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Description
Magnetic Bead-Based Immunoprecipitation (IP) KitProduct Specification Application IP, Co IP Physical Appearance Liquid Stability & Storage 12 months from date of receipt, 2 to 8 C as supplied Background Immunoprecipitation (IP) is a technique based on the specific antigen antibody binding reaction. The magnetic beads in this kit are covalently conjugated with protein A G, which can specifically bind to the Fc region of immunoglobulins (IgG) from various species. The workflow involves the following
Product Specification
| Application | IP, Co-IP |
| Physical Appearance | Liquid |
| Stability & Storage | 12 months from date of receipt, 2 to 8 °C as supplied |
Background
Immunoprecipitation (IP) is a technique based on the specific antigen-antibody binding reaction. The magnetic beads in this kit are covalently conjugated with protein A/G, which can specifically bind to the Fc region of immunoglobulins (IgG) from various species. The workflow involves the following key steps: 1) Add protein A/G- magnetic beads to capture antibody through the interaction between protein A/G and the antibody's Fc region; 2) Incubate the sample (containing the target antigen) with the beads-antibody complex to form beads-antigen-antibody complexes; 3) Wash the beads to remove non-specifically bound impurities; 4) Elute the target antigen (or antigen-antibody complex) from the beads using either acidic or denaturing elution buffer; 5) Collect the eluted sample for subsequent experimental analysis.
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Exchange/Return Notes
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