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Description
Human TNFAIP8L2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tip: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: serum, plasma, tissue, homogenates, cell culture, supernatants and other biological fluids Preparation before testing: 1. Please take out the test kit from
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tip: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: serum, plasma, tissue, homogenates, cell culture, supernatants and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against TNF alpha-induced protein 8 like 2 (TNFAIP8L2). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of TNF alpha-induced protein 8 like 2 (TNFAIP8L2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TNF alpha induced protein 8 like 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TNFα-induced protein 8-like 2 (TNFAIP8L2), also known as TIPE2, is a protein encoded by the TNFAIP8L2 gene. It is preferentially expressed in myeloid cell types and acts as an immune checkpoint regulator of inflammation and metabolism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids |
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4.1 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 5
Nice long sleeve Oxford shirt
Fit Type: Slim Fit, Color: White, Size: Medium
Exactly what I expected plus fast delivery. Amazon essentials offer great value for the price.
Note: I wish #Amazon would give points for reviewing items as an incentive just as #AliExpress does.
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Count on shrinkage when choosing your size.
Fit Type: Slim Fit, Color: White, Size: X-Large
I bought this shirt earlier and got an XL. It came and it fit perfectly. Couldn't believe it, but then I wondered what it would look like after one washing. It shrank. Not so bad that I couldn't wear it, but it no longer looked "perfect" and I I don't dare put on 5 pounds. But I liked the shirt overall, so I went back and ordered another, this time an XXL. It came, I tried it on and while it worked, it was slightly larger than I wanted. Instead of returning it, I washed it, and like the first, it shrunk, but it shrunk to a size that looked good and was comfortable. Lesson: you might want to go one size larger than you would ordinarily get, wear it to see how it fits, and if it seems too large, wash it and see if it makes a difference. The first shirt I would give 4 stars because shrinkage took away from it, but I'd give the second 5 stars because even more so after washing, the fit was very nice.
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Reviewed in the United States on April 23, 2025
★★★★★ 3
Good concept, details need some work.
Fit Type: Slim Fit, Color: Grey, Size: Small
Let's start with the positives. The quality of the cloth is great, it's a heavier cotton, but feels soft and durable. The overall slim fit cut was great too. The shoulders were a tiny bit too boxy for me personally, but I didn't wash it to see if the shirt would shrink and solve that issue.
The problem with this shirt is that it doesn't get the details right. It's almost like they just took a regular shirt and decided to produce it in a slim fit instead, without tweaking anything else. It's slightly too long to be left untucked, and for a cloth this heavy, it's not really something that you would really want to wear tucked in. I also find it weird that a slim fit shirt would have a box pleat on the back, since the whole point of a slim fit shirt is for a cleaner look on a trimmer guy. Finally, I like the button-down collar, but would consider hiding the buttons on the back of the collar so that you get a cleaner look. Unfortunately, these misses on the details make this shirt seem outdated. Other than the slim cut, it reminds me of something you might see from the 1990s, along with double pleated chinos.
Overall, doesn't seem like a good match for a younger, more stylish demographic that might want to wear a slim fit cut. Despite the great price, I'm definitely returning this one.
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Reviewed in the United States on January 31, 2019
★★★★★ 5
Very nice winter shirt.
Fit Type: Slim Fit, Color: Blue, Size: Large
These shirts are beyond what I expected. They’re thicker than I thought but not being too heavy. They wear well and they also wash up very well in permanent press and I dry them on permanent press also. No problem with wrinkles. I just take them out of the dryer and hang them up and they look great.
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Reviewed in the United States on December 14, 2025
★★★★★ 4
Amazon Essentials Mens shirt review - Good shirt, great value.
Fit Type: Slim Fit, Color: Grey, Size: X-Large
First, this is Oxford. This is a tough fabric that looks a little like pinpoint and feels a little like flannel. That is, it does not have the sheen of a dress shirt and is not as thick and soft as flannel. It hits a nice spot in between. The fabric is soft enough and thick enough to wear casually in the heat of summer. (It is cooler than a very thin and very cheap shirt due to it's absorbancy.) The fabric appears and feels to be quality.
The cut is good for me. It is tailored enough to look good tucked in and roomy enough for work and play.
The seams are doubled and good quality. I did not find mid seam knots, broken threads, etc. Seams are likely very durable.
The Goodthreads shirt is a little more expensive. It is a little thicker and softer. It is almost a flannel. However, the thickness and softness caused it to shrink more in the dryer. It loosened OK as I wore it, but I think after multiple washings and dryings, the Goodthreads may get a little tight. I have no concerns with the Essentials.
Good shirt.
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Reviewed in the United States on August 8, 2020