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Description
Mouse XBP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an X-Box Binding Protein 1 (XBP1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of X-Box Binding Protein 1 (XBP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse X-Box Binding Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | X-box binding protein 1, also known as XBP1 or TREB5, is a protein encoded by the XBP1 gene. The XBP1 gene is located on chromosome 22, while a closely related pseudogene has been identified and mapped to chromosome 5. This protein is a transcription factor that regulates the expression of genes important for the normal functioning of the immune system and cellular stress responses. It is a bZIP domain-containing transcription factor. It was first identified by its ability to bind to Xbox, a conserved transcriptional element in the human leukocyte antigen (HLA) DRα promoter. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.5 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 4
Good quality. Works fine.
Color: Yellow, Color: Yellow
I've never had matcha or made it before, so I looked up how to care for this set and how to use it right. There's a website called "tea is the cup of life" that has a very informative video and article about how everything related to matcha is hand made, etc. And shows what you need to do. The instructions are also on box, but seemed simplified. They're accurate enough to get you started.
Based on the time consuming nature of hand making the bamboo stuff, I'd be really surprised if this is actually hand made with them selling the volume they're selling. It's still good quality, just I doubt it's hand made at this price point.
The one star off is for two reasons: the whisk is supposed to make the matcha "frothy". It got a little bubbly, but not more than just stirring it with a spoon or a regular little metal whisk does. The second reason is the bamboo has supposedly less static cling for the matcha powder compared to metal spoons, so you don't waste the labor intensive and expensive powder.
The matcha powder sticks to both bamboo spoons just as much as it sticks to a metal spoon, but the metal spoon rinsed off into the tea cup 100% and the bamboo still had a little powder in the grain so the spoon needed washed and a little powder was wasted. Is it enough to worry about really? I dunno. But it seems like the purists are really interested in details like that, so I'm mentioning it.
Oh yeah, the description says this one is yellow and their images show that yellow green crayon color, which is what I expected to get. It's that more green color. I don't think anyone would call it yellow when they see it in person.
The kit works as intended and it is good quality. Since I'm just getting into it for the health benefits of matcha tea and not for the ceremony, I didn't find the processes "meditative" at all.
I'll probably just stick to using a regular spoon and whisk in the cup, but if you want to do the whole ceremonial shebang at an affordable price, this kit will do the trick.
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Reviewed in the United States on September 27, 2025
★★★★★ 5
Great gift set to for matcha lovers
Color: Yellow
Got as a birthday gift. It's the recipient favorite color. Its not part of their daily routine to have a barista like matcha. And appreciate everything about making the quality of the matcha taste that much better. Good value got it on sale. I probably purchase another color for myself.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Matcha Set Highly recommended
Color: Pink
This is perfect for mixing your matcha and cold cream! My granddaughter is 11 and she is obsessed with matcha. Its perfect for her. Very cute set up!
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Reviewed in the United States on January 20, 2026
★★★★★ 5
Tea
Color: Yellow
A matcha set like the 8-piece ceramic speckled design (with teapot + whisk) is all about completeness, ceramic quality, included tools, and gift presentation. The best sets give you everything needed for a traditional matcha routine while also looking beautiful enough to display.
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Reviewed in the United States on April 18, 2026
★★★★★ 5
Cute
Color: Pink
This matcha set feels like good value for the price and works well for making matcha at home. The quality is solid and everything feels durable enough for regular use. The color is really pretty and the design is simple but cute. All the pieces do what they’re supposed to and fit together nicely. It’s a nice set if you want something functional that also looks good.
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Reviewed in the United States on December 21, 2025