SKU: 47002108700

UniOne® TR-FRET Human CDK2/CRBN PROTAC Binding Kit

Sale price$328.50 Regular price$365.00
Save 10%

Pay in installments of $91.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Sep 5 - Sep 10

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

UniOne® TR-FRET Human CDK2/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit employs homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between the molecular glue CDK2 Degrader7 (or test compounds) and the DDB1 CRBN complex with CDK2. This method enables simple and rapid high throughput screening of small molecules capable of mediating the interaction between the DDB1 CRBN complex and CDK2. As shown in the

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the molecular glue CDK2 Degrader7 (or test compounds) and the DDB1/CRBN complex with CDK2. This method enables simple and rapid high-throughput screening of small molecules capable of mediating the interaction between the DDB1/CRBN complex and CDK2.

As shown in the figure below, the interaction between DDB1/CRBN and CDK2 is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The molecular glue CDK2 Degrader7 mediates the interaction between DDB1/CRBN and CDK2, bringing the donor and acceptor antibodies into proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. This signal is proportional to the extent of interaction mediated by CDK2 Degrader7 between DDB1/CRBN and CDK2. The homogeneous assay is straightforward and requires no washing steps.

Components

Product Components and Storage Conditions】

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Tag1-CRBN protein

10

5μL

20μL

100μL

400μL

-80℃

Tag2-CDK2 protein

100×

5μL

20μL

100μL

400μL

-80℃

CDK2 Degrader 7

1mM

5μL

5μL

25μL

100μL

-80℃

Anti-Tag1 Eu antibody

5

5μL

50μL

250μL

1mL

-80℃

Anti-Tag2 Ac antibody

100×

5μL

25μL

125μL

500μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: All components should be aliquoted immediately after the first thaw and stored at the recommended temperature to avoid dilution storage and repeated freeze-thaw cycles.

Protocol

Here is the translated content in English with all original formatting preserved:```html

1. Reagent Preparation

1.1 Thaw all reagents to room temperature before use (equilibrate for at least 30 min). The reaction system for 384-well shallow plates is 20 μL (reagent volumes are shown in the table below). Calculate the required volume before preparation. The following preparation is for reference only, using 500 reactions as an example.

Table 1. Reagent Preparation

Reagent Name

Preparation

Volume per Well (μL)

Detection buffer

Add 2 mL of 10× Detection buffer to 18 mL of deionized water, dilute to 1×, and mix well.

-

CDK2 Degrader7

Dilute the compound to the target concentration with 1× Detection buffer according to the reaction system.

2

Tag1-CRBN protein

Take 20 μL of Tag1-CRBN protein stock solution, dilute to 2 mL with 1× Detection buffer, and mix well.

4

Tag2-CDK2 protein

Take 20 μL of Tag2-CDK2 protein stock solution, dilute to 2 mL with 1× Detection buffer, and mix well.

4

Antibody Mix

Take 50 μL of Anti-Tag1 Eu antibody stock solution, add 2.450 mL of 1× Detection buffer, mix well; take 25 μL of Anti-Tag2 Ac antibody stock solution, add 2.475 mL of 1× Detection buffer, mix well; mix the two 1:1 to prepare the Antibody Mix.

10


1.2 Gradient Dilution of Test Samples

Using CDK2 Degrader7 as an example, the diluent is 1× Detection buffer. To minimize matrix interference, it is recommended to use a solution with the same matrix as the test sample for dilution. Adjust the dilution based on the actual concentration of the sample.

Table 2. Gradient Dilution of CDK2 Degrader7 (adjust as needed)

CDK2 Degrader7

Final Concentration (nM)

CDK2 Degrader7 Preparation Concentration (nM)

Preparation Method

1000

10000

1 μL of 1 mM CDK2 Degrader7 + 99 μL of 1× Detection buffer

333.3333

3333.333

20 μL of ① + 40 μL of 1× Detection buffer

111.1111

1111.111

20 μL of ② + 40 μL of 1× Detection buffer

37.0370

370.370

20 μL of ③ + 40 μL of 1× Detection buffer

12.3457

123.457

20 μL of ④ + 40 μL of 1× Detection buffer

4.1152

41.152极u>

20 μL of ⑤ + 40 μL of 1× Detection buffer

1.3717

13.717

20 μL of ⑥ + 40 μL of 1× Detection buffer

0.4572

4.572

20 μL of ⑦ + 40 μL of 1× Detection buffer

0.1524

1.524

20 μL of ⑧ + 40 μL of 1× Detection buffer

Blank

0

0

40 μL of 1× Detection buffer


2. Sample Loading and Controls

2.1 Test samples: Add 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-CDK2 protein working solution, 2 μL of gradient-diluted test sample, and 10 μL of mixed Antibody Mix sequentially into the 384-well shallow plate.

2.2 Positive control standard curve: Add 4 μL of Tag1-CRBN protein working solution, 4 μL of Tag2-CDK2 protein working solution, 2 μL of gradient-diluted CDK2 Degrader7, and 10 μL of Antibody Mix.

2.3 Blank control wells: Replace the test sample with 2 μL of 1× Detection buffer.

2.4 NC: Add 10 μL of 1× Detection buffer and 10 μL of Antibody Mix.

After all samples are loaded, centrifuge, seal with plate-sealing film, and incubate at room temperature for 2 hours.

