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Description
Human IL-17A Kit (HICA)Product Specification Stability & Storage Store at 2~8C protected from light for 12 months; after reconstitution, the standard can be aliquoted and stored at 20C, avoiding repeated freeze thaw cycles. Background Test Principle: This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) based on the double antibody sandwich method for the detection of cytokine concentrations. The operation is simple and requires no washing steps. The
Product Specification
| Stability & Storage | Store at 2~8°C protected from light for 12 months; after reconstitution, the standard can be aliquoted and stored at -20°C, avoiding repeated freeze-thaw cycles. |
Background
Test Principle:
This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) based on the double-antibody sandwich method for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
The detection system includes two types of microspheres: acceptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two types of microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated by light excitation can transfer to the acceptor microspheres and trigger chemiluminescence. Conversely, if the target protein is absent, the distance between the microspheres is too large, and no signal is produced.
By measuring the intensity of the chemiluminescent signal, the target protein can be quantitatively analyzed. This method is characterized by its simplicity, rapid reaction, and high sensitivity.
Components
Here is the translated content in English, maintaining the original HTML table structure:```html
Name |
Specification |
Component Specification |
Detection Reagent R1 |
500T |
2mL/bottle×1 |
2000T |
8mL/bottle×1 |
|
10000T |
40mL/bottle×1 |
|
Detection Reagent R2 |
500T |
2mL/bottle×1 |
2000T |
8mL/bottle×1 |
|
10000T |
40mL/bottle×1 |
|
Detection Reagent R3 |
500T |
5mL/bottle×1 |
2000T极 |
20mL/bottle×1 |
|
10000T |
100mL/bottle×1 |
|
Standard |
500T |
0.15μg lyophilized×1 |
2000T |
0.15μg lyophilized×2 |
|
10000T |
0.15μg lyophilized×5 |
|
Standard Buffer |
500T |
6mL/bottle×1 |
2000T |
12mL/bottle×1 |
|
10000T |
30mL/bottle×1 |
Note: It is recommended to use microplates (384 or 96 well plates, white, shallow wells) for the assay.
```Guidelines
Reagent R3 must be protected from light. It is recommended to perform sample addition and incubation under green light (<100 LUX).
Recalibration is required for each test. At least two replicate wells should be set for each concentration point of the standard, and a four-parameter (weight 1/Y²) fitting calculation should be applied.
Temperature and time must be controlled during incubation. Microplates should be covered with a film, and a microplate reader with ALPHA function is recommended.
The dilution matrix of the calibrator should match the test samples. Reconstituted calibrators must be used within 2 hours.
Components from different reagent kit lots must not be mixed.
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