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Description
Human JTB ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Jumping Translocation Breakpoint (JTB) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the presence of Jumping Translocation Breakpoint (JTB) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Jumping Translocation Breakpoint ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Jumping translocation breakpoint protein (JTB), also known as prostate androgen-regulated protein (PAR), is a protein encoded by the JTB gene. It is an orphan receptor of unknown function. The JTB family of proteins includes several jumping translocation breakpoint proteins, or JTBs. A jumping translocation (JT) is an unbalanced translocation involving an amplified chromosome segment that jumps to various telomeres. It is essential for normal cytokinesis during mitosis and plays a role in regulating cell proliferation. It may be a component of the chromosome passenger complex (CPC), a key regulator of mitosis. The CPC complex has essential functions at centromeres to ensure proper chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Its overexpression causes mitochondrial swelling and reduces mitochondrial membrane potential. It has been linked to prostate cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Quiet, that's about the only good quality
UPDATE: Moving to 5 stars! Quickly after my 2 star review (below) the seller reached out and askked to exchange the defective product for an upgraded model at no cost to me. They were attentive and quick in responding, and I received a replacement version that also included the mopping functionality (The first didn't have this), and it works as expected. Customer service was too notch. I wouldn't hesitate to buy again.
This is a lot quieter than my original Roomba, that is still running strong...but I wanted a 2nd robot vac so I could run one on the main floor and 2nd floor of my home. This one doesn't fit the bill, and I will be returning. I ran it while I was at home, and it stopped 4 times, telling me I needed to clean the brush. There was absolutely nothing bound on the brush any of the times. But had I not been home, it wouldn't have finished cleaning because it stops completely when it thinks there is hair. Then, another time, it just stopped running. It didn't say anything, so I don't know why it stopped. I assume it thought it was done, but the whole level of my home wasn't finished. This was all in just the one time I attempted to run it for 1 cycle. When I pushed the start button again, it said something to the effect of starting cleaning, so that's what makes me assume the last cycle was over. It does clean well, and is very quiet; however, it just stops for no reason far too often. I want it to run while I'm at the office, which obviously won't work if I have to be here babysitting it for the imaginary dirt on the brush. This will unfortunately be getting returned. I think I'll stick to the Roomba brand, as I have far less issues with my 10+ year old vac than this new one with all the nice features.
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Reviewed in the United States on March 4, 2025
★★★★★ 3
Decent robot vacuum, but struggles with dog hair
The setup was straightforward and the self-empty station is a nice feature. It navigates the house fairly well and does a decent job picking up everyday dust and debris on hard floors.
Where it struggles is with dog hair. With a shedding dog in the house, I noticed it tends to leave behind quite a bit of hair, especially along edges and in areas where it tends to accumulate. It works better for lighter debris but doesn’t seem powerful enough to keep up with heavier pet hair.
Pros:
Easy setup and app integration
Self-empty station is convenient
Navigates rooms fairly well
Cons:
Struggles with dog hair
Not as strong as expected for pet households
Bottom Line:
This vacuum works fine for general cleaning and light debris, but if you have a dog that sheds a lot, it may not keep up as well as expected. For pet owners specifically, it might not be the best fit.
