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Description
Hyaluronidase PH20/SPAM1, Human (GMP)Product Specification Species Human Synonyms Sperm adhesion molecule 1, SPAM1, Vorhyaluronidase alfa, rHuPH20 Accession P38567 Amino Acid Sequence Leu36 Tyr482 Expression System CHO Host cell protein 100ppm Host cell DNA 100ppb Mycoplasma Sterility phValue 6. 0 6. 4 ProteinContent 0. 28 mg ml SdsPageMolecularWeight 60 70 kD SdsPagePurity >97% SecHplcPurity >97% CellActivity 3. 6104U mg Conjugation Unconjugated Tag No Tag Physical Appearance Liquid
Product Specification
| Species | Human |
| Synonyms | Sperm adhesion molecule 1, SPAM1, Vorhyaluronidase alfa, rHuPH20 |
| Accession | P38567 |
| Amino Acid Sequence | Leu36-Tyr482 |
| Expression System | CHO |
| Host cell protein | ＜100ppm |
| Host cell DNA | ＜100ppb |
| Mycoplasma | 阴性 |
| Sterility | 符合 |
| phValue | 6.0-6.4 |
| ProteinContent | 0.28 mg/ml |
| SdsPageMolecularWeight | 60-70 kD（还原） |
| SdsPagePurity | >97%（非还原） |
| SecHplcPurity | >97% |
| CellActivity | 比活：3.6×104U/mg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 150mM sodium chloride, 10mM dibasic sodium phosphate, 15mM albumin human, 10mM L-methionine, 0.02% polysorbate 80, pH6.2±0.1 |
| Stability & Storage | · 12 months from date of receipt, liquid stored at -20 to -80℃. |
| Reference | Active Expression of Human Hyaluronidase PH20 and Characterization of Its Hydrolysis Pattern. Bo Pang; Jing He; Weijiao Zhang; Hao Huang; Yang Wang; Miao Wang; Guocheng Du; Zhen Kang; Front Bioeng Biotechnol. 2022 May 13:10:885888; |
Background
Hyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). There are six known types (hyaluronidase 1–4, PH-20, and HYALP1). Human hyaluronidase is present both in organs (testis, spleen, skin, eyes, liver, kidneys, uterus, and placenta) and body fluids (tears, blood, and semen). Testicular PH20 hyaluronidase is found on the surface of human sperm and inner acrosomal membrane and degrades hyaluronic acid in the ovum during fertilization. Hyaluronidase PH20 is known as sperm adhesion molecule 1 (SPAM1) and random hydrolysis of (1, 4)-linkages between N-acetyl- beta-D-glucosamine and D-glucuronate residues in hyaluronic acid (HA). PH20/SPAM1 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. PH20/SPAM1 protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
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