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Description
Mouse monoamine oxidase,MAO ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with monoamine oxidase and MAO capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the monoamine oxidase and MAO content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse monoamine oxidase,MAO ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Monoamine oxidases (MAO) are a family of enzymes that catalyze the oxidation of monoamines, using oxygen to remove their amine groups. They are found bound to the outer mitochondrial membrane of most cell types in the body. The first such enzyme was discovered in the liver by Mary Bernheim in 1928 and named tyramine oxidase. MAO belongs to a family of proteins called flavinamine oxidoreductases. They are important in breaking down monoamines ingested through food and also play a role in inactivating monoamine neurotransmitters. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.6 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Lashes like Garbo
Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer, Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer
I think this might be my new go to mascara.
I have the teeniest tiniest lashes ever, they are straight and stick out from my eyelids and without mascara I look like I have no lashes so it’s a struggle finding mascaras that make my lashes look lush.
Let me start off by saying that the primer is amazing.
As soon as I put it on my lashes grew over twice in length!
It didn’t clump and separated my lashes like whoa( the clumping with these lash fiber or extra lengthening mascaras is always horrendous so that was a wonderful surprise), it dried in about 20 seconds and then I put on the coal black mascara.
Vavoom ladies!
Instant fabulous lashes.
So far I have experienced zero flaking after wearing for long periods of time , it hasn’t smudged or melted when I’m dancing up a storm or getting my workout on and the length of my lashes is practically miraculous.
It is not cry proof.
It’s super easy to remove and doesn’t tear out any of my eyelashes though, so that’s another plus!
Also, I have celiac disease and a host of other random allergies and am allergic to SO MANY products it’s ridiculous, but luckily I am not allergic to this magic mascara.
I recently discovered that this doesn’t smudge on my bottom lashes either!
I’m almost 40 and I have never found a mascara that didn’t smudge on my bottom lashes. I’m so oily that even waterproof ones didn’t work!
I stopped wearing it on my bottom lashes for anything but photo shoots after 20yrs old because the smudging made me look like an old lady with terrible makeup skills,but now I can once again rock liner on my bottom lashes!
I included a terrible picture ( I hate getting my picture taken!) and tried to crop it so you can get an idea of how amazing this mascara is( look at those lashes practically touching my brow bone!),because I know I always want to actually see the results of people using the product, so hopefully it helps. :-)
Ps: I don’t have mascara on my bottom lashes in the pic as I didn’t discover it was even a possibility for me until I bought a tube for my bestie ( another oily faced queen)and she told me there was no smudging:-O
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Reviewed in the United States on March 21, 2019
★★★★★ 5
Great mascara, my new favorite!
Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer
I think this is my favorite mascara of all time! lol!
Goes on very easy and not clumping. Makes my eyelashes look longer.
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Reviewed in the United States on June 3, 2026
★★★★★ 4
Mascara is good, but don’t expect the best - smudges & flakes sometimes
Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer
This mascara is very good for lengthening when you use the primer. I’m not sure I’ve noticed a volume/thickness increase, but have noticed lash length does look longer. I usually give it about 30 seconds for the primer to dry before using the mascara, otherwise it does clump a bit. I would also recommend wiggling both wands along your lashes during application to avoid clumps. In general I do like this, but noticed a few other important points: it will definitely run a lot if you get it wet (e.g. rain or crying), it will flake or smudge a little by the end of the day - especially if you rub your eyes, and while it does not make my eyes itch, I found it best to put in my contacts AFTER the mascara is fully dry. I’ve accidentally got some mascara a few times in my eye with the wand/trying to smooth out clumps and it totally made my contacts (daily wear type) useless after that. That all probably sounds like the mascara isn’t worth it, however I still actually like this mascara. I’d say it’s perfect for an average day’s wear, just not an extended day or in some cases. It does come off fully for me.
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Reviewed in the United States on December 31, 2022
★★★★★ 5
My Go-To Mascara for Years — Makes My Lashes Look Fuller and Longer!
Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer
I’ve been using this mascara brand for a few years now, and it’s never let me down. This particular two-in-one (primer + mascara) combo works so well — it gives my lashes a great boost and really helps them look fuller and longer over time with consistent use.
Every time I wear it, I genuinely feel more confident and put-together — it just makes me look prettier, and that’s always a plus! The primer adds volume and helps the mascara go on smoothly without clumps. It lasts well throughout the day, too.
If you’re looking for a reliable mascara that delivers results and enhances your natural lashes, I definitely recommend this one!
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Reviewed in the United States on August 5, 2025
★★★★★ 3
okay so far
Size: 0.27 Fl Oz (Pack of 1), Style: Mascara/Lash Primer
bought this because i have recently developed a sensitivity to heavy makeup around the eyes. loved the ingredients of this mascara and rates a 3 on the think dirty app (amazing app check it out!) that being said, i honestly didn’t expect any crazy results like i would with my prior mascaras (l’oréal lash paradise and maybelline lash sensational) and i was right, it took forever for me to apply a decent amount to my lashes.
both sides seemed kind of dried out too, not the thick consistency that people were talking about in the reviews. the primer was dry and sort of settled in clumps around my lashes and i did the best i could to comb them out but i ended out plucking out the clumps with my finger tips. the mascara itself was very thin but no clumping, just felt dried out like i said. my lashes fell straight in a matter of half an hour.
it also flaked throughout the day and i spent it entirely indoors. i rubbed my eye once or twice and my inner corner felt a little irritated but im not sure it’s because of the product itself. the packaging itself is a little bulky when applying but i guess it’s a matter of working with it. i think it’s a little pricey for what it is but i think i can work with it, given that i don’t want harsh chemicals around my eyes. i will continue to be on the hunt for a “clean” mascara that works better though
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Reviewed in the United States on August 15, 2021