Pay in installments of $51.59 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 22 - Aug 27
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
EPI ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. 3. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and harvest by centrifugation at 1000 × g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Wash harvested cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1 × 10^6 cells (protease inhibitors are recommended; if the concentration is very low, the PBS volume can be reduced appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and harvest the supernatant for assay. 4. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, take the supernatant for detection, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and assay. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000 pg/mL). Then dilute to the following concentrations: 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 2000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day. 4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day. 5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C. 2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.) 3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes. 5. Wash: Discard the liquid and wash the plate five times as in step 3. 6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculation of experimental results: Result evaluation: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Sensitivity | 15.8 pg/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal species Epinephrine (EPI) antigen. After incubation and washing, the assay is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the universal species Epinephrine (EPI) present in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | All | |||||||||||||||||||||||||||||||||
| Synonym | General Epinephrine ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Epinephrine (L-3,4-dihydroxy-alpha-benzyl alcohol) is a hormone and neurotransmitter secreted by the adrenal medulla, as well as a medication. Epinephrine is used to treat a variety of conditions, including anaphylaxis, cardiac arrest, and superficial bleeding. Inhaled epinephrine is sometimes used to improve the symptoms of croup. Epinephrine may also be considered for asthma when first-line treatments are ineffective. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.2-2000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 21 reviews
Sort
Product Reviews
★★★★★ 4
Say goodbye to neck pain
Color: White, Size: Queen
This is a very good product but it takes some time to adjust removable stuffing to accommodate individual sleeping patterns.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 15, 2026
★★★★★ 5
Soft and comfortable cooling pillowcase that gets the job done!
Color: White, Size: Queen, Color: White, Size: Queen
I got this quilted bamboo pillow protector (queen size) because I wanted a cleaner, cooler layer between my pillow and pillowcase since I’m always hot. This has been a great solution for that.
The fabric feels soft and smooth, and it doesn’t make that crinkly noise some cooling pillowcases do. I’m a warm sleeper and the “cooling” aspect feels more like breathable than icy-cold, but it definitely sleeps more comfortably than the thick polyester protectors I’ve used in the past. The light quilting adds a little cushion without making my pillow feel bulky or changing the loft much.
I like the fact that if you use a memory foam fill pillow you can add or remove some of the filling to make the pillow as firm or mushy as you want. This is especially helpful for people that are side sleepers vs people that need more neck support.
The zipper is a big plus. Just be careful when zipping it open and closed as these are usually the first point of failure for pillowcases in my experience. It closes fully and keeps the protector snug so it doesn’t shift around inside the pillowcase, and it gives me peace of mind for sweat / oils and everyday wear. I’ve washed it and it came out fine with no weird shrinking, and the stitching still looks clean.
For the price, it feels like good value if you’re trying to extend the life of a nicer pillow while keeping the sleep surface soft and breathable. It’s one of those small upgrades you don’t think about much once it’s on, which is kind of the point. It just works and helps me sleep more comfortably.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 1, 2026
★★★★★ 5
Soft and cooling
Color: White, Size: King, Color: White, Size: King
This pillow cover is great! Well made. Not too thin. Fits my queen pillow like a glove. Came out of the washing machine still looking brand new, with no zipper issues, which can happen with lower quality covers. Zero complaints.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 21, 2026
★★★★★ 5
Soft, Cooling Pillowcase That Smooths Out Chunky Filling
Color: White, Size: King, Color: White, Size: King
I accidentally ordered the king size instead of queen, but it still fits my shredded latex foam pillow fairly well and feels comfortable. The pillow has small but chunky latex shreds, and the added foam layer in this pillowcase not only smooths out those chunks but also adds a bit of extra thick comfort, making the pillow feel more uniform.
It seems to help with temperature regulation—if anything, it’s subtly cooler or at least not any warmer than my previous pillowcase. I’ve washed it twice so far, and it shows no wear and seems well built, so it should hold up over time and seems totally washable. Overall, it’s a very functional item, and compared with similar products, the value seems reasonable; I’m happy with the purchase and just wish I had ordered the right size.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 23, 2025
★★★★★ 4
Good quality cover
Color: White, Size: Queen, Color: White, Size: Queen
First, this pillow cover arrived in a plastic bag (see 4th photo), not a box as the photos online show. Although that does not matter to me, it may matter to other people such as those who intend to give this as a gift.
One of the photos I included shows the fabric makeup which is mostly polyester and memory foam with the outer cover having “40% Bamboo Rayon.” It seems like an overstatement to me to market this product as a “quilted bamboo pillow cover” where there’s very little, if any, actual bamboo in it. If you have read up on what “bamboo rayon” actually is, you’ll understand.
The material make up aside, the cover is soft and puffier than a cotton pillowcase. Bamboo rayon does have qualities that allow airflow for a cooling type of effect. It washes very nicely.
As for the size of the pillar cover, I have tried several pillows inside this case, and the case is at least 2 inches longer than every standard pillow I own (See second photo). Pillowcases with a zipper generally are more fitted in every dimension. This pillow cover fits in all dimensions, except for it is longer than necessary. Not a horrible thing, but worth mentioning.
The sewing of this product is good. It has a standard plastic zipper. There’s piping around the entire seam area for touch of class. The gray piping on mine matches the quilting. This is not quilting of the standard sense where threads go through to the other side. It is more of a faux quilting, which you can that on the inside in the photo with the tag.
Overall, it seems to be a good product especially for people who sweat when they sleep.
Quilted Bamboo Pillow Cover Collection – Cooling Pillow Protector with Zipper – Soft, Breathable & Washable Fabric (White, Queen)
$19.99 at the time of this review
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 7, 2025