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Description
Rat NDRG2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with protein NDRG2 (NDRG2) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of protein NDRG2 (NDRG2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Protein NDRG2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Nmyc downstream regulated gene 2, also known as NDRG2, is the protein encoded by the NDRG2 gene. This gene is a member of the N-myc downregulated gene family, belonging to the α/β hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in the development of glioblastoma and aggressive meningioma carcinomas (through cell proliferation). Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.1 ★★★★★
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★★★★★ 5
Part of my daily routine
Style: Original, Size: 25 Count (Pack of 2)
These wipes are the BEST for removing makeup. I have tried others, but these are the perfect product for 1) effective cleansing; 2) convenience; and 3) value.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Neutrogena Micellar Makeup Remover Wipes—Effective and Gentle
Style: Original, Size: 25 Count (Pack of 2)
These makeup remover wipes are a high-quality choice for daily skincare. The micellar, alcohol-free formula is specifically designed to be gentle on the skin while still being powerful enough to remove stubborn, waterproof mascara without aggressive scrubbing. It leaves the face feeling clean and refreshed without any oily residue or irritation.
The convenience of these towelettes makes them a practical staple for a nightly routine or for travel. They are consistently effective and reliable, providing a soft touch that works well even for those with more sensitive skin. It is clearly a well-loved product that delivers on its promises of efficiency and comfort.
Bottom Line: A dependable and gentle skincare essential. These wipes offer a great balance of strength and softness, making them a favorite for anyone looking for an easy and effective way to clear their skin at the end of the day.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
I'm been using this make up remover for years!
Style: Original, Size: 25 Count (Pack of 2)
I’ve been using Neutrogena Makeup Remover for a while now, and it does an amazing job at removing makeup quickly and easily. It takes off everything—even waterproof mascara—without needing to rub too hard.
What I really like is how gentle it feels on my skin. It doesn’t leave any irritation or greasy residue, and my face feels clean and refreshed after using it.
It’s also super convenient, especially the wipes they’re perfect for travel or those nights when you’re too tired for a full routine.
The only downside is that the wipes can dry out if the package isn’t sealed properly, so make sure to close it tightly.
Overall, a reliable and effective makeup remover that I always keep on hand!
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Reviewed in the United States on March 17, 2026
★★★★★ 4
Not good for sensitive skin
Style: Original, Size: 25 Count (Pack of 2)
Not great for sensitive skin. But great for cleaning brushes or removing makeup if you have nothing else. Left my sensitive skin burning.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Gentle on my skin, tough at removing makeup.
Style: Original, Size: 25 Count (Pack of 2)
I've tried many different kinds of makeup remover wipes in my day, but I've never used these Neutrogena ones. As I was looking for some wipes to try out (as I'd recently used up all the ones I had), I decided to ordered the Neutrogena ones.
These packs came very well presented, and were in excellent condition. I appreciate that the wipes were wrapped in a plastic-type wrap, which protected them from the elements.
The wipes feel so soft and gentle on my skin; each cloth wipe feels durable, and is great at removing stuck-on makeup. These not only remove makeup very well, but they leave my skin feeling soft and hydrated.
I've not had any issues with irritations while using on my skin, and there's also no fragrance to the wipes (which may be of some importance to certain people).
I love that these wipes are made from plant-based materials, and they are compostable. The cloths feel durable yet lightweight, and have plenty of essence on each wipe. Additionally, these packs of wipes are convenient got on-the-go purposes.
I'm really happy with these Neutrogena makeup remover wipes, and feel they're of excellent quality and value. I feel these are a good value because this came with 2 packs (each with 25 wipes), and one wipe can do a lot.
At the time of my purchase (and review), these wipes were listed at around $7.98; I personally think this is a very nice deal, and would definitely consider ordering them again. I also would highly recommend these to others.
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Reviewed in the United States on March 31, 2026