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Description
Human EPB42 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Erythrocyte Membrane Protein Band 4.2 (EPB42) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Erythrocyte Membrane Protein Band 4.2 (EPB42) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Erythrocyte Membrane Protein Band 4.2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Erythrocyte membrane protein band 4.2, also known as EPB42, is a protein encoded by the EPB42 gene. It is part of the erythrocyte cytoskeleton. It is an ATP-binding protein that regulates the binding of band 3 to acetylcholine. It may play a role in regulating erythrocyte shape and mechanical properties. Mutations in this gene are associated with recessive spherocytosis and recessive hereditary hemolytic anemia. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Soft and comfy
Color: Green, Size: Twin, Color: Green, Size: Twin
This mattress pad is much thicker and fluffier than the one I had before. With I took it out of the package, it was vacuum sealed with a note saying to leave it out in the sun for 48-72 hours or fluff it up with a hair dryer. I put it in the dryer on medium heat, which worked nicely. The pockets are really deep on this mattress pad and it fits snug. You don’t really see the shape with a sheet on top. It feels comfortable laying on top of it. It doesn’t flatten much when I’m laying down, but I imagine over time it will lose fluffiness. Overall it’s comfortable and breathable.
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Reviewed in the United States on May 31, 2026
★★★★★ 2
Good for already soft mattresses, 1in thickness.
Color: Dark Grey, Size: Queen
If your mattress is already soft, it will help. It's less than 1in thick after tumbling for 40 minutes. It did fit my queen bed and felt somewhat soft when laying down but it didn't help much as far as comfort. Quality wise it looks good, not cheap and while my sleep quality wasn't improved, it might help someone with a better mattress than mine.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Must have for mattress comfort and protection
Color: Green, Size: Twin, Color: Green, Size: Twin
I ordered the BDEUS Twin mattress topper for a temporary mattress. The topper definitely improves comfort and regulates temperature much better than just the mattress and sheets. The cover fits deep mattress easily fitting over my 13” mattress and could easily fit a much deeper bed. The padding is thick enough to provide some additional support. This model also has a thin sheet of waterproof material to help protect your mattress against spills or liquid damage. This layer is cover by the pillow top and does not seem to make much if any noticeable noise. Overall this is a high quality cover and mattress protector in one. At <$45 at the time of this review it provides an incredible value.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Topper
Color: Green, Size: Twin
I recently purchased the BDEUS Twin Mattress Topper Viscose Derived from Bamboo, and I am thoroughly impressed with my experience.
The comfort level is absolutely a 5 out of 5. The viscose bamboo material feels soft and plush, making my bed significantly more comfortable to sleep on. I wake up feeling well-rested and supported, which has been a noticeable improvement from before.
Here are some of the main pros and cons:
Pros: Incredible comfort and softness, high-quality materials, fits the twin mattress perfectly, and helps with temperature regulation throughout the night.
Cons: Name
Overall, I give the BDEUS Twin Mattress Topper a solid 5 out of 5 stars. It has completely transformed my sleeping experience, and I would highly recommend it to anyone looking to enhance the comfort of their bed.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Adds comfort and support
Color: Green, Size: Twin
I’ve been using this bamboo mattress topper for a couple of weeks now, and it completely changed the feel of my bed. My mattress was starting to feel too firm and uncomfortable, especially on my lower back and shoulders, but this topper added the perfect amount of cushioning without making the bed feel overly soft or saggy.
The cooling effect was one of the biggest surprises. I tend to sleep hot and usually wake up sweating during the night, but the bamboo-derived fabric feels breathable and much cooler than regular mattress pads I’ve owned before. It stays comfortable throughout the night and doesn’t trap heat like memory foam toppers sometimes do.
I also really appreciate the waterproof protection. It gives peace of mind without feeling stiff or making that annoying crinkling noise some waterproof covers have. The surface still feels soft, plush, and smooth. It honestly feels more like a hotel-style pillow top than a basic mattress pad.
The thickness is just right for pressure relief. It helped reduce the soreness I usually wake up with in my back, and I’ve been sleeping much more comfortably overall. The deep pockets also held securely on my twin mattress and didn’t slide around, even after moving a lot during sleep.
Another thing I liked was how fluffy and well-made it looked right out of the package. After letting it expand for a bit, it became nicely plush and evenly padded. The stitching seems durable, and it still looks great after washing.
If you’re looking for a softer, cooler sleeping surface with added waterproof protection and better support for aches and pains, this mattress topper is definitely worth it. It made my bed feel newer and far more comfortable without needing to buy a whole new mattress.
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Reviewed in the United States on May 14, 2026
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