Positive Control Standard Curve

Test Samples

Blank Control Wells

NC

2 μL

Gradient-diluted CDK2 Degrader7

2 μL

Gradient-diluted test sample

2 μL

1× Detection buffer

10 μL of 1× Detection buffer

plus 10 μL of Antibody Mix

4 μL of Tag1-CRBN protein

4 μL of Tag2-CDK2 protein

10 μL of Antibody Mix

Seal the plate with sealing film, and incubate at room temperature for 2 hours.


3. Detection

Measure using a TR-FRET-compatible microplate reader. Excitation wavelength is 320/340 nm, and emission wavelengths are 620 nm and 665 nm.

[Result Calculation]

1) Calculate the signal value (Ratio): Divide the 665 nm fluorescence signal by the 620 nm fluorescence signal and multiply by 10,000.

Ratio = (665/620) × 10,000

2) Calculate the Net signal based on the signal value:

Net signal = (Std - NC)/NC × 100

3) Calculate CV (%):

CV (%) = Standard Deviation/Mean Ratio × 100%

[Data Example]

The following data cannot replace experimental results and is provided as an example only. Results may vary depending on the plate reader used.  

Note: Recommended microplate (384-well plate, white, shallow wells).

```
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 47002108700

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.4 ★★★★★
Based on 15 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
A
Verified Purchase
Amanda Gilmore
Houston, US
★★★★★ 5
Best frother ever
Color: White
This brother is wickedly powerful you don't need to use it for very long to get phenomenal frothing results Incorporates ingredients quickly
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
G
Verified Purchase
GoGreen
Fort Morgan, US
★★★★★ 5
Works great and so much fun!
Color: Black
How did I live without this for so long? I love this little gadget! It gets used multiple times a day. It's not only great for frothing milk but it will whip eggs for a recipe and stir other ingredients as well. People who have complained that the stirring attachment was missing a piece did not read the description. The picture clearly shows there is no "boot" on that attachment, you don't want that one to spin like the frother. The Frother holds a charge for quite a while as well. I use mine multiple times a day and it is still holding a charge after weeks of daily use. Great item!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 15, 2026
N
Verified Purchase
Nick
Phoenix, US
★★★★★ 3
Disappointed
Color: White, Color: White
Powerful, but the description differs from the actual specifications, and the speed cannot be adjusted.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 15, 2026
B
Verified Purchase
Beach Dreamer
Boise, US
★★★★★ 5
The perfect whipper for protein powders
Color: Blue
Love, love this whipper. Had to finally replace my old one that I got several years ago that was battery operated. This rechargeable replacement is incredible. Thought I would miss my old one, but no. The 3 interchangeable attachments make it my go to for a ton of things. Perfect for mixing protein powders!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 23, 2026
H
Verified Purchase
Hanna
Belleville, US
★★★★★ 5
Still The Best Frother Ever!
Color: Black
I love how it is easy to use, how it holds a charge, and how much power it has. I've only charged it once since I got it & I've been using it on a daily basis!!! Though it isn't a long time, I'm happy with it so far! I definitely will recommend it to anyone looking for a great frother. If you are looking for one, look no further! You won't be disappointed! I've read some comments where they found it to be too strong that their drink spilled everywhere. But for me I have not had any incident of spilling over. I have used it both for my coffee and my matcha tea and it worked great without any spilling over. You just have to start with the lowest power and ensure that you have a large enough container to have a room for your frothing. My sister tried to use it recently during her visit to make us matcha tea. However, she experienced spillage all over the cup, because she had used a small cup. Otherwise, she liked it very much and said she will get one when her battery operated one stops working... Another thing I like about the product was the customer service that I had received. When I got my first order which came quickly, it wasn't working after having charged it over 24 hours. It was not turning on at all. I returned it and ordered a replacement which I received within a day or two. This time after charging it for the recommended amount of time, I was a bit skeptical when I turned it on. However, I was pleasantly surprised that it worked like it was supposed to and it has been working ever since having charged it only once so far. I may come back and give an update depending on how it does after 6 months of use, so keep in tune... I don't know if there is a way to write a new entry as a follow up to the old entry, so I'm going to add my follow up comment over here. May 23, 2025 - I love my frother more than I can say in words. It's functionality has not changed a bit from the time I have purchased it. It charge lasts several weeks to few months before I charge it again and the power is still the same. Recently I decided to buy another one for my office use. I like the white color and so tried it but that one wouldn't even turn on. And then I got a gray one to have a different color. That one was much weaker than the black one I own. Then I replaced it with another gray one within few days. I tried it and it was exactly the same as the one I already replaced. So then I decided to go with a black one again hoping that it would be the same as the one I have. The black one did much better than the gray ones in the strength or power of the frother. However, it is weaker than the one I already have been using since last August. While I am not very happy with the quality being inconsistent in all of the ones I have tried including the recent pictures of the black one, I have decided to keep the black one because it is relatively better than the gray ones I tried. What I don't understand is why there is inconsistency in the quality of the same product by the same maker. I am beginning to wonder if the black one I purchased in Augusta last year was anomaly of a quality and a good way. Maybe I lucked out on my older black frother, but I'm unsure if quality is going to get better if I were to order another one. I have a sister who is a pro in making coffee drinks and she was the reason I had ordered this frother to begin with. She uses a battery operated one at this time and I have been thinking about getting her one like mine. While I am still skeptical, I am going to give it a shot and order her one sometime soon. If it doesn't work, I guess we will return it back. But one thing that I am grateful is that I have been able to return every one of those that I have tried recently with no issues. I hope that I don't have to return my most recent purchase in black. I was not even able to review the new purchase because of the item being the same color which is crazy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 8, 2024

recommand products