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Reviewed in the United States on March 11, 2026
★★★★★ 1
Very Disappointed with this Vacuum
After having a Shark Robot Vacuum for many years, my husband decided to upgrade to newer technology (easier boundary settings, better mapping etc)
On a good note, when this thing works - it works great. One of my biggest complaints with the Shark was I had to put physical boundary strips at the top of the stairs etc. This Eufy is very easy to set "no go zones" on the app, works famously in that aspect. This vac also seems to "work" in a more organized manner. I can see on the app exactly what was cleaned after every scheduled cleaning etc. The Shark would just buzz around the house all willy nilly, but this Eufy seems to be very organized in the cleaning, not missing anywhere. Now for the most aggravating part. I have my Eufy scheduled to clean every night @ 1:00 am, while we are sleeping. Most nights @ 1:15 am I get a notification on my phone "Roller Brush Stuck". Well obviously I don't see the notification until morning. I go "find" Eufy (which is easy with the app - shows you right where she is - another nice thing compared to the Shark) Pull the Roller Brush, which mind you, nothing is wrong with, it is not stuck, there is nothing wrapped around it etc. Put it back in & restart. This has done this since day one, brand new out of the box. After doing some scouring of the Internet, I see that this is a big complaint with many other owners of this model. From what I gather, Eufy is aware of this and it is a known problem. I called Eufy Customer Care after about using this for one week. The phone call lasted over 45 minutes (part of my life that I will never get back!) Now mind you, the representative was very helpful & thorough. We walked through a couple procedures, (The Rep had me pull the roller brush etc & report to her what I was "seeing") Which again, I had performed everything the Rep had me do religiously for the past week, but No, she like wouldn't take my word for it, I had to do all this again while on the phone with her - - almost like she didn't believe me at first. Next, the Rep offered to send me a "newer design roller brush" Ok, cool, if it solves the problem let's do it - I just wanted to get off the phone & on with my day. Oh wait, there are no new design roller brushes in stock. Now I'm told to send just the robot back (not the dock) I told the Rep to send me a call tag, as I wasn't going to pay shipping on a "brand new used one week" item. Oh wait, now she has located the replacement roller brushes. So here we are back to the original solution, she will send me a replacement roller brush. Took my name, my address, phone number, name of first & last born children etc. Just a long drawn out process to send me the "fix" for their known manufacture problem. So to make my long story even longer, here I sit a month and a 1/2 later without ever receiving the replacement brush and an unreliable product who doesn't complete her "mission" most nights. I wish this vacuum was still within the 30 day Amazon return period, because I would have sent it back after experiencing this defect. However my husband had bought this for me for Christmas (before the date that Amazon offers their extended holiday returns window) I didn't even unbox this vacuum before the 30 days were up.
The App with the mapping & the expansive scheduling options is great compared to the Shark, but I bought the Eufy to vacuum, which she fails @ nightly.
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Reviewed in the United States on February 5, 2026
★★★★★ 5
Great upgrade from my old robot
Size: Freo S, Size: Freo S
I had a different robot vacuum for a little over four years, and it finally started giving out, so I decided to try this one. It’s definitely an upgrade. The first thing I noticed is that it runs much quieter than my old one, which I really appreciate.
The self-emptying feature is super convenient as I don’t have to dump the container every single day like I used to. It mapped my home surprisingly fast. It moved through each room for a minute or two and somehow had the whole layout figured out almost immediately. I did adjust the room boundaries a bit, but nothing complicated.
Cleaning performance has been solid. I usually run it on the highest suction because I have a cat and a couple of carpeted rooms, and it handles the hair pretty well. For hardwood, there are lower modes too. It also has a mopping feature, but I don’t use it—I prefer my regular steam mop for that.
Setup was easy, and connecting it to the app took just a moment. And it charges quickly too.
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Reviewed in the United States on December 7, 2025
★★★★★ 5
Great value, Easy Set-up
Size: Freo S, Size: Freo S
I just got this vacuum and honestly, I’m pretty happy with it for the price. Here’s how it’s been going so far.
Price: This thing is a steal. Works just as well as some of the more expensive ones I’ve had.
Set up: It took maybe 5 minutes to get it going, and I didn’t even need to look at the manual. Super easy to set up and start mapping.
Carpet: Does a great job picking up dog hair and dust on my low-pile carpet. Doesn’t miss a spot.
Thresholds: It goes over low doorways and small lips—no getting stuck.
Navigation: It uses structured light for obstacle avoidance, which is typically found in much higher-end models, and it really exceeded my expectations. The cleaning path is smooth, and it avoids furniture without bumping into anything. It just works.
Base station: Very hands-off. It docks and charges itself. Plus, I love how sleek and simple it looks.
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Reviewed in the United States on September 11, 2025