pretty darn tuff for sure!
Size: Large, Color: Blue
I would have maybe given a better rating if they hadn’t lied about it being indestructible and recommended it for my dogs size range! It didn’t last the first day. However my dog may be a bit above average when it comes to his relationship with the balls! It’s an issue and he should probably be in a 12 step program of some kind. But we all have our vices right and his is balls. All of them he needs em and he never stops joining for them and the stronger they are the more he’s driven to defeat it! So now that you have full disclosure from me. The ball is still in his box and it’s still round and mostly intact and that’s after several months! The part that didn’t last the first day was the squeaker! Poor little dude! If he would have just stayed quite it. But he didn’t and Bo dog knew he was in there! And made short work of him. Other than that. The ball pretty dang tough! But it’s not indestructible is it a big deal. Well in this world of way to many choices and then having to find the few that will tell you honestly what your getting and are not over stating their product! Just to get your money. And couldn’t care less about the quality! It sucks that we have to navigate all the crap to find the few decent things available. So it most certainly does matter! And I say that for their benefit the most hopefully! Because people are craving and constantly looking for a company that just makes the effort to put out the best they can. And tell you the honest description of what they have. That stands behind their work and puts more effort into quality then they do into profit. With the faith that the quality and integrity in their company will end up bring them the profits they can be proud of and know that they earned! Honestly! Is this company one of those! I don’t know? It’s not clear and in their defense I never reported the sneaky man’s demise on day one! I expected that. But indestructible is a descriptive word that has a very specific meaning. And when it’s used in a manner that it was! Then you’ve set yourself up to fail as there are very few things that meet that description! And I know I’m picking hairs here. But it’s words that get used like that wich makes one appear to have not met the standard they wanted to! And maybe you don’t realize its impact on your overall until it’s far down the road! Can it be said better. Sure . Always room for improvement. But I would certainly remove the claim that’s just unreasonable to expect. If I wanted to use the benifit the does have to offer perhaps I would have said this ball is the closest thing to indestructible we’ve made so far. That’s statement tell me that they are not claiming it is but that it’s pretty tough! And it with out exact words tells you these guys must take honesty seriously or they wouldn’t have chosen to claim the accurate step below. And that lets me know that they hold themselves to a standard that they obviously respect their customers and they don’t take them for granted that’s integrity right there and there’s just not enough of it o. This world today! Seems to me that there are a few that are really close to it though. Maybe so close it’s worth the time to just go ahead and say it. If my gut feeling is right then it’s absolutely worth the time! Do I recommend the item absolutely it’s as tough as nails! And if you hate the squeaky part. Then don’t worry it won’t last long if your dogs like mine. Or you can take the little dude out when the dogs sleeping! And I’m going to add a star because when I look at it in the wider view I’ve been a bit harder then is justified I believe. Their. Our lives today are not as good as they once were and should be! Because of our bad habit of not holding ourselves to the standards we are capable of and rather settling for what’s out there at the time. Because of many reasons right. What’s the point. Or it’s not going to change a thing. Or that as good as it needs to be. Don’t want to seem so picky and all the justification we inject in the place of our potential. That’s had a vey negative effect across the board! And we’re losing so much more then quality dog toys as a result. When we let or standards drop due to whatever reasons as a civilization then are standards drop. And that’s all you should need to know. To understand where that leads! And we only do ourselves the disservice and loss that comes from those choices. So look around and don’t hesitate to. All out that when you see it. Just try and come from a place that’s not to tear things down but to remind them of what they are really capable of! And how much we all need to hold ourselves to the highest standard we are capable of! And stop justifying excepting less! And then we see the results from that rather than the other.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 18, 2025
★★★★★ 5
MUST BUY! For large Dogs Safe & Fun! 100lb Dutch shepherd loves this
Size: Large, Color: Blue, Size: Large, Color: Blue
I have 100 pound Dutch Shepherd who likes to chew and he goes through toys pretty quickly. This one he has been chewing on for hours and has not made a dent, not one!! he loves it. He bounces it. It’s very bouncy. I love that about it the engagement with it is great! noise level. It makes some noise. I’ve got wood floors so you can hear it bouncing and the squeak is pretty loud, which is fine with me. It doesn’t bother me. My pet really really enjoys this a lot. I think this is an amazing value for the money because I’ve spent more money on other toys and they fell apart within a day. I think it’s a safe toy because he’s not got a little hard plastic pieces that are coming off of it. It does not break apart very easily and the size is good. it fits in the palm of my hand so it’s pretty good size ball for him. It’s big enough. He cannot swallow it, but he can fit it in his mouth. I think the thing that I like the most about it and he enjoys is that when he drops it, it bounces and he can chase it. Pet enjoyment 10 out of 10 engagement 10 out of 10. I feel it’s very safe dog friendly absolutely must buy hands-down. You will not be disappointed. It’s made of a very strong. I would say rubber and it’s super easy to clean. Just toss it in the sink. Wash it off with soap and water rinse it. It’s good to go. I’m running to buy another one right now as we speak so just in case he loses it, he’ll have a backup.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
Great, solid toy safe for my dog
Size: Large, Color: Blue, Size: Large, Color: Blue
This ball is great! My pit bull love toys, especially ones with squeakers, but she’s a power chewer and trashes them almost immediately. Since she’s got a history of swallowing foreign bodies, this means she’s rarely been able to have toys, and when she does get them, it’s short lived. She LOVES this ball and it’s already lasted multiple weeks with no signs of any damage, despite the fact she carries it everywhere and chews on it daily. We don’t have to worry about her safety with this toy, because it’s large and tough enough she can’t swallow anything. It hasn’t lost its squeak either despite all her tough love. She loves to play with it and we’re getting more of them asap so she has a backup if this one gets lost or eventually does break.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
★★★★★ 5
Truly indestructible
Size: Large, Color: Blue
They are not kidding, this ball is truly indestructible. I've gotten several for my dogs so they have plenty to share. Two of my dogs are beyond super chewers (bully breeds). One of the balls is missing the section that's glued into most of them, but the structure has still held up. And my dogs LOVE them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2026
★★★★★ 4
Tough STINKY ball
Size: Large, Color: Red
This ball is the only one I've found that my dog can not destroy in seconds. He can chew on it for a few weeks before he works a hole in it. That's the good part. The bad side is the horrible smell! It doesn't seem to bother him, but it is horrible to my nose. It is so awful that I tried soaking it and dish soap and vinegar water overnight to see if I could make it go away. It was a little bit better but still stunk. Anybody got other suggestions on what could make the smell go away?
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026
recommand products
95-00 Lexus UCF20 LS400 Front Right Passenger Side Door Shell Panel Black OEM
128.65
07-15 Jaguar X150 XK XKR Convertible Rear Left Side Quarter Window Glass OEM
53.95
07-13 BMW X5 E70 Front Right & Left Engine Bay Protection Bracket Mount Set OEM
41.50
10-15 Jaguar XK X150 5.0L N/A Rear Back Bumper Lower Extension Cover C2P1668 OEM
87.95
Worcester Massachusetts Colorful City Map Canvas Print
76.00