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Description
Human RRP8 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against ribosomal RNA-processing protein 8 (RRP8). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of ribosomal RNA-processing protein 8 (RRP8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ribosomal RNA-processing protein 8 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Ribosomal RNA processing protein 8 is a protein encoded by the RRP8 gene. Diseases associated with RRP8 include essential tremor and spermatogenesis failure. 65 Its pathways include activating PKN1, stimulating transcription and chromatin regulation/acetylation of AR (androgen receptor)-regulated genes KLK2 and KLK3. Gene Ontology (GO) annotations associated with this gene include RNA binding and methylated histone binding. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.5 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Five star
I love this product. It works well with my skin
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 14, 2026
★★★★★ 4
Pros and cons at once
It smooths and brightens my skin, but it’s not very moisturizing, so I have to reapply it frequently. Expensive too
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Reviewed in the United States on March 11, 2026
★★★★★ 5
Nice
Love it
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Reviewed in the United States on April 20, 2026
★★★★★ 5
Excellent choice for a gentle daily lotion
I have dry, mature skin, so I’m always looking for a lotion that actually hydrates without feeling heavy or irritating. I’ve been using the Koji White Body Lotion and it’s been a really nice addition to my routine. The formula feels lightweight but still moisturizing enough for my dry skin. It absorbs quickly and doesn’t leave a sticky or greasy film, which I really appreciate.
I like that it contains kojic acid and papaya extract. Over time my skin has looked a bit smoother and more even, and it definitely feels softer after applying. The lotion is pH balanced and free from parabens and artificial dyes, which makes it gentle enough for everyday use on my more mature, sensitive skin.
Another thing I enjoy is the light, pleasant scent, it’s subtle and fresh without being overpowering. Overall, it keeps my skin feeling hydrated, nourished, and comfortable throughout the day, and I’ve had no irritation. It’s a good daily lotion if you want something moisturizing with skin brightening ingredients that still feels gentle.
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Reviewed in the United States on March 7, 2026
★★★★★ 5
Silky, Effective Body Lotion With Strong Scent
In the winter, my skin is prone to becoming very dry and flaky. I ordered this "Kojic Acid & Papaya Body Lotion - Exfoliating, Hydrating, Moisturizing, Skin Care for Sun-Damaged Skin" by Koji White hoping it could help keep my skin from feeling dry and itchy. I ordered this lotion in mid Dec 2025, and the bottle is labeled with a manufacturing date in late Oct 2025 and an expiration date of Oct 2028. I’d consider this a very fresh product. The pump works smoothly, and I didn’t have any trouble getting it to dispense lotion. The label lists Thailand as the country of manufacture. I also liked that the listing describes the product as vegan and free of animal testing. I’ve been vegetarian for about 30 years, so this matters to me.
I’m aroma sensitive, and this lotion lists fragrance as one of the ingredients, which is always a gamble for me. I definitely detect tropical fruity notes, and papaya is listed in both the name and ingredient list. There’s also a noticeable floral scent. I don’t usually like floral fragrances, but the fruity and floral scents complement each other fairly well. Unfortunately, the scent is much stronger than I prefer and seems to linger.
I really like how this lotion feels on my skin. It’s a little less creamy than I expected given how hydrating it is, but it applies smoothly and evenly. It absorbs quickly without leaving my skin feeling sticky or greasy. My skin feels hydrated and silky smooth after applying, and it’s made a noticeable difference in how the skin on my legs looks and feels. Unfortunately, I’ve only been able to use it on my legs when I’m wearing pants because the fragrance is too intense for me. I love the results and wish I could comfortably use it all over.
Overall, I really like this lotion. It has everything I look for to help prevent dry winter skin. The only drawback for me is that it’s more heavily scented than I prefer. At the time of my order, the 250 ml (8.45 oz) bottle cost $20. Given the quality and performance, that feels like a good value. For people who enjoy more strongly scented products, this lotion could be an excellent choice. Since fragrance preference is so personal, I won’t be deducting any stars from my rating.
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Reviewed in the United States on January 1, 2026